| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,606,330 | (G)8→9 | noncoding (72/87 nt) | leuQ ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,606,322 | 1 | . | G | 86.1% | 99.3 / 11.0 | 36 | noncoding (80/87 nt) | leuQ | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (3/2); new base G (16/15); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
TTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTC > NC_000913/4606184‑4606446 | ttGATATCGCCTCGTTTGGTGGGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa < 1:584977/139‑1 (MQ=255)ttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCTAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa > 1:713687/1‑139 (MQ=255)ttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa > 1:137611/1‑139 (MQ=255) tttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggg‑ggggggcttg > 1:155310/1‑133 (MQ=255) gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGt > 2:520528/1‑139 (MQ=255) gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGCGGGGTCTTGAACCCCCACGTCCGt > 1:216414/1‑139 (MQ=255) tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGTGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGac < 2:713687/139‑1 (MQ=255) gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAAcccc < 1:179664/123‑1 (MQ=255) gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAAcccc > 2:179664/1‑123 (MQ=255) gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggg‑gggggac > 1:765370/1‑111 (MQ=255) gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggg‑gggggac < 2:765370/114‑4 (MQ=255) gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAACAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGTCTTGAACCCCCACGTCCGTAAGTaca > 2:926571/1‑139 (MQ=21) aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACt < 1:520528/139‑1 (MQ=255) ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca > 1:711668/1‑139 (MQ=255) gacacTAACTCCTGAAGCTAGCGCCTCTACCAATTCCGCAACCGACGCCCAGTGCGATTATTAGAAACGGGGTTTTTGGGGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg < 2:137611/138‑1 (MQ=18) acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCa > 1:797768/1‑103 (MQ=255) acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCa < 2:797768/103‑1 (MQ=255) ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt > 2:86770/1‑139 (MQ=255) aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg < 1:548432/139‑1 (MQ=255) cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt > 2:739844/1‑139 (MQ=255) tCTACCAATTCCGCCACCTTCGCCCAGTGCGATCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGTCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc > 2:548432/1‑139 (MQ=255) tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca > 1:946561/1‑101 (MQ=255) tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca < 2:946561/101‑1 (MQ=255) tcctaTTCCGCCACGTTCGCTCAGTGCGAGAAACATCAACGGCGTTGTTGGGGGGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATa < 2:155310/135‑1 (MQ=14) ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTc > 1:235304/1‑139 (MQ=255) cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc > 1:204193/1‑125 (MQ=255) cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc < 2:204193/125‑1 (MQ=255) tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACg > 2:957937/1‑56 (MQ=25) tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACg < 1:957937/56‑1 (MQ=25) aGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACg < 2:526113/52‑1 (MQ=25) aGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACg > 1:526113/1‑52 (MQ=25) aGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa < 2:1088970/118‑1 (MQ=255) aGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa > 1:1088970/1‑118 (MQ=255) aaTATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg < 1:739844/139‑1 (MQ=255) atCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg > 2:1069315/1‑139 (MQ=255) aaCGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg < 2:235304/139‑1 (MQ=255) gTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg > 2:504619/1‑69 (MQ=255) gTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg < 1:504619/69‑1 (MQ=255) tGGTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTc < 1:86770/139‑1 (MQ=255) gTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa < 1:949784/103‑1 (MQ=255) gTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa > 2:949784/1‑103 (MQ=255) gTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt > 1:83482/1‑116 (MQ=255) gTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt < 2:83482/116‑1 (MQ=255) | TTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTC > NC_000913/4606184‑4606446 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |