Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,080,915 (G)5→6 intergenic (+102/‑441) putP → / → efeU proline:sodium symporter/ferrous iron permease (pseudogene)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,080,9101.G100.0% 76.0 / NA 23intergenic (+97/‑446)putP/efeUproline:sodium symporter/ferrous iron permease (pseudogene)
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (15/8);  total (15/8)

CTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAGTTAGATTTGATAATCGCGGATGGACG  >  NC_000913/1080785‑1081043
                                                                                                                              |                                                                                                                                     
cTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGGCGGCCaa                                                                                                                           >  2:360239/1‑139 (MQ=255)
             gTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAg                                                                                                                                      >  2:767117/1‑113 (MQ=255)
             gTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAg                                                                                                                                      <  1:767117/113‑1 (MQ=255)
                        gCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCgccgccgcc                                                                                                                >  2:965135/1‑126 (MQ=255)
                        gCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCgccgccgcc                                                                                                                <  1:965135/126‑1 (MQ=255)
                              ggACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTc                                                                                             >  2:249050/1‑139 (MQ=255)
                                                                                         ccTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCctg                                                                                                           >  2:1021793/1‑66 (MQ=255)
                                                                                         ccTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCctg                                                                                                           <  1:1021793/66‑1 (MQ=255)
                                                                                           tCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCg                                                       <  2:932200/116‑1 (MQ=255)
                                                                                           tCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCg                                                       >  1:932200/1‑116 (MQ=255)
                                                                                             gCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGcccc                                                                                                              <  2:695802/59‑1 (MQ=25)
                                                                                             gCCATATAAATTGAATGATGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGcccc                                                                                                              >  1:695802/1‑59 (MQ=21)
                                                                                                           aTGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacc                                                               <  2:1072675/92‑1 (MQ=255)
                                                                                                           aTGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacc                                                               >  1:1072675/1‑92 (MQ=255)
                                                                                                           aTGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAg                            >  1:787701/1‑127 (MQ=255)
                                                                                                           aTGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAg                            <  2:787701/127‑1 (MQ=255)
                                                                                                            tGGTGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAGTTAGATTTGATaa               >  2:738638/1‑139 (MQ=255)
                                                                                                               tGCAGGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAGTTAGATTTGATAATcg            >  1:836211/1‑139 (MQ=255)
                                                                                                                        cgcgcAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTg                                                  <  2:277506/92‑1 (MQ=255)
                                                                                                                        cgcgcAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTg                                                  >  1:277506/1‑92 (MQ=255)
                                                                                                                        cgcgcAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAGTTAGATTTGATAATCGCGGATGGAc   >  2:536609/1‑139 (MQ=255)
                                                                                                                         gcgcAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGa                                                                      >  2:360578/1‑71 (MQ=255)
                                                                                                                         gcgcAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGa                                                                      <  1:360578/71‑1 (MQ=255)
                                                                                                                         gcgcAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAGTTAGATTTGATAATCGCGGATGGACg  >  2:299060/1‑139 (MQ=255)
                                                                                                                         gcgcAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAGTTAGATTTGATAATCGCGGATGGACg  >  1:472804/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                                     
CTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAGTTAGATTTGATAATCGCGGATGGACG  >  NC_000913/1080785‑1081043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: