Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	826,119	C→T	*378* (TAG→TAA)	ybhS ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	826,119	0	C	T	100.0%	107.9 / NA	34	*378* (TAG→TAA)	ybhS	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/17);  total (17/17)

AACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTG  >  NC_000913/825993‑826251                                                                                                                               |                                                                                                                                     aaCGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGa                                                                                                                          >  2:931520/1‑139 (MQ=255)                     aaTTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGt                                                                                                      <  1:931520/139‑1 (MQ=255)                       ttAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCa                                                                                                    <  1:1057884/139‑1 (MQ=255)                       ttAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCa                                                                                                    <  1:495676/139‑1 (MQ=255)                         aGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAgg                                                                                                  <  2:1110377/139‑1 (MQ=255)                           cACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGcc                                                                                                >  1:567474/1‑139 (MQ=255)                               ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAg                                                                                                   >  2:159051/1‑132 (MQ=255)                               ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAg                                                                                                   <  1:159051/132‑1 (MQ=255)                               ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATa                                                                                            >  2:170934/1‑139 (MQ=255)                               ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATa                                                                                            >  1:610950/1‑139 (MQ=255)                                    aaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATCAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                       <  2:658525/139‑1 (MQ=255)                                          aaTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCAccgc                                                                                 >  2:484349/1‑139 (MQ=255)                                               cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                            <  1:170934/139‑1 (MQ=255)                                               cccgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTAGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                            >  2:667574/3‑139 (MQ=255)                                                              cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                             >  1:194614/1‑139 (MQ=255)                                                              cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCACCGCCAGGCCGATAAACATCCCCGCCGAACCGACCAAAAACAGCa                                                             >  1:700441/1‑139 (MQ=255)                                                                          aCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTAccacca                                                 >  2:46752/1‑139 (MQ=255)                                                                                aCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcacca                                           <  1:812822/139‑1 (MQ=255)                                                                                    tttGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGc                                                                                                           >  2:50964/1‑71 (MQ=255)                                                                                    tttGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGc                                                                                                           <  1:50964/71‑1 (MQ=255)                                                                                     ttGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGa                                      <  1:650567/139‑1 (MQ=255)                                                                                      tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAg                                                                                                            <  1:1095358/68‑1 (MQ=255)                                                                                      tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAg                                                                                                            >  2:1095358/1‑68 (MQ=255)                                                                                           aTTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                            <  1:553032/95‑1 (MQ=255)                                                                                           aTTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                            >  2:553032/1‑95 (MQ=255)                                                                                                cGTCCATAAGCGATGAAACATGCTCTTCGCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGc                           <  1:667574/139‑1 (MQ=255)                                                                                                         gCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAg                  <  1:484349/139‑1 (MQ=255)                                                                                                            aTGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTc                                                                                                     >  2:242126/1‑53 (MQ=255)                                                                                                            aTGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTc                                                                                                     <  1:242126/53‑1 (MQ=255)                                                                                                             tGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCtt              >  1:599379/1‑139 (MQ=255)                                                                                                             tGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCtt              >  1:408571/1‑139 (MQ=255)                                                                                                                         ttctCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTg  <  2:408571/139‑1 (MQ=255)                                                                                                                           ctcCTTTATCCAGCCGACGTTTGCTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                            <  2:734081/63‑1 (MQ=255)                                                                                                                           ctcCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                            >  1:734081/1‑63 (MQ=255)                                                                                                                               |                                                                                                                                     AACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTG  >  NC_000913/825993‑826251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑