| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,905,219 | (C)6→7 | coding (200/672 nt) | queE ← | 7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,905,213 | 1 | . | C | 100.0% | 171.6 / NA | 48 | A69G (GCT→GGT) | queE | 7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (24/24); total (24/24) | |||||||||||
AGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAA > NC_000913/2905076‑2905345 | aGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑c < 1:161340/139‑1 (MQ=255)aGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑c < 2:1040510/139‑1 (MQ=255) aaaaCCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑ccccc < 1:173303/139‑1 (MQ=255) cTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATc > 2:22943/1‑139 (MQ=255) ttCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAct < 1:377846/139‑1 (MQ=255) ttCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAct < 1:59424/139‑1 (MQ=255) tcagtcagTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTc < 1:432419/139‑1 (MQ=255) ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCTAATCTTCACTGCTCGCAGCCCCCCCACTTATCGCTCGCTTTGGTCTTCGCCAGa < 2:746121/139‑1 (MQ=255) ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAc > 1:256903/1‑119 (MQ=255) ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAc < 2:256903/119‑1 (MQ=255) aTGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGgag < 1:988553/139‑1 (MQ=255) tGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCtt < 1:693198/116‑1 (MQ=255) ggCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCtt > 2:693198/2‑116 (MQ=255) gCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑ccc > 1:945256/1‑84 (MQ=255) gCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑ccc < 2:945256/84‑1 (MQ=255) caccacCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAg < 2:935267/78‑1 (MQ=255) caccacCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAg > 1:935267/1‑78 (MQ=255) accCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTc < 2:930521/139‑1 (MQ=255) ccGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCaa > 1:437321/1‑139 (MQ=255) tAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGgaga > 2:93276/1‑121 (MQ=255) tAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGgaga < 1:93276/121‑1 (MQ=255) tAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCt < 2:437321/139‑1 (MQ=255) aTGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgt > 2:761049/1‑139 (MQ=255) gCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtgt > 2:208842/1‑139 (MQ=255) cgcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTg > 2:955181/1‑79 (MQ=255) cgcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTg < 1:955181/79‑1 (MQ=255) cgcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtgtgt > 1:351236/1‑139 (MQ=255) gcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGtt < 1:727054/139‑1 (MQ=255) cgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGttt > 2:317940/1‑139 (MQ=255) cccTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCAc < 1:317940/139‑1 (MQ=255) gCGACCAATGACAGTCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTg < 2:758909/80‑1 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAc < 2:757652/54‑1 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAc > 1:757652/1‑54 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAAt < 2:59490/76‑1 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAAt > 1:59490/1‑76 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTg > 1:758909/1‑80 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTcc < 2:1072989/113‑1 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTcc > 1:1072989/1‑113 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgt > 2:151951/1‑118 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgt < 1:151951/118‑1 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc > 1:746248/1‑139 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc > 2:142327/1‑139 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc > 2:519015/1‑139 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc > 2:631304/1‑139 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc > 2:632863/1‑139 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc > 2:694106/1‑139 (MQ=255) tGACAGCCAGCAAAGCTTCACTGGTCGCAGCCCCCCCACTTATCTCTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCGAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCaa < 2:619233/139‑1 (MQ=255) gccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCggg < 1:631304/139‑1 (MQ=255) ccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGgag < 1:541197/75‑1 (MQ=255) ccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGgag > 2:541197/1‑75 (MQ=255) cagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCa < 1:761049/139‑1 (MQ=255) agcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCAt < 1:22943/139‑1 (MQ=255) cTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAg > 2:88251/1‑75 (MQ=255) cTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAg < 1:88251/75‑1 (MQ=255) tCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATaa > 2:25990/1‑139 (MQ=255) | AGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAA > NC_000913/2905076‑2905345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |