Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,100,304	A→G	I3M (ATA→ATG)	ycdZ →	DUF1097 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,100,304	0	A	G	100.0%	218.8 / NA	60	I3M (ATA→ATG)	ycdZ	DUF1097 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/32);  total (28/32)

AGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTGGGCGGGCTTCCTGGGCTGTACGGCCTACTTTGCCTGCCCGCAAGGTGGGCTGAA  >  NC_000913/1100169‑1100438                                                                                                                                        |                                                                                                                                       aGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCtt                                                                                                                                     <  1:1031870/139‑1 (MQ=255)   cTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTtct                                                                                                                                   <  2:111517/139‑1 (MQ=255)    tGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCtt                                                                                                                                     <  1:332429/136‑1 (MQ=255)    tGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCtt                                                                                                                                     >  2:332429/1‑136 (MQ=255)             aTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCa                                                                                                                         >  2:805402/1‑139 (MQ=255)               tCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAAt                                                                                                                       <  1:836162/139‑1 (MQ=255)                 tGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATca                                                                                                                     >  2:40824/1‑139 (MQ=255)                          aGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCa                                                                                                            <  1:494758/139‑1 (MQ=255)                            tgtgATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCAtt                                                                                                          <  2:265937/139‑1 (MQ=255)                            tgtgATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCAtt                                                                                                          <  1:919642/139‑1 (MQ=255)                                aTCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATtctct                                                                                                      >  1:924458/1‑139 (MQ=255)                                aTCTACGTCACTCATAAATGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGTTCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATtctct                                                                                                      >  2:431422/1‑139 (MQ=255)                                     cGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCacaa                                                                                                                  >  2:514560/1‑122 (MQ=255)                                     cGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCacaa                                                                                                                  <  1:514560/122‑1 (MQ=255)                                      gTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTa                                                                                                >  1:434320/1‑139 (MQ=255)                                          cTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTtct                                                                                                                                   <  2:589888/100‑1 (MQ=255)                                          cTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTtct                                                                                                                                   >  1:589888/1‑100 (MQ=255)                                          cTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAg                                                                                                               >  1:260713/1‑120 (MQ=255)                                          cTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAg                                                                                                               <  2:260713/120‑1 (MQ=255)                                          cTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCg                                                                                                   >  2:57896/1‑132 (MQ=255)                                          cTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCg                                                                                                   <  1:57896/132‑1 (MQ=255)                                          cTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTATCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTg                                                                                            >  1:693535/1‑139 (MQ=255)                                              tAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGa                                                                                        <  2:334572/139‑1 (MQ=255)                                                    cAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCg                                                                                                   >  1:969559/1‑122 (MQ=255)                                                    cAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCg                                                                                                   <  2:208898/122‑1 (MQ=255)                                                    cAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCg                                                                                                   <  2:969559/122‑1 (MQ=255)                                                    cAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCg                                                                                                   >  1:208898/1‑122 (MQ=255)                                                            aaTTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTtct                                                                                                                                   >  1:278918/1‑82 (MQ=255)                                                            aaTTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTtct                                                                                                                                   <  2:278918/82‑1 (MQ=255)                                                            aaTTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCa                                                                                                                         >  1:480155/1‑92 (MQ=255)                                                            aaTTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCa                                                                                                                         <  2:480155/92‑1 (MQ=255)                                                              tttGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTccc                                                                        <  1:216449/139‑1 (MQ=255)                                                              tttGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTccc                                                                        <  1:40824/139‑1 (MQ=255)                                                                 gttgttgCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTgg                                                                     <  1:479718/139‑1 (MQ=255)                                                                 gttgttgCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTgg                                                                     <  1:805402/139‑1 (MQ=255)                                                                   tgttgCATAAAATGTGCGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGc                                                                   >  2:676620/1‑139 (MQ=255)                                                                       gCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTAc                                                               >  1:990473/1‑139 (MQ=255)                                                                          tAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCt                                                                                                                                      >  1:537450/1‑65 (MQ=255)                                                                          tAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCt                                                                                                                                      <  2:537450/65‑1 (MQ=255)                                                                                  gtgCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTgggcg                                                    >  1:300317/1‑139 (MQ=255)                                                                                   tgCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTgggcgg                                                   >  2:457169/1‑139 (MQ=255)                                                                                                 catGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTGGGCGTGCTTCCTGGGCTGt                                     <  1:431422/139‑1 (MQ=255)                                                                                                 catGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTGGGCGGGCTTCCTGGGCTGt                                     <  1:128438/139‑1 (MQ=255)                                                                                                    ggCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATcac                                                                                                                    <  1:1025441/57‑1 (MQ=255)                                                                                                    ggCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATcac                                                                                                                    >  2:1025441/1‑57 (MQ=255)                                                                                                    ggCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCg                                                                                                   >  1:1082432/1‑74 (MQ=255)                                                                                                    ggCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCg                                                                                                   <  2:1082432/74‑1 (MQ=255)                                                                                                    ggCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTAt                                                                                               >  2:232621/1‑78 (MQ=255)                                                                                                    ggCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTAt                                                                                               <  1:232621/78‑1 (MQ=255)                                                                                                    ggCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGt                                                                             >  2:197909/1‑96 (MQ=255)                                                                                                    ggCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGt                                                                             <  1:197909/96‑1 (MQ=255)                                                                                                    ggCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTc                                                                          >  2:498365/1‑99 (MQ=255)                                                                                                    ggCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTc                                                                          <  1:498365/99‑1 (MQ=255)                                                                                                    ggCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTGGGCGGGCTTCCTGGGCTGTACg                                  >  1:368524/1‑139 (MQ=255)                                                                                                     gCCGCGTTTTCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTGGGCGGGCTTCCTGGGCTGTACgg                                 <  1:177784/139‑1 (MQ=255)                                                                                                              tCTGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTGGGCGGGCTTCCTGGGCTGTACGGCCTACTTtg                        >  2:605855/1‑139 (MQ=255)                                                                                                                tGCTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTGGGCGGGCTTCCTGGGCTGTACGGCCTACTTtgcc                      <  2:990473/139‑1 (MQ=255)                                                                                                                  cTATCATGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTGGGCGGGCTTCCTGGGCTGTACGGCCTACTTtgcctc                    <  2:693535/139‑2 (MQ=255)                                                                                                                        tGCGCGGCATGAACATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTGGGCGGGCTTCCTGGGCTGTACGGCCTACTTTGCCTGCCCGCa              >  2:120348/1‑139 (MQ=255)                                                                                                                                    aCATGCTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTGGGCGGGCTTCCTGGGCTGTACGGCCTACTTTGCCTGCCCGCAAGGTGGGCTGaa  <  2:300317/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       AGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTTACTAAGCTGGGCGGGCTTCCTGGGCTGTACGGCCTACTTTGCCTGCCCGCAAGGTGGGCTGAA  >  NC_000913/1100169‑1100438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑