Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,006,491	C→T	V180V (GTC→GTT)  *370* (TAG→TAA)	zapC → ycbX ←	FtsZ stabilizer 6‑N‑hydroxylaminopurine detoxification oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,006,491	0	C	T	100.0%	125.6 / NA	40	V180V (GTC→GTT)  *370* (TAG→TAA)	zapC ycbX	FtsZ stabilizer 6‑N‑hydroxylaminopurine detoxification oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/20);  total (20/20)

TGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCA  >  NC_000913/1006363‑1006610                                                                                                                                 |                                                                                                                        tGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCaa                                                                                                              >  1:692503/1‑139 (MQ=255) tGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCaa                                                                                                              >  2:558840/1‑139 (MQ=255)       ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAg                                                                                                        >  2:499576/1‑139 (MQ=255)         ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                                                                                                      <  1:516072/139‑1 (MQ=255)             caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                                                                                  <  2:452462/139‑1 (MQ=255)             caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                                                                                  <  2:808047/139‑1 (MQ=255)             caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                                                                                  <  1:50005/139‑1 (MQ=255)             caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                                                                                  <  1:558840/139‑1 (MQ=255)                      gttgttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTac                                                                                         <  1:499576/139‑1 (MQ=255)                          ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                                                                                     >  2:75124/1‑139 (MQ=255)                             ttGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTa                                                                                  <  1:850856/139‑1 (MQ=255)                                  ggTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa                                                                             >  2:530525/1‑139 (MQ=255)                                       cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGaa                                                                        <  2:692503/139‑1 (MQ=255)                                             gCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGc                                                                  >  2:1103751/1‑139 (MQ=255)                                                     gCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCatcatc                                                           <  1:367161/139‑1 (MQ=255)                                                          gCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCa                                                      <  1:75124/139‑1 (MQ=255)                                                                      aTGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGcc                                                                                                                <  1:589782/69‑1 (MQ=255)                                                                      aTGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGcc                                                                                                                >  2:589782/1‑69 (MQ=255)                                                                       tGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTacac                                                                                       >  2:192238/1‑93 (MQ=255)                                                                       tGAACGACAGGCTGAAACCGCAAGTTAATGTTGACAGCTTCAGCCTCGAATAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTacac                                                                                       <  1:192238/93‑1 (MQ=255)                                                                        gAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTc                                        >  2:986127/1‑139 (MQ=255)                                                                         aaCGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCa                                       >  1:893991/1‑139 (MQ=255)                                                                              cAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAgcggc                                  >  2:152478/1‑139 (MQ=255)                                                                                 gCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                                                                                               >  1:324827/1‑75 (MQ=255)                                                                                 gCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                                                                                               <  2:324827/75‑1 (MQ=255)                                                                                  cTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGa                              >  1:682203/1‑139 (MQ=255)                                                                                      aaCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTc                          <  1:1103751/139‑1 (MQ=255)                                                                                           cAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTg                                                                   >  2:380492/1‑93 (MQ=255)                                                                                           cAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTg                                                                   <  1:380492/93‑1 (MQ=255)                                                                                                        aCAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTaca                                                                                        >  1:810226/1‑59 (MQ=255)                                                                                                        aCAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTaca                                                                                        <  2:810226/59‑1 (MQ=255)                                                                                                        aCAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAac        >  1:737429/1‑139 (MQ=255)                                                                                                         cAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAaca       >  1:355159/1‑139 (MQ=255)                                                                                                            cTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGc                        >  2:1067617/1‑119 (MQ=255)                                                                                                            cTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGc                        <  1:1067617/119‑1 (MQ=255)                                                                                                            cTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACgg    >  1:265387/1‑139 (MQ=255)                                                                                                             ttCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCATGACTTCGCCTTCTAAAAGCTGAACACGGc   <  2:893991/139‑1 (MQ=255)                                                                                                              tCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCa  <  2:355159/139‑1 (MQ=255)                                                                                                                      gAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa                                                                             <  2:355618/56‑1 (MQ=255)                                                                                                                      gAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa                                                                             >  1:355618/1‑56 (MQ=255)                                                                                                                                 |                                                                                                                        TGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCA  >  NC_000913/1006363‑1006610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑