Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,211,652 | C→T | intergenic (+75/+28) | icdC → / ← iraM | pseudogene, isocitrate dehydrogenase C‑terminal gene fragment, idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,211,652 | 0 | C | T | 100.0% | 80.6 / NA | 26 | intergenic (+75/+28) | icdC/iraM | pseudogene, isocitrate dehydrogenase C‑terminal gene fragment, idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor |
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/16); total (10/16) |
AGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTT > NC_000913/1211520‑1211777 | aGGCGCTAAGCTGCTGCAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCg < 1:791805/139‑1 (MQ=255) gggcTAAGCTGCTGAAATGTTCAGAGGTTGGTGAAGCGATCATCGAAAACATGTAATATCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCTCGTTGTTTTATTTATGTTGATATTATTAATAGTATATCGAg < 2:658710/137‑1 (MQ=38) cgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAg < 1:28592/138‑1 (MQ=255) cgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAg > 2:28592/1‑138 (MQ=255) cgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGc < 2:640258/139‑1 (MQ=255) cTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAg < 1:1055931/136‑1 (MQ=255) cTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAg > 2:1055931/1‑136 (MQ=255) cTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCat > 1:46261/1‑139 (MQ=255) gAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGa < 2:253631/139‑1 (MQ=255) cGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATg > 2:335971/1‑139 (MQ=255) atcatcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAg > 1:848078/1‑116 (MQ=255) atcatcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAg < 2:848078/116‑1 (MQ=255) tGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATTTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATaa < 2:794925/139‑1 (MQ=255) tAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATg < 1:335971/139‑1 (MQ=255) ctcCATGTGTTAAATATTGAAACGGGCGTATAACACGCTCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGTCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACta < 1:472997/139‑1 (MQ=255) aTGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCa > 2:716503/1‑95 (MQ=255) aTGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCa < 1:716503/95‑1 (MQ=255) tGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACatttatt > 1:896442/1‑139 (MQ=255) gggCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCa > 1:1062205/1‑59 (MQ=255) gggCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCa < 2:1062205/59‑1 (MQ=255) cGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATg < 2:291034/66‑1 (MQ=255) cGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGTATATCGAGCATATTTATATg > 1:291034/1‑66 (MQ=255) atatTATTAATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCttt < 2:527703/139‑1 (MQ=255) attaATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTtat > 1:959483/1‑66 (MQ=255) attaATAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTtat < 2:959483/66‑1 (MQ=255) tAGTATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATaa < 2:1049812/63‑1 (MQ=255) | AGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTT > NC_000913/1211520‑1211777 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |