Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,229	C→T	*233* (TAG→TAA)	thiQ ←	thiamine/thiamine pyrophosphate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,229	0	C	T	100.0%	125.1 / NA	39	*233* (TAG→TAA)	thiQ	thiamine/thiamine pyrophosphate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/19);  total (20/19)

AAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACG  >  NC_000913/72098‑72361                                                                                                                                    |                                                                                                                                     aaaGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACACCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:89455/139‑1 (MQ=255)   aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               >  1:695320/1‑137 (MQ=255)   aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:695320/137‑1 (MQ=255)   aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                             <  1:942292/139‑1 (MQ=255)   aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                             <  1:879620/139‑1 (MQ=255)             gTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATa                                                                                                                   >  1:387521/1‑139 (MQ=255)                      aTAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGGGTCTTGTCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCAGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAg                                                                                                          >  1:743142/1‑139 (MQ=255)                        aGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGCTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCa                                                                                                        >  2:990142/1‑139 (MQ=255)                              gtgCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:907348/110‑1 (MQ=255)                              gtgCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               >  1:907348/1‑110 (MQ=255)                                   ggTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTAcc                                                                                             >  1:592495/1‑139 (MQ=255)                                       gCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTc                                                                                         <  2:592495/139‑1 (MQ=255)                                          tGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTcaac                                                                                      <  2:802325/139‑1 (MQ=255)                                                           cgTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGCTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATAcc                                                                     <  1:990142/139‑1 (MQ=255)                                                                 aTCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCa                                                               >  2:707485/1‑139 (MQ=255)                                                                      gCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGa                                                          >  2:780951/1‑139 (MQ=255)                                                                           cAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               >  1:365118/1‑65 (MQ=255)                                                                           cAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:365118/65‑1 (MQ=255)                                                                           cAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  1:692435/112‑1 (MQ=255)                                                                           cAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                >  2:692435/1‑112 (MQ=255)                                                                                   aCTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGc                                             >  1:417762/1‑139 (MQ=255)                                                                                   aCTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGc                                             <  2:417762/139‑1 (MQ=255)                                                                                      aCCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGac                                          >  2:528396/1‑139 (MQ=255)                                                                                          gTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGactact                                      >  2:13374/1‑139 (MQ=255)                                                                                                 ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCg                                                                                                             >  2:601655/1‑61 (MQ=255)                                                                                                 ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCg                                                                                                             <  1:601655/61‑1 (MQ=255)                                                                                                 ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  2:750262/90‑1 (MQ=255)                                                                                                 ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                >  1:750262/1‑90 (MQ=255)                                                                                                 ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgc                               >  1:824167/1‑139 (MQ=255)                                                                                                 ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgc                               >  2:989023/1‑139 (MQ=255)                                                                                                      aGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTg                          <  1:13374/139‑1 (MQ=255)                                                                                                         cGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCg                       >  2:210829/1‑139 (MQ=255)                                                                                                            tCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATc                    >  1:481271/1‑139 (MQ=255)                                                                                                             cACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATcc                   >  2:679200/1‑139 (MQ=255)                                                                                                               cgccgcATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCcgc                 <  1:780951/139‑1 (MQ=255)                                                                                                                 ccgcATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCgcgc               <  2:481271/139‑1 (MQ=255)                                                                                                                    cATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGcgccgc            <  1:679200/139‑1 (MQ=255)                                                                                                                    cATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGcgccgc            <  1:210829/139‑1 (MQ=255)                                                                                                                              tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACg  <  2:824167/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     AAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACG  >  NC_000913/72098‑72361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑