| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 723,528 | (T)8→9 | coding (887/2685 nt) | kdpD ← | fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 723,520 | 1 | . | T | 93.9% | 144.7 / 2.6 | 49 | coding (895/2685 nt) | kdpD | fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base T (19/27); total (21/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCG > NC_000913/723387‑723644 | ccGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑ttttt > 2:693351/1‑139 (MQ=255)ccGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑ttttt > 2:297299/1‑139 (MQ=255) gaATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑tttttt‑ttt < 1:693351/139‑2 (MQ=255) ttGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg < 2:413933/139‑1 (MQ=255) ttGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg < 1:969137/139‑1 (MQ=255) ttGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg < 1:794490/139‑1 (MQ=255) aTTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGt < 1:1078525/128‑1 (MQ=255) aTTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGt > 2:1078525/1‑128 (MQ=255) cACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAgggg < 2:485557/139‑1 (MQ=255) cGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGtt < 2:94695/139‑1 (MQ=255) cGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑TTTTTT‑TTCCGGTAAGCGGTg < 1:977476/125‑1 (MQ=255) cGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑TTTTTT‑TTCCGGTAAGCGGTg > 2:977476/1‑125 (MQ=255) cTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGTCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTT‑TTCCGGTAAGCGGTgc < 2:731620/115‑1 (MQ=255) cTACCGCTTTCTCTTCCGATGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTT‑TTCCGGTAAGCGGTgc > 1:731620/1‑115 (MQ=255) cGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTg < 1:297299/139‑1 (MQ=255) ttCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAg > 1:826801/1‑139 (MQ=255) tctcTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTgcagggcagg < 2:1057327/113‑1 (MQ=255) tctcTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTgcagggcagg > 1:1057327/1‑113 (MQ=255) tctcTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGtt > 1:567479/1‑117 (MQ=255) tctcTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGtt < 2:567479/117‑1 (MQ=255) ttCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg < 2:766229/95‑1 (MQ=255) ttCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg > 1:766229/1‑95 (MQ=255) gATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGcc > 2:504665/1‑139 (MQ=255) gAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGAc > 1:341315/1‑139 (MQ=255) aaGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTgcagggca < 1:1049751/92‑1 (MQ=255) aaGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTgcagggca > 2:1049751/1‑92 (MQ=255) aaGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGc > 1:523204/1‑139 (MQ=255) tgtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg > 1:135212/1‑77 (MQ=255) tgtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg < 2:135212/77‑1 (MQ=255) tgtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg > 2:293506/1‑77 (MQ=255) tgtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg < 1:293506/77‑1 (MQ=255) tGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTgcagggca > 2:442667/1‑86 (MQ=255) tGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTgcagggca < 1:442667/86‑1 (MQ=255) tcCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑TTTTTTGTTGCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGACAGCCGCGCCACt > 2:735264/1‑139 (MQ=255) aGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTgcagggca < 2:226107/70‑1 (MQ=255) aGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTgcagggca > 1:226107/1‑70 (MQ=255) aGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg > 1:844491/1‑46 (MQ=39) aGACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg < 2:844491/46‑1 (MQ=39) gACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg > 1:739901/1‑45 (MQ=38) gACGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAg < 2:739901/45‑1 (MQ=38) aCGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGcc < 1:125010/139‑1 (MQ=255) aCGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGcc < 2:826801/139‑1 (MQ=255) cGTAAGGCGCTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCg < 1:400579/139‑1 (MQ=255) cTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGtt > 2:321216/1‑52 (MQ=255) cTGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGtt < 1:321216/52‑1 (MQ=255) tGAGAATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGc < 1:694976/139‑1 (MQ=255) gagaATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgcc > 1:958680/1‑96 (MQ=255) gagaATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgcc < 2:958680/96‑1 (MQ=255) agaATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCg < 2:341315/139‑1 (MQ=255) agaATTGCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCg < 1:504665/139‑1 (MQ=255) gCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACa > 2:1024569/1‑49 (MQ=255) gCCCGACGT‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACa < 1:1024569/49‑1 (MQ=255) | CCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑TTTTTT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCG > NC_000913/723387‑723644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |