Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,984	(G)7→8	coding (1041/1245 nt)	sstT →	sodium:serine/threonine symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,977	1	.	G	100.0%	138.6 / NA	39	A345G (GCG→GGG)	sstT	sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/21);  total (18/21)

GCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAAC  >  NC_000913/3240844‑3241107                                                                                                                                       |                                                                                                                                   gcgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggg                                                                                                                               >  2:548739/1‑139 (MQ=255)   gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggg                                                                                                                             <  2:499419/139‑1 (MQ=255)     gcgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           <  1:901741/139‑3 (MQ=255)     gcgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           >  1:486085/1‑137 (MQ=255)         agcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTctct                                                                                                                        <  1:548739/139‑1 (MQ=255)          gcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCAGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctg                                                                                                                       <  2:886/139‑1 (MQ=255)                     tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATccc                                                                                                            >  1:298499/1‑139 (MQ=255)                        cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                                                         <  2:486085/139‑1 (MQ=255)                          tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                                                         >  1:315844/1‑137 (MQ=255)                          tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                                                         <  2:315844/137‑1 (MQ=255)                          tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                       >  2:327827/1‑139 (MQ=255)                          tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                       >  2:336503/1‑139 (MQ=255)                            ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGcc                                                                                                     >  2:61292/1‑139 (MQ=255)                            ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTAGcc                                                                                                     >  2:405917/1‑139 (MQ=255)                            ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           >  2:223905/1‑114 (MQ=255)                            ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           <  1:223905/116‑3 (MQ=255)                                cGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                                                 <  1:336503/139‑1 (MQ=255)                                    ggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑gggg                                                                                                                                <  1:635508/103‑1 (MQ=255)                                    ggctgCGGTTAATACGCAGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑gggg                                                                                                                                >  2:635508/1‑103 (MQ=255)                                       tgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGtt                                                                                          <  1:327827/139‑1 (MQ=255)                                         cGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      >  2:42924/1‑125 (MQ=255)                                         cGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      <  1:42924/125‑1 (MQ=255)                                           gTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           >  2:994119/1‑99 (MQ=255)                                           gTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                           <  1:994119/101‑3 (MQ=255)                                                     tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      >  2:124698/1‑113 (MQ=255)                                                     tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      <  1:124698/113‑1 (MQ=255)                                                      gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           >  2:590767/1‑139 (MQ=255)                                                      gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           >  2:452909/1‑139 (MQ=255)                                                      gggTATTCCGGCCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           <  1:722071/139‑1 (MQ=255)                                                           ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g                                                                                                                                   >  1:941082/1‑77 (MQ=255)                                                           ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g                                                                                                                                   <  2:941082/77‑1 (MQ=255)                                                                   gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                                                              >  2:213847/1‑139 (MQ=255)                                                                               gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      >  2:47112/1‑87 (MQ=255)                                                                               gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                      <  1:47112/87‑1 (MQ=255)                                                                                   tgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTc                                                                                         <  1:350097/96‑1 (MQ=255)                                                                                   tgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTc                                                                                         >  2:350097/1‑96 (MQ=255)                                                                                     ctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCttt                                            >  2:482355/1‑139 (MQ=255)                                                                                                  tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           <  2:771664/95‑1 (MQ=255)                                                                                                  tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           >  1:771664/1‑95 (MQ=255)                                                                                                      ttCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCa                           <  1:55960/139‑1 (MQ=255)                                                                                                          ctGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGAc                       <  2:915474/139‑1 (MQ=255)                                                                                                                     tgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           <  2:165658/76‑1 (MQ=255)                                                                                                                     tgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           >  1:165658/1‑76 (MQ=255)                                                                                                                      ggCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAAcc           <  2:461947/139‑1 (MQ=255)                                                                                                                       gcgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                                                         >  2:44500/1‑44 (MQ=38)                                                                                                                       gcgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                                                         <  1:44500/44‑1 (MQ=255)                                                                                                                       gcgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGt                                                                                                  >  1:713908/1‑51 (MQ=255)                                                                                                                       gcgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGt                                                                                                  <  2:713908/51‑1 (MQ=255)                                                                                                                            tCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCTGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTg     <  1:590767/139‑1 (MQ=255)                                                                                                                            tCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTg     <  1:452909/139‑1 (MQ=255)                                                                                                                               ggCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAAc  <  1:213847/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   GCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAAC  >  NC_000913/3240844‑3241107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑