Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,700,201 (T)5→6 coding (847/1593 nt) malX → maltose and glucose‑specific PTS enzyme IIB component and IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,700,1961.T100.0% 62.2 / NA 21G281V (GGT→GTT) malXmaltose and glucose‑specific PTS enzyme IIB component and IIC component
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (8/13);  total (8/13)

CCGTTTGGTCTGCATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCT  >  NC_000913/1700060‑1700328
                                                                                                                                         |                                                                                                                                    
ccGTTTGGTCTGCATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGtt                                                                                                                                    <  2:1062961/139‑1 (MQ=255)
                   ttCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCAcgc                                                                                                                  <  2:156864/139‑1 (MQ=255)
                            cATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCttt                                                                                                         >  1:48901/1‑139 (MQ=255)
                               tAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGc                                                                                                      <  1:365849/139‑1 (MQ=255)
                                                                 aGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGcc                                                                    <  2:887551/139‑1 (MQ=255)
                                                                 aGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCCGCGTTTCTCGGCGGTCTGcc                                                                    <  1:640085/139‑1 (MQ=255)
                                                                     tGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGt                                                                >  1:58924/1‑139 (MQ=255)
                                                                      gCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTg                                                               <  1:828391/139‑1 (MQ=255)
                                                                       cGGTCAAACCGTCAGCGGCGCACTGACGATCTCCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGTTCTGCCAGGTGc                                                              >  2:661960/1‑139 (MQ=255)
                                                                          tCAAACCGTCAGCGGCGCACTTACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTGTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTCCAGc                                                           <  2:646803/139‑1 (MQ=255)
                                                                             aaCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGtt                                                        >  1:307707/1‑139 (MQ=255)
                                                                              aCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTa                                                       >  2:923453/1‑139 (MQ=255)
                                                                                 gTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCt                                                    <  2:188053/139‑1 (MQ=255)
                                                                                 gTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCt                                                    <  2:48901/139‑1 (MQ=255)
                                                                                                        ccAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTg                                                                                       >  2:500037/1‑81 (MQ=255)
                                                                                                        ccAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTg                                                                                       <  1:500037/81‑1 (MQ=255)
                                                                                                                         gCCCGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTa            <  2:595649/139‑1 (MQ=255)
                                                                                                                          cccGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg                                                      <  2:822838/96‑1 (MQ=255)
                                                                                                                          cccGACCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg                                                      >  1:822838/1‑96 (MQ=255)
                                                                                                                              aCCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg                                                      <  2:514842/92‑1 (MQ=255)
                                                                                                                              aCCACTCACGGTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg                                                      >  1:514842/1‑92 (MQ=255)
                                                                                                                                   tCACGGTTTTTTCTGAAAGCGCCACTCGTTACCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTctgct  <  2:1017889/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                    
CCGTTTGGTCTGCATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCT  >  NC_000913/1700060‑1700328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: