Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,647	A→G	V237A (GTC→GCC)	yaaW ←	UPF0174 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,647	0	A	G	100.0%	119.4 / NA	34	V237A (GTC→GCC)	yaaW	UPF0174 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/15);  total (19/15)

CGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATT  >  NC_000913/10515‑10744                                                                                                                                     |                                                                                                  cGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAg                                                                                             <  2:547100/139‑1 (MQ=255)   aatgaatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTg                                                                                           >  1:923110/1‑139 (MQ=255)                    tttCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGgcg                                                                          <  2:355592/139‑1 (MQ=255)                    tttCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGgcg                                                                          <  1:99765/139‑1 (MQ=255)                      tCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCgca                                                                        >  2:578974/1‑139 (MQ=255)                                  tGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGAttt                                                            >  1:333502/1‑139 (MQ=255)                                   gTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                           <  1:842822/139‑1 (MQ=255)                                         ttttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAg                                                                                             <  1:410037/99‑1 (MQ=255)                                         ttttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAg                                                                                             >  2:410037/1‑99 (MQ=255)                                         ttttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAg                                                        >  2:442381/1‑136 (MQ=255)                                         ttttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAg                                                        <  1:442381/136‑1 (MQ=255)                                           ttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCgcagg                                                                      >  2:860701/1‑120 (MQ=255)                                           ttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCgcagg                                                                      <  1:860701/120‑1 (MQ=255)                                           ttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACgg                                                   >  1:962538/1‑139 (MQ=255)                                              aGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAg                                                                                             >  1:732137/1‑94 (MQ=255)                                              aGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAg                                                                                             <  2:732137/94‑1 (MQ=255)                                                   gcgGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATc                                           >  2:465807/1‑139 (MQ=255)                                                                      gTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGt                                                       <  1:739605/108‑1 (MQ=255)                                                                      gTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGt                                                       >  2:739605/1‑108 (MQ=255)                                                                      gTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGc                        >  1:650203/1‑139 (MQ=255)                                                                       tGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGt                                                                                            <  1:569998/70‑1 (MQ=255)                                                                       tGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGt                                                                                            >  2:569998/1‑70 (MQ=255)                                                                       tGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGcc                       >  2:48996/1‑139 (MQ=255)                                                                                 tCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCg                                                                >  1:510707/1‑88 (MQ=255)                                                                                 tCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCg                                                                <  2:510707/88‑1 (MQ=255)                                                                                 tCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAc                 <  1:143908/135‑1 (MQ=255)                                                                                 tCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAc                 >  2:143908/1‑135 (MQ=255)                                                                                      gATCTTTTGAGGGGAAAATGAGAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAAt                                            <  1:239818/103‑1 (MQ=255)                                                                                      gATCTTTTGAGGGGAAAATGAGAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAAt                                            >  2:239818/1‑103 (MQ=255)                                                                                           tttGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGaa                                             <  1:320963/97‑1 (MQ=255)                                                                                           tttGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGaa                                             >  2:320963/1‑97 (MQ=255)                                                                                           tttGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCAt   >  1:474388/1‑139 (MQ=255)                                                                                                      aatgaaaatTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCAtt  <  1:155304/129‑1 (MQ=255)                                                                                                      aatgaaaatTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCAtt  >  2:155304/1‑129 (MQ=255)                                                                                                                                     |                                                                                                  CGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATT  >  NC_000913/10515‑10744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑