Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,354,505 C→T S5N (AGC→AAC) 
*322* (TAG→TAA
sapC ←
sapB ←
antimicrobial peptide transport ABC transporter permease
antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,354,5050CT100.0% 120.0 / NA 38S5N (AGC→AAC) 
*322* (TAG→TAA
sapC
sapB
antimicrobial peptide transport ABC transporter permease
antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/22);  total (16/22)

CGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTG  >  NC_000913/1354369‑1354636
                                                                                                                                        |                                                                                                                                   
cgcAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTa                                                                                                                                   >  1:537013/1‑139 (MQ=255)
         gccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAg                                                                                                                            <  1:1040727/137‑1 (MQ=255)
         gccgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAg                                                                                                                            >  2:1040727/1‑137 (MQ=255)
               aaaaaTACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCa                                                                                                                    >  2:1053700/1‑139 (MQ=255)
                 aaaTACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCAtt                                                                                                                  <  2:695792/139‑1 (MQ=255)
                    tACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCt                                                                                                               <  2:535594/139‑1 (MQ=255)
                           tACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGttt                                                                                                        >  1:74723/1‑139 (MQ=255)
                            aCAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTc                                                                                                       <  2:235934/139‑1 (MQ=255)
                             cagccagcCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                                      <  2:419447/139‑1 (MQ=255)
                             cagccagcCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                                      <  2:74723/139‑1 (MQ=255)
                                ccagccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCagag                                                                                                   >  1:324732/1‑139 (MQ=255)
                                    ccccGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGtt                                                                                               <  1:1053700/139‑1 (MQ=255)
                                     cccGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTa                                                                                              >  2:13857/1‑139 (MQ=255)
                                       cgcgCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGc                                                                                            >  1:100280/1‑139 (MQ=255)
                                              ccGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCa                                                                                     <  1:1034834/139‑1 (MQ=255)
                                                        cGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCg                                                                                                                                >  1:762223/1‑86 (MQ=255)
                                                        cGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCg                                                                                                                                <  2:762223/86‑1 (MQ=255)
                                                           ccATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAg                                                                        >  2:1103512/1‑139 (MQ=255)
                                                               cGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAAt                                                                    <  1:628269/139‑1 (MQ=255)
                                                                         tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATg                                                                                                           >  2:1086304/1‑90 (MQ=255)
                                                                         tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATg                                                                                                           <  1:1086304/90‑1 (MQ=255)
                                                                                     ttGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGt                                              <  1:376042/139‑1 (MQ=255)
                                                                                       gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATg                                                                                        >  1:1065830/1‑95 (MQ=255)
                                                                                       gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATg                                                                                        <  2:1065830/95‑1 (MQ=255)
                                                                                       gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGa                                            >  1:323992/1‑139 (MQ=255)
                                                                                       gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGa                                            <  1:13857/139‑1 (MQ=255)
                                                                                             ggCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGATCcaca                                      <  2:324732/139‑1 (MQ=255)
                                                                                             ggCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCcaca                                      <  1:314421/139‑1 (MQ=255)
                                                                                                         cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTAt                                                                                                            >  1:588558/1‑57 (MQ=255)
                                                                                                         cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTAt                                                                                                            <  2:588558/57‑1 (MQ=255)
                                                                                                         cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCaga                                                                                                    >  2:315401/1‑65 (MQ=255)
                                                                                                         cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCaga                                                                                                    <  1:315401/65‑1 (MQ=255)
                                                                                                          gTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCcg                         <  1:1103512/139‑1 (MQ=255)
                                                                                                                 gcgggcgCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCtt                                                                                                              <  2:915070/47‑1 (MQ=255)
                                                                                                                 gcgggcgCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCtt                                                                                                              >  1:915070/1‑47 (MQ=255)
                                                                                                                 gcgggcgCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                                      <  2:592201/55‑1 (MQ=255)
                                                                                                                 gcgggcgCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                                      >  1:592201/1‑55 (MQ=255)
                                                                                                                                 gTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCctg  <  2:100280/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
CGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTG  >  NC_000913/1354369‑1354636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: