Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,690,107	+A	coding (256/1509 nt)	ydgA →	DUF945 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,690,103	1	.	A	100.0%	115.4 / NA	37	I84I (ATT→ATA)	ydgA	DUF945 family protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (17/20);  total (17/20)

GCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGAATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATT‑AAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCA  >  NC_000913/1689965‑1690236                                                                                                                                            |                                                                                                                                       gcAGGCGAACGCGCAACTCAAACTGACAGCTCCTGAATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGAtt                                                                                                                                         >  2:890292/1‑139 (MQ=255)               aaCTCAAACTGACAGCTCCTGAATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCaga                                                                                                                          >  1:1015574/1‑139 (MQ=255)                          aCAGCTCCTGAATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTc                                                                                                               <  1:753186/139‑1 (MQ=255)                               tCCTGAATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGa                                                                                                          <  1:497886/139‑1 (MQ=255)                                      tCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGtt                                                                                                   <  1:890292/139‑1 (MQ=255)                                          aCCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATc                                                                                               >  1:922008/1‑139 (MQ=255)                                           ccTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGAGAACGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCa                                                                                              <  2:560999/139‑1 (MQ=255)                                                aaGCGAGTTATCAAAACTAGCATCGCGGCGTATTTAGCAGCCAGTTGCAACTGTTGGTGAAACCCATCGCCGGGAAAGAAGATCCGGGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATTGGTTGATCATGGTc                                                                                         >  2:678552/1‑139 (MQ=255)                                                     aGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTc                                                                                    >  1:221060/1‑139 (MQ=255)                                                              aaCTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGcc                                                                           <  1:581640/139‑1 (MQ=255)                                                                   tcatcGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGcc                                                                           >  1:233180/1‑134 (MQ=255)                                                                   tcatcGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGcc                                                                           <  2:233180/134‑1 (MQ=255)                                                                         cggcgTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTaaaaa                                                                >  2:998073/1‑139 (MQ=255)                                                                          ggcgTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTaaaaaa                                                               <  1:998073/139‑1 (MQ=255)                                                                           gcgTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAAc                                                              <  2:922008/139‑1 (MQ=255)                                                                                     cagcCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCg                                                                                                                       <  1:419439/72‑1 (MQ=255)                                                                                     cagcCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCg                                                                                                                       >  2:419439/1‑72 (MQ=255)                                                                                      agcCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGCAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATc                                                   <  2:700108/139‑1 (MQ=255)                                                                                      agcCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATc                                                   <  2:34522/139‑1 (MQ=255)                                                                                             tGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCa                                                                                                                            <  1:438829/59‑1 (MQ=255)                                                                                             tGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCa                                                                                                                            >  2:438829/1‑59 (MQ=255)                                                                                                  cTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCa                                       >  2:139917/1‑139 (MQ=255)                                                                                                   tGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCAt                                      <  1:139917/139‑1 (MQ=255)                                                                                                      tGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGt                                                                                                                              >  1:804926/1‑48 (MQ=39)                                                                                                      tGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGt                                                                                                                              <  2:804926/48‑1 (MQ=255)                                                                                                       ggTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAAt                                                                                                        <  2:157546/69‑1 (MQ=255)                                                                                                       ggTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAAt                                                                                                        >  1:157546/1‑69 (MQ=255)                                                                                                             aCCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAAc                            >  2:447180/1‑139 (MQ=255)                                                                                                             aCCCATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAAc                            >  1:201754/1‑139 (MQ=255)                                                                                                              cccATTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAAca                           >  2:106361/1‑138 (MQ=255)                                                                                                                 aTTGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACg                        <  2:306514/139‑1 (MQ=255)                                                                                                                   tGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGaa                                                                                                         <  2:459742/56‑1 (MQ=255)                                                                                                                   tGCCGGGAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGaa                                                                                                         >  1:459742/1‑56 (MQ=255)                                                                                                                        ggAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCAt                                                                                                                   <  2:484419/41‑1 (MQ=255)                                                                                                                        ggAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCAt                                                                                                                   >  1:484419/1‑41 (MQ=255)                                                                                                                        ggAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGt                                                                                                    >  1:746218/1‑56 (MQ=255)                                                                                                                        ggAAAGAAAATCCGTGGATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGt                                                                                                    <  2:746218/56‑1 (MQ=255)                                                                                                                                aaTCCGTGGATT‑AAAGAGCGGGCAGAGCGTCATCTTCAACGAATCGGTTGAGCATGGTGCCTCCGCGCTTGCCCAGCTTAAAAAACTGAACCTGAGCCCGTCGATGGCATCAATTCAAACCGCACTGGTTAATAACGaa         <  1:678552/139‑1 (MQ=255)                                                                                                                                       ggATTAAAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCa  <  2:201754/139‑1 (MQ=255)                                                                                                                                            |                                                                                                                                       GCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGAATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATT‑AAA‑AGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCA  >  NC_000913/1689965‑1690236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑