Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	821,506	G→A	*238* (TAG→TAA)	ybhM →	BAX Inhibitor‑1 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	821,506	0	G	A	100.0%	183.8 / NA	59	*238* (TAG→TAA)	ybhM	BAX Inhibitor‑1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/28);  total (31/28)

AGAGTTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAG  >  NC_000913/821371‑821632                                                                                                                                        |                                                                                                                               agagTTGTGAAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCATTGGGATACTTGCCCCGATAGCTATAACGATTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCg                                                                                                                             >  2:792513/1‑139 (MQ=255)  gagTTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGt                                                                                                                            >  2:501155/1‑139 (MQ=255)        tgGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATATTTGCCGCGATAGCTATATCGCTTGTCAGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                      <  1:792513/139‑1 (MQ=255)        tgGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATg                                                                                                                       <  1:153606/138‑1 (MQ=255)        tgGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATg                                                                                                                       >  2:153606/1‑138 (MQ=255)              ccGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                      <  1:205197/133‑1 (MQ=255)              ccGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                      >  2:205197/1‑133 (MQ=255)               cGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACt                                                                                                               >  1:705553/1‑139 (MQ=255)               cGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACt                                                                                                               >  2:326669/1‑139 (MQ=255)                     gTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGaa                                                                                                         <  2:705553/139‑1 (MQ=255)                      ttGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                     <  2:990949/126‑1 (MQ=255)                      ttGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                     >  1:990949/1‑126 (MQ=255)                         cgcTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTa                                                                                                     >  1:704473/1‑139 (MQ=255)                                aCAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAAc                                                                                              >  1:793906/1‑139 (MQ=255)                                      gCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTcaca                                                                                        >  2:1078193/1‑139 (MQ=255)                                               ctctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATATGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCtt                                                                               >  2:1082203/1‑139 (MQ=255)                                               ctctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                     <  1:498504/101‑1 (MQ=255)                                               ctctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                     >  2:498504/1‑101 (MQ=255)                                                tctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTc                                                                              >  2:1059102/1‑139 (MQ=255)                                                    ttACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCAttt                                                                          >  1:41285/1‑139 (MQ=255)                                                          ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTaa                                                                                                    <  1:752760/107‑1 (MQ=255)                                                          ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGcc                                                                    >  2:835156/1‑139 (MQ=255)                                                          ttttATCGGCTTCTTTGGGATACTAGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTaa                                                                                                    >  2:752760/1‑107 (MQ=255)                                                            ttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGcaca                                                                                                                   >  2:125716/1‑90 (MQ=255)                                                            ttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGcaca                                                                                                                   <  1:125716/90‑1 (MQ=255)                                                            ttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTaa                                                                                                    <  1:428470/105‑1 (MQ=255)                                                            ttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTaa                                                                                                    >  2:428470/1‑105 (MQ=255)                                                            ttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCg                                                                  >  1:911196/1‑139 (MQ=255)                                                                  gCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAg                                                                                                           >  2:698605/1‑92 (MQ=255)                                                                  gCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAg                                                                                                           <  1:698605/92‑1 (MQ=255)                                                                       tttGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCg                                                       <  1:1078193/139‑1 (MQ=255)                                                                             aTACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGc                                                 <  1:326669/139‑1 (MQ=255)                                                                             aTACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGc                                                 >  2:810723/1‑139 (MQ=255)                                                                                   gCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCa                                           >  2:846064/1‑139 (MQ=255)                                                                                   gCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCa                                           >  1:952721/1‑139 (MQ=255)                                                                                    ccGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAg                                          <  2:952721/139‑1 (MQ=255)                                                                                       cgATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCaa                                       <  2:704473/139‑1 (MQ=255)                                                                                         aTAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACa                                     <  1:501155/139‑1 (MQ=255)                                                                                                      ttGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                     >  2:739153/1‑46 (MQ=255)                                                                                                      ttGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                     <  1:739153/46‑1 (MQ=255)                                                                                                      ttGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAg                                          <  2:1091906/121‑1 (MQ=255)                                                                                                      ttGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAg                                          >  1:1091906/1‑121 (MQ=255)                                                                                                       tGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCtt                                                                               <  2:165247/83‑1 (MQ=255)                                                                                                       tGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCaa                                       <  2:316743/123‑1 (MQ=255)                                                                                                       tGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCaa                                       >  1:316743/1‑123 (MQ=255)                                                                                                       tGTCTGGCAAAGACATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCtt                                                                               >  1:165247/1‑83 (MQ=255)                                                                                                        gTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                      <  1:503153/43‑1 (MQ=255)                                                                                                        gTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                      >  2:503153/1‑43 (MQ=255)                                                                                                          cTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGcc                    <  1:835156/139‑1 (MQ=255)                                                                                                             gCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGt                                  <  1:376454/122‑1 (MQ=255)                                                                                                             gCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGt                                  >  2:376454/1‑122 (MQ=255)                                                                                                                       aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa       <  1:1059102/139‑1 (MQ=255)                                                                                                                       aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa       <  2:664049/139‑1 (MQ=255)                                                                                                                       aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa       <  2:41285/139‑1 (MQ=255)                                                                                                                       aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGa       <  1:810723/139‑1 (MQ=255)                                                                                                                             ttttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAg  >  2:1002304/1‑138 (MQ=255)                                                                                                                             ttttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAg  <  1:1002304/138‑1 (MQ=255)                                                                                                                              tttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTa                                                                <  2:256953/75‑1 (MQ=255)                                                                                                                              tttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTa                                                                >  1:256953/1‑75 (MQ=255)                                                                                                                                        |                                                                                                                               AGAGTTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATAAAG  >  NC_000913/821371‑821632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑