Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	478,533	C→T	A29T (GCC→ACC)	ylaB ←	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	478,533	0	C	T	100.0%	219.9 / NA	68	A29T (GCC→ACC)	ylaB	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/38);  total (30/38)

GCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATT  >  NC_000913/478400‑478661                                                                                                                                      |                                                                                                                                 gCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgtcagcc                                                                                                                             <  2:576589/139‑1 (MQ=255)          tGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTa                                                                                                                    <  2:152460/139‑1 (MQ=255)                cTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGa                                                                                                              <  2:908545/139‑1 (MQ=255)                       gCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                       <  2:326886/139‑1 (MQ=255)                        cTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcagc                                                                                                      <  2:290847/139‑1 (MQ=255)                          ccTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCAACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCaa                                                                                                    <  2:788164/139‑1 (MQ=255)                           cTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAAt                                                                                                   >  1:713560/1‑139 (MQ=255)                            tGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATa                                                                                                  >  2:957371/1‑139 (MQ=255)                             gCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATAc                                                                                                 >  2:1097689/1‑139 (MQ=255)                                 cgcATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa                                                                                             <  1:1074205/139‑1 (MQ=255)                                   cATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAg                                                                                           >  2:298223/1‑139 (MQ=255)                                                tgtCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTccc                                                                              >  1:865564/1‑139 (MQ=255)                                                         cTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAgg                                                                     <  1:716282/139‑1 (MQ=255)                                                                  tCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGa                                                            >  2:57717/1‑139 (MQ=255)                                                                   cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa                                                                                          <  2:667832/108‑1 (MQ=255)                                                                   cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa                                                                                          >  1:667832/1‑108 (MQ=255)                                                                   cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGAt                                                           >  2:206321/1‑139 (MQ=255)                                                                     aaTAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAgg                                                                     >  1:1063100/1‑127 (MQ=255)                                                                     aaTAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAgg                                                                     <  2:1063100/127‑1 (MQ=255)                                                                      aTAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTc                                                        <  2:835600/139‑1 (MQ=255)                                                                         gCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCgtg                                                     <  1:1097689/139‑1 (MQ=255)                                                                               cgGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                               >  2:757250/1‑139 (MQ=255)                                                                                     gaATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCa                                         <  1:1103050/139‑1 (MQ=255)                                                                                       aTAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCata                                       <  1:154302/139‑1 (MQ=255)                                                                                         aGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                     <  2:559288/139‑1 (MQ=255)                                                                                         aGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                     <  1:206321/139‑1 (MQ=255)                                                                                          ggTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATcc                                    >  2:576229/1‑139 (MQ=255)                                                                                             aTCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCtta                                 >  2:546632/1‑139 (MQ=255)                                                                                              tCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttat                                <  2:865564/139‑1 (MQ=255)                                                                                              tCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttat                                >  2:402774/1‑139 (MQ=255)                                                                                               ccAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAg                                                             <  1:758357/109‑1 (MQ=255)                                                                                               ccAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAg                                                             >  2:758357/1‑109 (MQ=255)                                                                                                cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttatta                              >  2:569949/1‑139 (MQ=255)                                                                                                  gCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAac                            <  2:713560/139‑1 (MQ=255)                                                                                                  gCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAac                            <  1:514755/139‑1 (MQ=255)                                                                                                   ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                           >  2:605751/1‑139 (MQ=255)                                                                                                   ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                           <  1:605751/139‑1 (MQ=255)                                                                                                   ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                           <  1:546632/139‑1 (MQ=255)                                                                                                   ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                           <  2:102665/139‑1 (MQ=255)                                                                                                   ctattaaaTATACGATGTTTCTACCTGCTGATGGGTCAGCTAGATGCGTAAACCGACAGGCATCAATACTGAAAGAATCCGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                           <  2:856112/133‑1 (MQ=255)                                                                                                    tctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAacac                          <  1:569949/139‑1 (MQ=255)                                                                                                      ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                           >  1:260029/1‑136 (MQ=255)                                                                                                      ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                           <  2:260029/136‑1 (MQ=255)                                                                                                      ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAg                        <  1:57717/139‑1 (MQ=255)                                                                                                      ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAg                        >  2:250330/1‑139 (MQ=255)                                                                                                        cAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                               <  1:873116/114‑1 (MQ=255)                                                                                                        cAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                               >  2:873116/1‑114 (MQ=255)                                                                                                            aaaCGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAgg                  <  1:957371/139‑1 (MQ=255)                                                                                                             aaCGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAggg                 >  2:769442/1‑139 (MQ=255)                                                                                                              aCGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                <  1:771420/139‑1 (MQ=255)                                                                                                              aCGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                <  1:298223/139‑1 (MQ=255)                                                                                                                gATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCgtg                                                     <  2:771571/100‑1 (MQ=255)                                                                                                                gATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCgtg                                                     >  1:771571/1‑100 (MQ=255)                                                                                                                gATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                <  2:542550/137‑1 (MQ=255)                                                                                                                gATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                >  1:542550/1‑137 (MQ=255)                                                                                                                  tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAACGTAGGGaaact            >  1:776057/1‑137 (MQ=255)                                                                                                                            tgctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAg                                                                      >  2:280224/1‑71 (MQ=255)                                                                                                                            tgctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAg                                                                      <  1:280224/71‑1 (MQ=255)                                                                                                                            tgctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGAtt  >  2:481985/1‑139 (MQ=255)                                                                                                                              ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCaa                                                                                                    >  2:747639/1‑39 (MQ=255)                                                                                                                              ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCaa                                                                                                    <  1:747639/39‑1 (MQ=255)                                                                                                                              ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTcc                                                                               >  2:308911/1‑60 (MQ=255)                                                                                                                              ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTcc                                                                               <  1:308911/60‑1 (MQ=255)                                                                                                                                gATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                               >  1:943867/1‑90 (MQ=255)                                                                                                                                gATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                               <  2:943867/90‑1 (MQ=255)                                                                                                                                 aTGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                           <  1:161305/109‑1 (MQ=255)                                                                                                                                 aTGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                           >  2:161305/1‑109 (MQ=255)                                                                                                                                 aTGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAg                        >  2:259234/1‑112 (MQ=255)                                                                                                                                 aTGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAg                        <  1:259234/112‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 GCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATT  >  NC_000913/478400‑478661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑