Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,073,732 | C→T | A94T (GCG→ACG) | rutA ← | pyrimidine oxygenase, FMN‑dependent |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,073,732 | 0 | C | T | 96.9% | 93.5 / NA | 32 | A94T (GCG→ACG) | rutA | pyrimidine oxygenase, FMN‑dependent |
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (13/18); total (14/18) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATT > NC_000913/1073604‑1073867 | cACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGcc < 1:395581/139‑1 (MQ=255) aCGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCa < 2:1021479/139‑1 (MQ=255) cGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCAt < 2:563505/139‑1 (MQ=255) aaaCGCCCGCCAGAGATGGAGTCGATGGTTGCGTCCATACGGGCGACGATTGCCGGAGGTGACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGGCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACt > 2:1112033/1‑139 (MQ=255) aaaCGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACt < 1:410353/139‑1 (MQ=255) tGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCa < 1:404205/139‑1 (MQ=255) gAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGa > 2:834745/1‑139 (MQ=255) tGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGt > 1:558872/1‑139 (MQ=255) cGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCGGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAg > 2:12504/1‑85 (MQ=255) cGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAg < 1:12504/85‑1 (MQ=255) tACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCa < 1:1050849/104‑1 (MQ=255) tACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCa > 2:1050849/1‑104 (MQ=255) tACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCaaa < 2:558872/139‑1 (MQ=255) ggCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCa < 1:834745/139‑1 (MQ=255) ggCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCa < 1:343435/139‑1 (MQ=255) aTTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGtt < 2:587595/139‑1 (MQ=255) aTTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTAAATGCACGACGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCCGTTTTGCCGCCAAAGCCACGCAGtt < 1:1112033/139‑1 (MQ=255) tGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTtgt > 1:492092/1‑98 (MQ=255) tGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTtgt < 2:492092/98‑1 (MQ=255) gTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcgaaat < 1:207967/139‑1 (MQ=255) tGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGGGAATGACTAAAGGTTGTGATCCCAGAACTCTGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAAgggaa > 2:479105/1‑137 (MQ=255) cGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTg > 2:272698/1‑139 (MQ=255) aTCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcgaaat < 1:819989/121‑1 (MQ=255) aTCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcgaaat > 2:819989/1‑121 (MQ=255) cTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAg > 1:585907/1‑40 (MQ=255) cTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAg < 2:585907/40‑1 (MQ=255) gAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTg > 2:890294/1‑41 (MQ=255) gAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTg < 1:890294/41‑1 (MQ=255) gAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa < 2:391528/139‑1 (MQ=255) aCGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCtt > 2:385192/1‑139 (MQ=255) cGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCttt > 2:612734/1‑139 (MQ=255) cGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCttt > 1:1054653/1‑139 (MQ=255) ccgtcAGCCCCGCCATCAAGGCGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTAtt < 2:770741/139‑1 (MQ=255) | CACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATT > NC_000913/1073604‑1073867 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |