Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,189,853 (T)7→8 coding (595/672 nt) phoP ← response regulator in two‑component regulatory system with PhoQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,189,8461.T100.0% 142.1 / NA 44I201N (ATT→AAT) phoPresponse regulator in two‑component regulatory system with PhoQ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (18/26);  total (18/26)

TGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAA  >  NC_000913/1189711‑1189978
                                                                                                                                        |                                                                                                                                    
tGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAttt                                                                                                                                   >  2:989973/1‑139 (MQ=255)
 gCCGTTGCCAACAGAAAACGTACCCGCAGAGAGAGCGGGAAAAAATGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAtttt                                                                                                                                  >  2:641674/1‑139 (MQ=255)
             aGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTTTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGAc                                                                                                                       <  1:641674/139‑1 (MQ=255)
                 aaCGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTcc                                                                                                                   <  2:708192/139‑1 (MQ=255)
                    gTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCAt                                                                                                                <  1:461114/139‑1 (MQ=255)
                    gTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCAt                                                                                                                <  1:618562/139‑1 (MQ=255)
                             gCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATc                                                                                                       <  1:650310/139‑1 (MQ=255)
                                     gggAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCTCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTAt                                                                                               <  1:625352/139‑1 (MQ=255)
                                                              tcatcaGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCgg                                                                      >  1:890450/1‑139 (MQ=255)
                                                                         aTTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGa                                                           <  1:288099/139‑1 (MQ=255)
                                                                                  gATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCAt                                                                                                                >  1:9817/1‑77 (MQ=255)
                                                                                  gATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCAt                                                                                                                <  2:9817/77‑1 (MQ=255)
                                                                                    tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGa                                                                                                                        <  1:361183/67‑1 (MQ=255)
                                                                                    tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGa                                                                                                                        >  2:361183/1‑67 (MQ=255)
                                                                                           ggCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGa                                                                     >  1:1019449/1‑111 (MQ=255)
                                                                                           ggCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGa                                                                     <  2:1019449/111‑1 (MQ=255)
                                                                                           ggCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGc                                         >  1:140064/1‑139 (MQ=255)
                                                                                             ccGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCg                                                                                     <  1:600555/93‑1 (MQ=255)
                                                                                             ccGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCg                                                                                     >  2:600555/1‑93 (MQ=255)
                                                                                                 gAACGGTGGTAATCACTTCTTGGGGATATTTTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCAtt                                                      <  2:820912/120‑1 (MQ=255)
                                                                                                 gAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCAtt                                                      >  1:820912/1‑120 (MQ=255)
                                                                                                    cGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACtt                                <  2:140064/139‑1 (MQ=255)
                                                                                                       tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGc                             >  2:1101034/1‑139 (MQ=255)
                                                                                                                 ttcttGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCg                                                                                     <  2:62087/73‑1 (MQ=255)
                                                                                                                 ttcttGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCg                                                                                     >  1:62087/1‑73 (MQ=255)
                                                                                                                 ttcttGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGa                                                                     >  2:531151/1‑89 (MQ=255)
                                                                                                                 ttcttGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGa                                                                     <  1:531151/89‑1 (MQ=255)
                                                                                                                 ttcttGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCg                   >  2:924666/1‑139 (MQ=255)
                                                                                                                 ttcttGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCg                   >  1:28546/1‑139 (MQ=255)
                                                                                                                   cttGGGGATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTa                 >  2:716407/1‑139 (MQ=255)
                                                                                                                        ggATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc            <  1:989973/139‑1 (MQ=255)
                                                                                                                        ggATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc            <  2:28546/139‑1 (MQ=255)
                                                                                                                        ggATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc            <  2:890450/139‑1 (MQ=255)
                                                                                                                        ggATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc            <  1:470071/139‑1 (MQ=255)
                                                                                                                        ggATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGGTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc            <  2:685744/139‑1 (MQ=255)
                                                                                                                         gATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATc                                                                                                               <  2:323964/39‑1 (MQ=37)
                                                                                                                         gATATTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATc                                                                                                               >  1:323964/1‑39 (MQ=37)
                                                                                                                          atatTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGt          <  1:564371/139‑1 (MQ=255)
                                                                                                                           tatTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTg                                       <  1:953746/109‑1 (MQ=255)
                                                                                                                           tatTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTg                                       >  2:953746/1‑109 (MQ=255)
                                                                                                                           tatTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc            <  1:125200/136‑1 (MQ=255)
                                                                                                                           tatTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc            >  2:125200/1‑136 (MQ=255)
                                                                                                                           tatTGTGCCTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGtt         >  1:340698/1‑139 (MQ=255)
                                                                                                                                  ccTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCg                                                                             <  2:170839/64‑1 (MQ=255)
                                                                                                                                  ccTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCg                                                                             >  1:170839/1‑64 (MQ=255)
                                                                                                                                  ccTGAATTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCataa  <  1:1101034/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAA  >  NC_000913/1189711‑1189978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: