Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 828,477 C→T V169I (GTT→ATT)  ybhF ← putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913828,4770CT100.0% 131.6 / NA 41V169I (GTT→ATT) ybhFputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/21);  total (20/21)

CGCCTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAG  >  NC_000913/828342‑828602
                                                                                                                                       |                                                                                                                             
cGCCTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAatgcc                                                                                                                            >  1:952297/1‑139 (MQ=255)
                   cacGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagc                                                                                                         <  2:941894/139‑1 (MQ=255)
                     cGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagcag                                                                                                       >  2:911782/1‑139 (MQ=255)
                                tGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCg                                                                                            >  1:579374/1‑139 (MQ=255)
                                  cTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt                                                                                          >  2:589576/1‑139 (MQ=255)
                                  cTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt                                                                                          >  2:198045/1‑139 (MQ=255)
                                  cTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt                                                                                          >  1:857818/1‑139 (MQ=255)
                                            tcgAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                <  1:198045/139‑1 (MQ=255)
                                            tcgAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                <  2:579374/139‑1 (MQ=255)
                                            tcgAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                <  1:911782/139‑1 (MQ=255)
                                            tcgAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                <  1:838274/139‑1 (MQ=255)
                                                gaTACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGt                                                                            <  2:99032/139‑1 (MQ=255)
                                                                  ggATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGttt                                                          >  1:828883/1‑139 (MQ=255)
                                                                   gATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGtttt                                                         >  1:929004/1‑139 (MQ=255)
                                                                      tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagcagc                                                                                                      <  1:359908/91‑1 (MQ=255)
                                                                      tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagcagc                                                                                                      >  2:359908/1‑91 (MQ=255)
                                                                      tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc                                                                                     <  1:836738/108‑1 (MQ=255)
                                                                      tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc                                                                                     >  2:836738/1‑108 (MQ=255)
                                                                        aCATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGttt                                                    <  2:465622/139‑1 (MQ=255)
                                                                        aCATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGttt                                                    >  2:813590/1‑139 (MQ=255)
                                                                        aCATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGttt                                                    >  1:465622/1‑139 (MQ=255)
                                                                          aTCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCTAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCa                                                  <  1:813590/139‑1 (MQ=255)
                                                                                tCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccac                                                                                  >  1:520971/1‑101 (MQ=255)
                                                                                tCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccac                                                                                  <  2:520971/101‑1 (MQ=255)
                                                                                 cgcccgccAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGt                                                                                                                  <  2:129428/68‑1 (MQ=255)
                                                                                 cgcccgccAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGt                                                                                                                  >  1:129428/1‑68 (MQ=255)
                                                                                    ccgccAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGga                                        >  1:541093/1‑139 (MQ=255)
                                                                                                    cATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAgg                        >  1:228934/1‑139 (MQ=255)
                                                                                                     aTCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt                                                                                          >  2:832980/1‑72 (MQ=255)
                                                                                                     aTCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt                                                                                          <  1:832980/72‑1 (MQ=255)
                                                                                                     aTCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                >  1:1050138/1‑82 (MQ=255)
                                                                                                     aTCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                <  2:1050138/82‑1 (MQ=255)
                                                                                                      tCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCg                      >  1:596998/1‑139 (MQ=255)
                                                                                                               gTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTaaa             <  2:268741/139‑1 (MQ=255)
                                                                                                               gTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTaaa             <  2:840861/139‑1 (MQ=255)
                                                                                                               gTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTaaa             <  2:857818/139‑1 (MQ=255)
                                                                                                                    cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGcc        <  1:412698/139‑1 (MQ=255)
                                                                                                                    cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGcc        >  2:412698/1‑139 (MQ=255)
                                                                                                                    cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGcc        <  2:929004/139‑1 (MQ=255)
                                                                                                                          gagaTAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAagag  <  1:639058/139‑1 (MQ=255)
                                                                                                                                 ggTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCg                                                                                            >  2:430882/1‑42 (MQ=255)
                                                                                                                                 ggTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCg                                                                                            <  1:430882/42‑1 (MQ=255)
                                                                                                                                       |                                                                                                                             
CGCCTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAG  >  NC_000913/828342‑828602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: