Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,746	(A)8→9	coding (599/906 nt)	yhfZ ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,738	1	.	A	100.0%	157.5 / NA	49	G203C (GGC→TGC)	yhfZ	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (24/25);  total (24/25)

CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC  >  NC_000913/3511604‑3511871                                                                                                                                        |                                                                                                                                      cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaa                                                                                                                                   >  2:432191/1‑139 (MQ=255) cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaa                                                                                                                                   >  1:193616/1‑139 (MQ=255)             tCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGt                                                                                                                        <  1:432191/139‑1 (MQ=255)                 aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaaaaaaaatggttcaga                                                                                                                   >  2:116419/1‑127 (MQ=255)                            ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATc                                                                                                         >  1:303294/1‑139 (MQ=255)                                    ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAAc                                                                                                 <  2:978295/139‑1 (MQ=255)                                                 ccAGATCACCGCATCGACATCGCCTTTAACAATTCGTTGTAAACTCTCTTGATAAGAGGGATCGACTCGTTCCACATCACTACCGCC‑aaaaaaaaaca                                                                                                                            <  2:111984/98‑4 (MQ=255)                                                 ccAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaaaaaaca                                                                                                                            >  1:111984/1‑95 (MQ=255)                                                     aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                <  2:222785/139‑1 (MQ=255)                                                     aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                >  1:119721/1‑139 (MQ=255)                                                        aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCTACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACgcgc                                                                             >  2:1046970/1‑139 (MQ=255)                                                         ccGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATc                                                                                          <  1:500856/125‑1 (MQ=255)                                                         ccGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATAGGTCATGATTTTCTGATCCGCCGAACGGTTATc                                                                                          >  2:500856/1‑125 (MQ=255)                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                >  1:248972/1‑134 (MQ=255)                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                <  2:248972/134‑1 (MQ=255)                                                                  cATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTg                                                                   <  1:363945/139‑1 (MQ=255)                                                                                        tAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                      >  2:439216/1‑66 (MQ=255)                                                                                        tAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                      <  1:439216/66‑1 (MQ=255)                                                                                               tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            >  2:861723/1‑117 (MQ=255)                                                                                               tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            <  1:861723/117‑1 (MQ=255)                                                                                                    aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                                 >  1:71439/1‑139 (MQ=255)                                                                                                    aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                                 <  2:580077/139‑1 (MQ=255)                                                                                                    aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                                 <  2:193616/139‑1 (MQ=255)                                                                                                       agagagATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                      <  1:537541/51‑1 (MQ=255)                                                                                                       agagagATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                      >  2:537541/1‑51 (MQ=255)                                                                                                       agagagATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                    >  2:421889/1‑133 (MQ=255)                                                                                                       agagagATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                    <  1:421889/133‑1 (MQ=255)                                                                                                         agagaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               >  2:404286/1‑72 (MQ=255)                                                                                                         agagaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               <  1:404286/72‑1 (MQ=255)                                                                                                           agaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACg                          <  2:71439/139‑1 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    <  1:514928/63‑1 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    <  2:366535/63‑1 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    <  2:461195/63‑1 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    >  1:366535/1‑63 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    >  2:514928/1‑63 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    >  1:461195/1‑63 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                 <  2:87240/114‑1 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                 >  1:87240/1‑114 (MQ=255)                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa                        >  1:1011615/1‑139 (MQ=255)                                                                                                                 aCTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGgtg                    >  1:100491/1‑139 (MQ=255)                                                                                                                   tCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg                  <  2:119721/139‑1 (MQ=255)                                                                                                                     gTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                                 >  1:87276/1‑90 (MQ=255)                                                                                                                     gTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                                 <  2:87276/90‑1 (MQ=255)                                                                                                                     gTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg                <  2:303294/139‑1 (MQ=255)                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    >  2:134287/1‑43 (MQ=255)                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    <  1:134287/43‑1 (MQ=255)                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCg                                <  2:187153/111‑1 (MQ=255)                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCg                                >  1:187153/1‑111 (MQ=255)                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa    >  2:888502/1‑139 (MQ=255)                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    >  2:700274/1‑41 (MQ=255)                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    <  1:700274/41‑1 (MQ=255)                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               >  2:472603/1‑46 (MQ=255)                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               <  1:472603/46‑1 (MQ=255)                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCGCCAACTCCAgc  >  2:520999/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                      CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC  >  NC_000913/3511604‑3511871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑