Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,972,647	(C)5→6	coding (45/1662 nt)	tar ←	methyl‑accepting chemotaxis protein II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,972,642	1	.	C	100.0%	86.7 / NA	25	V17G (GTA→GGA)	tar	methyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (11/14);  total (11/14)

TTAAATCCCAGGTTGACGTCAGCTCGCCCTGCTGTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑CCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCG  >  NC_000913/1972514‑1972768                                                                                                                                  |                                                                                                                               ttAAATCCCAGGTTGACGTCAGCTCGCCCTGCTGTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑ccccccagca                                                                                                                      <  2:599703/139‑6 (MQ=255) ttAAATCCCAGGTTGACGTCAGCTCGCCCTGCTGTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑ccccccagca                                                                                                                      <  1:572499/139‑6 (MQ=255)           ggTTGACGTCAGCTCGCCCTGCTGTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCaa                                                                                                             <  1:1017877/139‑1 (MQ=255)           ggTTGACGTCAGCTCGCCCTGCTGTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCaa                                                                                                             <  2:542426/139‑1 (MQ=255)                 cGTCAGCTCGCCCTGCTGTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑c                                                                                                                               >  1:319235/1‑114 (MQ=255)                 cGTCAGCTCGCCCTGCTGTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑c                                                                                                                               <  2:319235/114‑1 (MQ=255)                              tgctgTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATa                                                                                          <  2:421217/139‑1 (MQ=255)                                ctgTTCCCGTAATTGATTGGAATCCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑cc                                                                                                                              <  2:702929/100‑1 (MQ=255)                                ctgTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACg                                                                                        <  1:819437/139‑1 (MQ=255)                                ctgTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑cc                                                                                                                              >  1:702929/1‑100 (MQ=255)                                 tgTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACgg                                                                                       >  1:65771/1‑139 (MQ=255)                                   ttCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGtt                                                                                     >  2:626278/1‑139 (MQ=255)                                               attgGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATa                                                                                          <  1:598300/122‑1 (MQ=255)                                               attgGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATa                                                                                          >  2:598300/1‑122 (MQ=255)                                               attgGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑ccccccagca                                                                                                                      <  1:779010/93‑6 (MQ=255)                                               attgGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑ccccccagca                                                                                                                      >  2:779010/1‑88 (MQ=255)                                                      aCCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCt                                                                  >  1:532267/1‑139 (MQ=255)                                                                  ttctGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑ccccccag                                                                                                                        >  1:673372/1‑69 (MQ=255)                                                                  ttctGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑ccccccag                                                                                                                        <  2:673372/72‑4 (MQ=255)                                                                         tATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATa                                                                                          <  2:365337/96‑1 (MQ=255)                                                                         tATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATa                                                                                          >  1:365337/1‑96 (MQ=255)                                                                            ggggAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTAt                                            >  1:837376/4‑139 (MQ=255)                                                                            ggTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATa                                                                                          <  1:429793/93‑1 (MQ=255)                                                                            ggTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATa                                                                                          >  2:429793/1‑93 (MQ=255)                                                                                 aGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCa                                       <  2:68745/139‑1 (MQ=255)                                                                                      agaaAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATa                                                                                          >  2:109453/1‑83 (MQ=255)                                                                                      agaaAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATa                                                                                          <  1:109453/83‑1 (MQ=255)                                                                                       gaaAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGt                                                                                      >  1:1054981/1‑86 (MQ=255)                                                                                       gaaAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGt                                                                                      <  2:1054981/86‑1 (MQ=255)                                                                                            aaaCAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGa                            <  2:837376/139‑1 (MQ=255)                                                                                                  aCTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAg                                                         >  1:857695/1‑104 (MQ=255)                                                                                                  aCTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAg                                                         <  2:857695/104‑1 (MQ=255)                                                                                                                    tAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGaaa                                                              >  1:95592/1‑81 (MQ=255)                                                                                                                    tAACAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGaaa                                                              <  2:95592/81‑1 (MQ=255)                                                                                                                      aCAGTGCGAATACCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACccgccg  <  2:532267/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                               TTAAATCCCAGGTTGACGTCAGCTCGCCCTGCTGTTCCCGTAATTGATTGGAAACCACAAAGCTCTTCTGGCTATGGTGAAGGGAAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATA‑CCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCG  >  NC_000913/1972514‑1972768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑