Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,418,386	G→A	intergenic (‑157/‑285)	kilR ← / → sieB	killing protein, Rac prophage, FtsZ inhibitor protein/phage superinfection exclusion protein, Rac prophage

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,418,386	0	G	A	100.0%	65.9 / NA	22	intergenic (‑157/‑285)	kilR/sieB	killing protein, Rac prophage, FtsZ inhibitor protein/phage superinfection exclusion protein, Rac prophage
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/10);  total (12/10)

TGTTGATGCGGTGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTT  >  NC_000913/1418258‑1418507                                                                                                                                 |                                                                                                                          tgttgATGCGGTGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACaaaa                                                                                                                 >  1:778222/1‑139 (MQ=255)      aTGCGGTGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCAc                                                                                                            >  1:446501/1‑139 (MQ=255)                     ccTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGa                                                                                             >  2:754398/1‑139 (MQ=255)                        ccAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACAt                                                                                          >  2:729272/1‑139 (MQ=255)                        ccAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACAt                                                                                          >  1:281931/1‑139 (MQ=255)                                 tGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAAc                                                                                 >  2:805412/1‑139 (MQ=255)                                                   aCAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGCACTTCAAGAAAAAAACTGAt                                                               <  1:754398/139‑1 (MQ=255)                                                         ggCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGAc                                                                                            <  1:327510/104‑1 (MQ=255)                                                         ggCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGAc                                                                                            >  2:327510/1‑104 (MQ=255)                                                             ttCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGa                                                     <  2:281931/139‑1 (MQ=255)                                                                        aCCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGc                                          <  1:729272/139‑1 (MQ=255)                                                                                  aCTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATgccgc                                                          <  1:1023635/113‑1 (MQ=255)                                                                                  aCTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATgccgc                                                          >  2:1023635/1‑113 (MQ=255)                                                                                  aCTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTATACACAGCAATGTCGTTa                                >  1:685441/1‑139 (MQ=255)                                                                                  aCTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTa                                >  1:23391/1‑139 (MQ=255)                                                                                                tAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAgg                  <  2:684689/139‑1 (MQ=255)                                                                                                tAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAgg                  <  2:68575/139‑1 (MQ=255)                                                                                                          aCGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCcca        <  1:805412/139‑1 (MQ=255)                                                                                                           cGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTaca                                                >  2:748633/1‑98 (MQ=255)                                                                                                           cGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTaca                                                <  1:748633/98‑1 (MQ=255)                                                                                                              gcgcTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCat    <  2:446501/139‑1 (MQ=255)                                                                                                                gcTTAGCCGCATTCACCACATCACAAAATTCACTTTCAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCCGGACTACACACAGCAATGTCGTTATTTTCAACCGGTGGCGCACTCCCACCattt  >  1:1106904/1‑139 (MQ=38)                                                                                                                                 |                                                                                                                          TGTTGATGCGGTGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTT  >  NC_000913/1418258‑1418507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑