Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,600,609 | (C)5→6 | coding (132/471 nt) | bcp → | peroxiredoxin, thiol peroxidase, thioredoxin‑dependent |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,600,604 | 1 | . | C | 97.0% | 110.7 / ‑3.0 | 33 | coding (127/471 nt) | bcp | peroxiredoxin, thiol peroxidase, thioredoxin‑dependent |
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (10/22); total (10/23) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 |
TGGAGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAG > NC_000913/2600466‑2600732 | tGGAGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGa > 1:265164/1‑139 (MQ=255) aGTAATGAATCCACTGAAAGCCGGTGATATCGCGCCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cccgg > 1:54694/1‑136 (MQ=255) aGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cccgg < 1:53430/139‑4 (MQ=255) tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTa < 1:200909/139‑1 (MQ=255) aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACa > 1:770341/1‑139 (MQ=255) cTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cccgg > 2:77095/1‑123 (MQ=255) cTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cccgg < 1:77095/126‑4 (MQ=255) gTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCt < 1:39231/139‑1 (MQ=255) gTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCt < 1:773742/139‑1 (MQ=255) tGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCtt > 1:778581/1‑139 (MQ=255) gATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTa < 2:578114/139‑1 (MQ=255) gATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTa < 1:798351/139‑1 (MQ=255) cGCACCGAAATTTAGCTTGCTGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCATGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa < 2:99823/139‑1 (MQ=255) cGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa < 2:265164/139‑1 (MQ=255) aaaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATg > 2:530982/1‑139 (MQ=255) aaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATgg < 1:673825/139‑1 (MQ=255) tttAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCCTCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt < 2:785301/139‑1 (MQ=255) aGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCggg < 1:530982/139‑1 (MQ=255) gagaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTg < 2:54694/139‑1 (MQ=255) agaATAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGTAAGCCATGA‑ccc‑cccgg < 2:636520/76‑4 (MQ=255) agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cccgg > 1:636520/1‑73 (MQ=255) gaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAt < 2:928107/139‑1 (MQ=255) aaCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtg < 1:910880/139‑1 (MQ=255) aaCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtg < 2:952257/139‑1 (MQ=255) gTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTg < 2:440805/139‑1 (MQ=255) tttGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTAt < 1:996003/139‑1 (MQ=255) gACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAg < 2:778581/139‑1 (MQ=255) gACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAg > 1:928450/1‑139 (MQ=255) cGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTg > 1:247434/1‑120 (MQ=255) cGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTg < 2:247434/120‑1 (MQ=255) ccAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATaa < 1:941770/139‑1 (MQ=255) cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTa > 2:107241/1‑47 (MQ=255) cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTa < 1:107241/47‑1 (MQ=255) cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtg > 2:289520/1‑107 (MQ=255) cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtg < 1:289520/107‑1 (MQ=255) actcGAAAGCCATGACCCC‑CCGTCTGTACCTTACAGGCCTGGGGCTTACGCGATAACATGGATGAGTTGAACAAAGCGGGCGTTGCTGTGGTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGaaa > 2:1069936/4‑139 (MQ=255) aCCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGaaa > 2:133111/1‑139 (MQ=255) aCCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGaaa > 1:574992/1‑139 (MQ=255) aaaGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAagag < 2:917747/139‑1 (MQ=255) aaaGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAagag < 1:133111/139‑1 (MQ=255) | TGGAGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAG > NC_000913/2600466‑2600732 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |