Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,073,732 C→T A94T (GCG→ACG)  rutA ← pyrimidine oxygenase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,073,7320CT96.9% 93.5 / NA 32A94T (GCG→ACG) rutApyrimidine oxygenase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (13/18);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATT  >  NC_000913/1073604‑1073867
                                                                                                                                |                                                                                                                                       
cACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGcc                                                                                                                               <  1:395581/139‑1 (MQ=255)
 aCGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCa                                                                                                                              <  2:1021479/139‑1 (MQ=255)
  cGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCAt                                                                                                                             <  2:563505/139‑1 (MQ=255)
                aaaCGCCCGCCAGAGATGGAGTCGATGGTTGCGTCCATACGGGCGACGATTGCCGGAGGTGACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGGCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACt                                                                                                               >  2:1112033/1‑139 (MQ=255)
                aaaCGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACt                                                                                                               <  1:410353/139‑1 (MQ=255)
                                tGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCa                                                                                               <  1:404205/139‑1 (MQ=255)
                                  gAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGa                                                                                             >  2:834745/1‑139 (MQ=255)
                                         tGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGt                                                                                      >  1:558872/1‑139 (MQ=255)
                                               cGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCGGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAg                                                                                                                                      >  2:12504/1‑85 (MQ=255)
                                               cGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAg                                                                                                                                      <  1:12504/85‑1 (MQ=255)
                                                     tACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCa                                                                                                             <  1:1050849/104‑1 (MQ=255)
                                                     tACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCa                                                                                                             >  2:1050849/1‑104 (MQ=255)
                                                     tACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCaaa                                                                          <  2:558872/139‑1 (MQ=255)
                                                         ggCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCa                                                                      <  1:834745/139‑1 (MQ=255)
                                                         ggCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCa                                                                      <  1:343435/139‑1 (MQ=255)
                                                                aTTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGtt                                                               <  2:587595/139‑1 (MQ=255)
                                                                aTTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTAAATGCACGACGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCCGTTTTGCCGCCAAAGCCACGCAGtt                                                               <  1:1112033/139‑1 (MQ=255)
                                                                  tGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTtgt                                                                                                      >  1:492092/1‑98 (MQ=255)
                                                                  tGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTtgt                                                                                                      <  2:492092/98‑1 (MQ=255)
                                                                                     gTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcgaaat                                          <  1:207967/139‑1 (MQ=255)
                                                                                               tGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGGGAATGACTAAAGGTTGTGATCCCAGAACTCTGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAAgggaa                                >  2:479105/1‑137 (MQ=255)
                                                                                                  cGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTg                             >  2:272698/1‑139 (MQ=255)
                                                                                                       aTCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcgaaat                                          <  1:819989/121‑1 (MQ=255)
                                                                                                       aTCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcgaaat                                          >  2:819989/1‑121 (MQ=255)
                                                                                                         cTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAg                                                                                                                         >  1:585907/1‑40 (MQ=255)
                                                                                                         cTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAg                                                                                                                         <  2:585907/40‑1 (MQ=255)
                                                                                                           gAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTg                                                                                                                      >  2:890294/1‑41 (MQ=255)
                                                                                                           gAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTg                                                                                                                      <  1:890294/41‑1 (MQ=255)
                                                                                                                   gAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa            <  2:391528/139‑1 (MQ=255)
                                                                                                                         aCGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCtt      >  2:385192/1‑139 (MQ=255)
                                                                                                                          cGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCttt     >  2:612734/1‑139 (MQ=255)
                                                                                                                          cGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCttt     >  1:1054653/1‑139 (MQ=255)
                                                                                                                             ccgtcAGCCCCGCCATCAAGGCGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTAtt  <  2:770741/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                       
CACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATT  >  NC_000913/1073604‑1073867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: