Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 130,233 T→C N343S (AAC→AGC)  yacH ← DUF3300 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913130,2330TC100.0% 304.5 / NA 83N343S (AAC→AGC) yacHDUF3300 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/35);  total (48/35)

CCGTTGACATCGGTTTGATGAAACCGCTTTGCCATATCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTG  >  NC_000913/130098‑130367
                                                                                                                                       |                                                                                                                                      
ccgTTGACATCGGTTTGATGAAACCGCTTTGCCATATCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATCGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGa                                                                                                                                     <  1:747320/139‑1 (MQ=255)
                gatgaAACCGCTTTGCCATATCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGAtgtt                                                                                                                     >  2:449373/1‑139 (MQ=255)
                 atgaAACCGCTTTGCCATATCCTGATCATGATAGGGCACACAATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATACTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGAtgttg                                                                                                                    >  2:685724/1‑139 (MQ=255)
                                         aTCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGAtgttg                                                                                                                    >  2:1090685/1‑115 (MQ=255)
                                         aTCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGAtgttg                                                                                                                    <  1:1090685/115‑1 (MQ=255)
                                         aTCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTa                                                                                            >  2:632136/1‑139 (MQ=255)
                                         aTCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTa                                                                                            >  1:868973/1‑139 (MQ=255)
                                         aTCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATCGATGTTGTCGCCGTTGTGTTGCCAGCCATTa                                                                                            >  2:584632/1‑139 (MQ=255)
                                              gATAGGGCACACCATTACGGTAGTTTGGATTGTGCCTCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTc                                                                                       >  1:112894/1‑139 (MQ=255)
                                              gATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTc                                                                                       >  2:596526/1‑139 (MQ=255)
                                              gATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTc                                                                                       >  1:449852/1‑139 (MQ=255)
                                              gATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTc                                                                                       >  1:302178/1‑139 (MQ=255)
                                               aTAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCa                                                                                      <  2:302178/139‑1 (MQ=255)
                                                tAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCAc                                                                                     >  2:80661/1‑139 (MQ=255)
                                                   ggCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGAt                                                                                  >  1:370038/1‑139 (MQ=255)
                                                   ggCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGAt                                                                                  >  1:105891/1‑139 (MQ=255)
                                                     cacaCCATTACGGTAGTTTGGGTTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACg                                                                                    >  1:212093/1‑135 (MQ=255)
                                                     cacaCCATTACGGTAGTTTGGGTTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACg                                                                                    <  2:212093/135‑1 (MQ=255)
                                                      acacCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGAc                                                                               <  2:868973/139‑1 (MQ=255)
                                                        acCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGAt                                                                                  >  1:1094986/1‑134 (MQ=255)
                                                        acCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGAt                                                                                  <  2:1094986/134‑1 (MQ=255)
                                                        acCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCg                                                                             >  2:332303/1‑139 (MQ=255)
                                                        acCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCg                                                                             >  2:129212/1‑139 (MQ=255)
                                                           aTTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCa                                                                          <  1:332303/139‑1 (MQ=255)
                                                             tACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTgc                                                                                                     >  1:87561/1‑110 (MQ=255)
                                                             tACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTgc                                                                                                     <  2:87561/110‑1 (MQ=255)
                                                                  tAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgtggt                                                                   <  1:129212/139‑1 (MQ=255)
                                                                    gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtga                                                                 <  1:596526/139‑1 (MQ=255)
                                                                    gTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtga                                                                 >  2:132100/1‑139 (MQ=255)
                                                                     tttGGATTGTGCCTCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtgat                                                                <  2:112894/139‑1 (MQ=255)
                                                                      ttGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtgatg                                                               >  2:982861/1‑139 (MQ=255)
                                                                        ggATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTgatgat                                                             <  1:132100/139‑1 (MQ=255)
                                                                              tgCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGat                                                                                                                              <  2:1003899/68‑1 (MQ=255)
                                                                              tgCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGat                                                                                                                              >  1:1003899/1‑68 (MQ=255)
                                                                              tgCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAatcat                                                       >  2:482993/1‑139 (MQ=255)
                                                                              tgCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAatcat                                                       >  1:303703/1‑139 (MQ=255)
                                                                              tgCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAatcat                                                       >  2:913385/1‑139 (MQ=255)
                                                                              tgCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATAATAatcat                                                       <  1:40181/139‑1 (MQ=255)
                                                                                  gccatgccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCAtt                                                   <  1:449373/139‑1 (MQ=255)
                                                                                   ccatgccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCg                                                                                                                 >  2:989116/1‑76 (MQ=255)
                                                                                   ccatgccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCg                                                                                                                 <  1:989116/76‑1 (MQ=255)
                                                                                        ccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatg                                             <  1:632136/139‑1 (MQ=255)
                                                                                              tCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatg                                       <  1:913385/139‑1 (MQ=255)
                                                                                              tCTTATCAGTAAGATACTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatg                                       <  1:685724/139‑1 (MQ=255)
                                                                                                 tATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTc                                                <  1:782602/127‑1 (MQ=255)
                                                                                                 tATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTc                                                >  2:782602/1‑127 (MQ=255)
                                                                                                  aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTgcc                                                                                                    >  2:1107771/1‑74 (MQ=255)
                                                                                                  aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTgcc                                                                                                    <  1:1107771/74‑1 (MQ=255)
                                                                                                  aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatg                                       >  2:632422/1‑135 (MQ=255)
                                                                                                  aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatg                                       <  1:632422/135‑1 (MQ=255)
                                                                                                  aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATgg                                   >  2:17066/1‑139 (MQ=255)
                                                                                                  aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATgg                                   >  2:197310/1‑139 (MQ=255)
                                                                                                  aTCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATgg                                   >  2:200306/1‑139 (MQ=255)
                                                                                                     aggaaGATGCTAACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGCTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCa                                >  2:644174/4‑139 (MQ=255)
                                                                                                       tAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACg                                                                                    >  2:175826/1‑85 (MQ=255)
                                                                                                       tAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACg                                                                                    <  1:175826/85‑1 (MQ=255)
                                                                                                       tAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATg                              >  2:648234/1‑139 (MQ=255)
                                                                                                       tAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATg                              >  1:1058485/1‑139 (MQ=255)
                                                                                                       tAAGATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATg                              >  2:278928/1‑139 (MQ=255)
                                                                                                          gATGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtc                           <  2:303703/139‑1 (MQ=255)
                                                                                                            tGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgt                         >  2:160049/1‑139 (MQ=255)
                                                                                                            tGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtagt                         >  2:409563/1‑139 (MQ=255)
                                                                                                            tGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCAATCGTGGTGATGATAATCATCATTGTCATGATGATGAAGGTCATGTtcgt                         >  2:870859/1‑139 (MQ=255)
                                                                                                            tGCTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGAAGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgt                         >  1:827728/1‑139 (MQ=255)
                                                                                                              cTCACCGGTGTTACGGTTGAAATTGCAGACGTAGATATTGGTGTTGTCGCCGTTCTGTTGCCAGCCATTACCGCCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTAATGATGATGATGGTCATGAtcgtcg                       <  1:870859/139‑1 (MQ=255)
                                                                                                              cTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGcc                                                                                                               <  2:1085366/51‑1 (MQ=255)
                                                                                                              cTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGcc                                                                                                               >  1:1085366/1‑51 (MQ=255)
                                                                                                              cTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtga                                                                 >  1:600538/1‑97 (MQ=255)
                                                                                                              cTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtga                                                                 <  2:600538/97‑1 (MQ=255)
                                                                                                              cTCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcg                       <  1:644174/139‑1 (MQ=255)
                                                                                                               tCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatg                                       >  2:485422/1‑122 (MQ=255)
                                                                                                               tCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCatgatgatg                                       <  1:485422/122‑1 (MQ=255)
                                                                                                               tCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgt                      <  1:160049/139‑1 (MQ=255)
                                                                                                               tCACCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgt                      >  1:528508/1‑139 (MQ=255)
                                                                                                                 aCCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgtcg                    >  2:416843/1‑139 (MQ=255)
                                                                                                                 aCCGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGAGAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgtcg                    >  2:666446/1‑139 (MQ=255)
                                                                                                                   cGGTGATACGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCTTTGTCATGATGATGATGGTCATGATCgtcgtcgtc                  <  2:827728/139‑1 (MQ=255)
                                                                                                                           cGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGa          <  2:105891/139‑1 (MQ=255)
                                                                                                                           cGGTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGa          <  1:892678/139‑1 (MQ=255)
                                                                                                                             gTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCAt                                                                                              <  2:1000165/53‑1 (MQ=255)
                                                                                                                             gTTGAAATTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCAt                                                                                              >  1:1000165/1‑53 (MQ=255)
                                                                                                                                  aaTTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATgctgct   <  1:80661/139‑1 (MQ=255)
                                                                                                                                   aTTGCTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGctgctg  >  2:275608/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CCGTTGACATCGGTTTGATGAAACCGCTTTGCCATATCCTGATCATGATAGGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTG  >  NC_000913/130098‑130367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: