Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 66,447 T→C E35G (GAG→GGG)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091366,4470TC100.0% 185.3 / NA 53E35G (GAG→GGG) araDL‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)

GGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTT  >  NC_000913/66318‑66577
                                                                                                                                 |                                                                                                                                   
ggCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCaa                                                                                                                            >  1:679620/1‑139 (MQ=255)
          aggaggGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGAc                                                                                                                  <  1:946180/139‑1 (MQ=255)
               ggCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGc                                                                                                             >  1:1037958/1‑139 (MQ=255)
                gCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc                                                                                                            <  2:679620/139‑1 (MQ=255)
                gCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc                                                                                                            <  1:50444/139‑1 (MQ=255)
                         tACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAg                                                                                                   <  2:860168/139‑1 (MQ=255)
                              tCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                <  1:944987/121‑1 (MQ=255)
                              tCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                >  2:944987/1‑121 (MQ=255)
                               cAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGAc                                                                                             >  1:362096/1‑139 (MQ=255)
                                aaCCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGAcc                                                                                            >  2:575233/1‑139 (MQ=255)
                                     cTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                <  1:397827/114‑1 (MQ=255)
                                     cTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                <  1:589092/114‑1 (MQ=255)
                                     cTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                >  2:397827/1‑114 (MQ=255)
                                     cTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                >  2:589092/1‑114 (MQ=255)
                                      ttCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTtg                                                                                      >  1:349373/1‑139 (MQ=255)
                                               ttCGATGCTAGCCACGACCATATCGTCAGCGGTCATAACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGGCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATTTGAGCGTGACCAGGTTGTGTTTTGGc                                                                             <  2:986445/139‑1 (MQ=39)
                                                tCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGc                                                                                                             >  1:994195/1‑106 (MQ=255)
                                                tCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGc                                                                                                             <  2:994195/106‑1 (MQ=255)
                                                tCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTGGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCa                                                                            >  1:670872/1‑139 (MQ=255)
                                                         aCCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGt                                                                   >  1:664515/1‑139 (MQ=255)
                                                          ccACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGtt                                                                  >  1:756099/1‑139 (MQ=255)
                                                          ccACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGtt                                                                  <  2:756099/139‑1 (MQ=255)
                                                           cACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTg                                                                 <  2:882543/139‑1 (MQ=255)
                                                              gACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                >  2:937341/1‑89 (MQ=255)
                                                              gACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                <  1:937341/89‑1 (MQ=255)
                                                                   tatCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTaa                                                         >  1:766989/1‑139 (MQ=255)
                                                                     tCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATa                                                       >  2:244515/1‑139 (MQ=255)
                                                                       gTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑cc                                                    <  2:362096/139‑1 (MQ=255)
                                                                          aGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTc                                                            <  2:366669/129‑1 (MQ=255)
                                                                          aGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTc                                                            >  1:366669/1‑129 (MQ=255)
                                                                               tCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                <  2:35163/72‑1 (MQ=255)
                                                                               tCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                                >  1:35163/1‑72 (MQ=255)
                                                                               tCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGttt                                            >  1:1095097/1‑139 (MQ=255)
                                                                               tCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGttt                                            >  2:801869/1‑139 (MQ=255)
                                                                                  tGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTgag                                         <  1:575233/139‑1 (MQ=255)
                                                                                   gACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGgcg                                                                                                                       <  1:588570/61‑1 (MQ=255)
                                                                                   gACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGgcg                                                                                                                       >  2:588570/1‑61 (MQ=255)
                                                                                       cTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTgg                                                                <  2:430819/112‑1 (MQ=255)
                                                                                       cTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTgg                                                                >  1:430819/1‑112 (MQ=255)
                                                                                          tAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑cc                                                    <  2:21911/120‑1 (MQ=255)
                                                                                          tAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑cc                                                    >  1:21911/1‑120 (MQ=255)
                                                                                          tAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTa                                 <  2:664515/139‑1 (MQ=255)
                                                                                          tAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTa                                 <  2:766989/139‑1 (MQ=255)
                                                                                             tCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGGGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACa                              <  2:589009/139‑1 (MQ=255)
                                                                                                    cGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGAc                       <  1:801869/139‑1 (MQ=255)
                                                                                                    cGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGAc                       >  1:992502/1‑139 (MQ=255)
                                                                                                      gAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTc                     >  1:567771/1‑139 (MQ=255)
                                                                                                                  acaAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGa         <  2:346952/139‑1 (MQ=255)
                                                                                                                   caAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGa         <  2:428632/139‑1 (MQ=255)
                                                                                                                     aaGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATgc      >  1:280558/1‑139 (MQ=255)
                                                                                                                         cgccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCtt  >  2:870658/1‑139 (MQ=255)
                                                                                                                            cgcgCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGt                                                                                        <  2:1069461/51‑1 (MQ=255)
                                                                                                                            cgcgCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGt                                                                                        >  1:1069461/1‑51 (MQ=255)
                                                                                                                                 |                                                                                                                                   
GGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATA‑CCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTT  >  NC_000913/66318‑66577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑