Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,742,599 Δ1 bp intergenic (‑67/‑134) yiaJ ← / → yiaK transcriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon/2,3‑diketo‑L‑gulonate reductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,742,5980C.100.0% 41.1 / NA 11intergenic (‑66/‑135)yiaJ/yiaKtranscriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon/2,3‑diketo‑L‑gulonate reductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (5/6);  total (5/6)

GGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCAGATT  >  NC_000913/3742463‑3742638
                                                                                                                                       |                                        
ggCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                       <  1:325624/139‑4 (MQ=255)
         cTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTa                             >  1:1009883/1‑139 (MQ=255)
            gACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGt                          >  1:793005/1‑139 (MQ=255)
                     tttCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGaaa                 <  2:915695/139‑1 (MQ=255)
                       tCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAAtt               <  2:793005/139‑1 (MQ=255)
                             gcCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaa         >  1:392169/1‑139 (MQ=255)
                                    cGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt  <  1:1029495/139‑6 (MQ=255)
                                    cGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt  <  2:860824/139‑6 (MQ=255)
                                        ctctTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgt   <  1:741544/134‑5 (MQ=255)
                                        ctctTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACCATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgt   >  2:741544/1‑130 (MQ=255)
                                                                                          gcgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTg                    <  1:494954/67‑1 (MQ=255)
                                                                                          gcgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTg                    >  2:494954/1‑67 (MQ=255)
                                                                                                                                       |                                        
GGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCAGATT  >  NC_000913/3742463‑3742638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: