Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 49,767 A→G intergenic (+136/‑56) kefC → / → folA potassium:proton antiporter/dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091349,7670AG100.0% 193.6 / NA 54intergenic (+136/‑56)kefC/folApotassium:proton antiporter/dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/28);  total (26/28)

AGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTG  >  NC_000913/49637‑49894
                                                                                                                                  |                                                                                                                               
agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                         >  1:1109988/1‑139 (MQ=255)
agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                         >  2:958791/1‑139 (MQ=255)
 gTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGttt                                                                                                                        >  2:347932/1‑139 (MQ=255)
    aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACg                                                                                                                     >  2:664989/1‑139 (MQ=255)
     cGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    <  2:1109988/139‑1 (MQ=255)
     cGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    <  2:377001/139‑1 (MQ=255)
      gTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATTCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCAAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   <  1:818285/139‑1 (MQ=255)
      gTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   <  1:87946/139‑1 (MQ=255)
      gTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   <  1:664989/139‑1 (MQ=255)
                   tACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAGCATCATCCTCGCACCAGTTGGCGACGGttt                                                                                                                        <  2:410072/121‑1 (MQ=255)
                   tACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGttt                                                                                                                        >  1:410072/1‑121 (MQ=255)
                      ccgcattacaatgtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGa                                                                                                   >  1:375625/12‑139 (MQ=255)
                         tAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACaa                                                                                                >  2:19330/1‑139 (MQ=255)
                         tAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACaa                                                                                                >  1:91126/1‑139 (MQ=255)
                           aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    <  2:457019/117‑1 (MQ=255)
                           aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    >  1:457019/1‑117 (MQ=255)
                             tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   <  2:761754/116‑1 (MQ=255)
                             tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   >  1:761754/1‑116 (MQ=255)
                               tttgtttttgttTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAtttttt                                                                                          >  1:192256/1‑139 (MQ=255)
                                       tgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCttt                                                                                                                 <  2:819274/108‑1 (MQ=255)
                                       tgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCttt                                                                                                                 >  1:819274/1‑108 (MQ=255)
                                        gtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                 <  2:192256/139‑1 (MQ=255)
                                                   aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc                                                                                                                      <  2:599524/91‑1 (MQ=255)
                                                   aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc                                                                                                                      >  1:599524/1‑91 (MQ=255)
                                                   aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCttt                                                                                                                 <  2:615398/96‑1 (MQ=255)
                                                   aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCttt                                                                                                                 >  1:615398/1‑96 (MQ=255)
                                                    tGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt                                                                     <  1:347932/139‑1 (MQ=255)
                                                      cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc                                                                                                                      >  1:915024/1‑88 (MQ=255)
                                                      cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc                                                                                                                      <  2:915024/88‑1 (MQ=255)
                                                        gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                                                                 >  1:1033788/1‑139 (MQ=255)
                                                         cgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTc                                                                <  1:221092/139‑1 (MQ=255)
                                                                     gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                    <  1:958791/139‑1 (MQ=255)
                                                                      agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtctc                                                                           >  2:381492/1‑115 (MQ=255)
                                                                      agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtctc                                                                           <  1:381492/115‑1 (MQ=255)
                                                                       gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   <  2:58802/74‑1 (MQ=255)
                                                                       gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   >  1:58802/1‑74 (MQ=255)
                                                                       gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                    >  1:789313/1‑137 (MQ=255)
                                                                       gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                    <  2:789313/137‑1 (MQ=255)
                                                                         gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc                                                <  2:912419/139‑1 (MQ=255)
                                                                                cTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGAt                                         <  2:375625/139‑1 (MQ=255)
                                                                                        cagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt                                                                     >  1:170653/1‑103 (MQ=255)
                                                                                        cagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt                                                                     <  2:170653/103‑1 (MQ=255)
                                                                                                       tCCTCAACATCATCCTCGCACCAGTTGGCGACGGttt                                                                                                                        >  2:962170/1‑37 (MQ=255)
                                                                                                       tCCTCAACATCATCCTCGCACCAGTTGGCGACGGttt                                                                                                                        <  1:962170/37‑1 (MQ=255)
                                                                                                                 catcCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTgg        >  1:8797/1‑139 (MQ=255)
                                                                                                                   tcCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCg           >  2:607366/1‑134 (MQ=255)
                                                                                                                   tcCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCg           <  1:607366/134‑1 (MQ=255)
                                                                                                                      tCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcct   >  1:525991/1‑139 (MQ=255)
                                                                                                                      tCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcct   <  2:8797/139‑1 (MQ=255)
                                                                                                                       cGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctg  <  1:290492/139‑1 (MQ=255)
                                                                                                                         cACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa                                                                               <  1:49969/60‑1 (MQ=255)
                                                                                                                         cACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa                                                                               >  2:49969/1‑60 (MQ=255)
                                                                                                                         cACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                 <  1:28386/58‑1 (MQ=255)
                                                                                                                         cACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                 >  2:28386/1‑58 (MQ=255)
                                                                                                                                  |                                                                                                                               
AGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTG  >  NC_000913/49637‑49894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: