Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	477,025	G→A	*118* (TAG→TAA)	ybaA →	DUF1428 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	477,025	0	G	A	100.0%	122.1 / NA	39	*118* (TAG→TAA)	ybaA	DUF1428 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/20);  total (19/20)

CCTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGG  >  NC_000913/476891‑477157                                                                                                                                       |                                                                                                                                     ccTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAg                                                                                                                                  >  1:995536/1‑139 (MQ=255)          gagGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACggg                                                                                                                         <  1:485017/139‑1 (MQ=255)           agGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGc                                                                                                                        >  1:466720/1‑139 (MQ=255)               ccGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCtgtg                                                                                                                    >  2:535054/1‑139 (MQ=255)                    aCGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTgcggcg                                                                                                               <  1:1111083/139‑1 (MQ=255)                          ctAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGt                                                                                                         <  1:1074146/139‑1 (MQ=255)                          ctAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGt                                                                                                         <  1:535054/139‑1 (MQ=255)                                    gatgatGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaaaa                                                                                               >  1:103966/1‑139 (MQ=255)                                       gatgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAAtt                                                                                            >  1:155474/1‑139 (MQ=255)                                       gatgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAAtt                                                                                            >  1:734824/1‑139 (MQ=255)                                                    cGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                               <  2:849898/139‑1 (MQ=255)                                                         gAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGcc                                                                          >  1:608574/1‑139 (MQ=255)                                                            agagTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAg                                                                       <  2:608574/139‑1 (MQ=255)                                                                ttCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTa                                                                   >  1:324006/1‑139 (MQ=255)                                                                 tCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGTGGATTCGAGTCAATCATCGACGAATAAGTAGCGTg                                                                                                                              >  1:455210/1‑79 (MQ=255)                                                                 tCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGTGGATTCGAGTCAATCATCGACGAATAAGTAGCGTg                                                                                                                              <  2:455210/79‑1 (MQ=255)                                                                                     gACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATg                                              <  2:466720/139‑1 (MQ=255)                                                                                       cGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGcc                                            <  1:401987/139‑1 (MQ=255)                                                                                          cAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTg                                         >  2:500777/1‑139 (MQ=255)                                                                                                   gATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGagc                                <  2:734824/139‑1 (MQ=255)                                                                                                       tATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGagccagc                            >  2:850778/1‑139 (MQ=255)                                                                                                              gATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGAcc                     <  2:285685/139‑1 (MQ=255)                                                                                                              gATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGAcc                     >  2:632341/1‑139 (MQ=255)                                                                                                               aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCt                                                                                                                       <  1:210824/40‑1 (MQ=38)                                                                                                               aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCt                                                                                                                       >  2:210824/1‑40 (MQ=38)                                                                                                               aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGcc                                                                          >  1:286559/1‑85 (MQ=255)                                                                                                               aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGcc                                                                          <  2:286559/85‑1 (MQ=255)                                                                                                               aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAg                                                                 <  2:521517/94‑1 (MQ=255)                                                                                                               aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAg                                                                 >  1:521517/1‑94 (MQ=255)                                                                                                               aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg                    >  2:971069/1‑139 (MQ=255)                                                                                                                ttCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGcc                                                                          >  1:868622/1‑84 (MQ=255)                                                                                                                ttCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGcc                                                                          <  2:868622/84‑1 (MQ=255)                                                                                                                 tCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTa                  >  1:287984/1‑139 (MQ=255)                                                                                                                   gAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGt                <  2:324006/139‑1 (MQ=255)                                                                                                                        aaTCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACg           <  1:971069/139‑1 (MQ=255)                                                                                                                             tcGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATg      <  1:850778/139‑1 (MQ=255)                                                                                                                             tcGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATg      <  2:995536/139‑1 (MQ=255)                                                                                                                               gacgaATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCt    >  2:254834/1‑139 (MQ=255)                                                                                                                                 cgaATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTgg  <  1:632341/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CCTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGG  >  NC_000913/476891‑477157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑