Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	866,518	T→C	intergenic (‑154/‑50)	moeA ← / → iaaA	molybdopterin molybdenumtransferase, molybdopterin biosynthesis protein/Isoaspartyl peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	866,518	0	T	C	100.0%	135.2 / NA	38	intergenic (‑154/‑50)	moeA/iaaA	molybdopterin molybdenumtransferase, molybdopterin biosynthesis protein/Isoaspartyl peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/20);  total (18/20)

AAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAAT  >  NC_000913/866385‑866643                                                                                                                                      |                                                                                                                              aaaaTCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGa                                                                                                                          >  2:209451/1‑139 (MQ=255)   aaTCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGata                                                                                                                        >  1:917696/1‑139 (MQ=255)     tCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATAtt                                                                                                                      <  2:1010178/139‑1 (MQ=255)                  ttatGTCCGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTg                                                                                                         <  2:1087275/139‑1 (MQ=255)                        cAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAac                                                                                                   >  1:916030/1‑139 (MQ=255)                         aGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAacc                                                                                                  <  2:916030/139‑1 (MQ=255)                           aaaaaCGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAaccac                                                                                                >  1:20675/1‑139 (MQ=255)                                   cacaGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATg                                                                                        <  2:20675/139‑1 (MQ=255)                                   cacaGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATg                                                                                        <  2:917696/139‑1 (MQ=255)                                        aCTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTaa                                                                                          >  2:455117/1‑132 (MQ=255)                                        aCTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTaa                                                                                          <  1:455117/132‑1 (MQ=255)                                                      cGGGCGTAGCGCTGGGTCCTGTCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAac                                                                                                   <  1:175459/109‑1 (MQ=255)                                                      cGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGaa                                                                                                    >  2:579845/1‑108 (MQ=255)                                                      cGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGaa                                                                                                    <  1:579845/108‑1 (MQ=255)                                                      cGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAac                                                                                                   >  2:175459/1‑109 (MQ=255)                                                        ggCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAac                                                                                                   >  1:276078/1‑107 (MQ=255)                                                        ggCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAac                                                                                                   <  2:276078/107‑1 (MQ=255)                                                                              ttACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAACAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGgc                                             >  1:424813/1‑139 (MQ=255)                                                                               tACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGaa                                                                                                    <  1:985751/83‑1 (MQ=255)                                                                               tACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGaa                                                                                                    >  2:985751/1‑83 (MQ=255)                                                                               tACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGgcg                                            <  1:209451/139‑1 (MQ=255)                                                                                               cTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAacc                                                                                                  >  1:54994/1‑69 (MQ=255)                                                                                               cTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAacc                                                                                                  <  2:54994/69‑1 (MQ=255)                                                                                                  tCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCAtt                                                            <  1:119193/104‑1 (MQ=255)                                                                                                  tCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCAtt                                                            >  2:119193/1‑104 (MQ=255)                                                                                                       atTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGa                    <  1:566030/139‑1 (MQ=255)                                                                                                        ttCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTg                                                           >  1:108667/1‑99 (MQ=255)                                                                                                        ttCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTg                                                           <  2:108667/99‑1 (MQ=255)                                                                                                              aaTTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTaa                                                                                          <  2:1018720/62‑1 (MQ=255)                                                                                                              aaTTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTaa                                                                                          >  1:1018720/1‑62 (MQ=255)                                                                                                                  gAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCaa         >  1:770238/1‑139 (MQ=255)                                                                                                                     tGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAg      >  1:293748/1‑139 (MQ=255)                                                                                                                        gTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGaa   <  1:15555/139‑1 (MQ=255)                                                                                                                         tAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAAt  <  2:424813/139‑1 (MQ=255)                                                                                                                          aaTTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGcc                             >  2:509356/1‑111 (MQ=255)                                                                                                                          aaTTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGcc                             <  1:509356/111‑1 (MQ=255)                                                                                                                           aTTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATg                                                                                        >  2:415610/1‑51 (MQ=255)                                                                                                                           aTTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATg                                                                                        <  1:415610/51‑1 (MQ=255)                                                                                                                                      |                                                                                                                              AAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAAT  >  NC_000913/866385‑866643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑