Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	97,297	G→A	A71T (GCC→ACC)	murD →	UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	97,297	0	G	A	100.0%	88.2 / NA	30	A71T (GCC→ACC)	murD	UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/11);  total (19/11)

CTCGCGGTGTGACGCCGCGCGTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGG  >  NC_000913/97166‑97421                                                                                                                                    |                                                                                                                             ctcgcGGTGTGACGCCGCGCGTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTcc                                                                                                                       <  1:56021/139‑1 (MQ=255)           gACGCCGCGCGTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCa                                                                                                             <  1:376547/139‑1 (MQ=255)                  cgcgTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGCACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGgcgc                                                                                                      >  2:215549/1‑139 (MQ=255)                               cgcgTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAg                                                                                         >  2:262238/1‑139 (MQ=255)                                 cgTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAgcg                                                                                       >  1:595620/1‑139 (MQ=255)                                         acCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCt                                                                               >  1:611173/1‑139 (MQ=255)                                                  cctggATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGgc                                                                                                        <  1:644831/105‑1 (MQ=255)                                                  ccaggATAAATAACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATCAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGgc                                                                                                        >  2:644831/4‑105 (MQ=255)                                                            ttACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCg                                                            <  1:262238/139‑1 (MQ=255)                                                             tACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGt                                                           >  1:70725/1‑139 (MQ=255)                                                                ccGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGa                                                                             <  1:582764/118‑1 (MQ=255)                                                                ccGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGa                                                                             >  2:582764/1‑118 (MQ=255)                                                                    aGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGat                                                    >  2:939530/1‑139 (MQ=255)                                                                     gCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGata                                                   >  2:101145/1‑139 (MQ=255)                                                                       cGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATc                                                 <  1:215549/139‑1 (MQ=255)                                                                          aGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAg                                              >  1:45275/1‑139 (MQ=255)                                                                          aGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAg                                              >  1:1102269/1‑139 (MQ=255)                                                                               gCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGtt                                         <  2:595620/139‑1 (MQ=255)                                                                                ccACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTc                                        >  1:912325/1‑139 (MQ=255)                                                                                        gCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGa                                <  2:45275/139‑1 (MQ=255)                                                                                             cTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGaagcac                           <  1:939530/139‑1 (MQ=255)                                                                                                  tgatgaATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGaagcacaagca                      >  2:281659/1‑139 (MQ=255)                                                                                                      gaATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTcg                                   >  2:9249/1‑122 (MQ=255)                                                                                                      gaATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTcg                                   <  1:9249/122‑1 (MQ=255)                                                                                                      gaATGGCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGa                  >  1:215254/1‑139 (MQ=255)                                                                                                          ggCTGATGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATtgt              >  1:930558/1‑139 (MQ=255)                                                                                                          ggCTGATGGCGCCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCAT‑CATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATtgtg             <  1:725453/139‑1 (MQ=255)                                                                                                               aTGGCGGCAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGCAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGa         >  2:551000/1‑139 (MQ=255)                                                                                                                 ggcggcAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGAtt       >  1:892935/1‑139 (MQ=255)                                                                                                                      cAGATCTGATTGTCACCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCgg  >  2:279945/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                             CTCGCGGTGTGACGCCGCGCGTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGG  >  NC_000913/97166‑97421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑