| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,709,561 | (C)6→7 | coding (915/1692 nt) | eptB ← | KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,709,555 | 1 | . | C | 97.6% | 133.4 / ‑3.2 | 41 | coding (921/1692 nt) | eptB | KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (17/23); total (17/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01 | |||||||||||
CAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑G‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACC > NC_000913/3709419‑3709686 | cAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑G‑cc < 1:391846/139‑1 (MQ=255) tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑G‑ccccccctga > 2:166290/1‑135 (MQ=255) tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑G‑ccccccctga > 1:536367/1‑135 (MQ=255) cGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACg < 1:259131/139‑1 (MQ=255) tCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACaa > 1:821399/1‑139 (MQ=255) gtgtTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAAc < 1:466531/139‑1 (MQ=255) gTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCAC‑CCCCCCTGACGTACAAACATGCAACg < 1:602600/139‑1 (MQ=255) gTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGc < 1:731702/135‑1 (MQ=255) gTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGATTCAGAACCGCGAAAATGTTCTGTTCTTTGAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGc > 2:731702/1‑135 (MQ=255) aaCCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGt < 2:377214/139‑1 (MQ=255) caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgc‑g‑c < 2:344592/104‑1 (MQ=255) caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgc‑g‑c > 1:344592/1‑104 (MQ=255) ctctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGtt < 1:785679/139‑1 (MQ=255) gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg < 1:445898/139‑1 (MQ=255) gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg < 2:1082785/139‑1 (MQ=255) gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg < 1:440280/139‑1 (MQ=255) cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac > 2:733726/1‑139 (MQ=255) aGAACTGAATCCTAATTGCTTCAGAACCGCGAAAATATTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCACCGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGCAAcc > 2:341367/1‑139 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACg < 2:586166/103‑1 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc > 2:273865/1‑139 (MQ=255) aGAACTGAATCCTAACTGATTCAGAACCGCGAAAATGTTCTGTGCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACg > 1:586166/1‑103 (MQ=255) aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgc‑g‑c < 2:1109417/70‑1 (MQ=255) aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgc‑g‑c > 1:1109417/1‑70 (MQ=255) ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgc‑g‑c < 1:926024/58‑1 (MQ=255) ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgc‑g‑c > 2:926024/1‑58 (MQ=255) gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt > 2:435643/1‑95 (MQ=255) gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt < 1:435643/95‑1 (MQ=255) ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGAGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCt < 2:1112902/139‑1 (MQ=255) cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca < 1:260374/99‑1 (MQ=255) cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca > 2:260374/1‑99 (MQ=255) gttctgttctTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGttt < 1:643017/139‑1 (MQ=255) ttctgttctTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTcc > 1:580757/1‑127 (MQ=255) ttctgttctTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTcc < 2:580757/127‑1 (MQ=255) gttctTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg < 2:821399/139‑1 (MQ=255) gttctTTTAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg < 2:536367/139‑1 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc < 2:242767/74‑1 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc > 1:242767/1‑74 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTAt > 2:1044315/1‑139 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTAt > 1:370653/1‑139 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGC‑G‑ccccccc > 1:777690/1‑35 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGC‑G‑ccccccc < 2:777690/36‑2 (MQ=255) gtgCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCaa > 1:766120/1‑49 (MQ=255) gtgCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCaa < 2:766120/49‑1 (MQ=255) gtgCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg < 2:585661/71‑1 (MQ=255) gtgCGCTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg > 1:585661/1‑71 (MQ=255) cgcTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg > 2:589891/1‑68 (MQ=255) cgcTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg < 1:589891/68‑1 (MQ=255) cgcTGCGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa > 1:986916/1‑139 (MQ=255) cGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAAc > 2:507949/1‑42 (MQ=255) cGGATTATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAAc < 1:507949/42‑1 (MQ=255) ttATCTTCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaaa < 2:370653/139‑1 (MQ=255) ttCCGC‑GCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAcc < 2:986916/139‑1 (MQ=255) | CAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑G‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACC > NC_000913/3709419‑3709686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |