| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,557,112 | C→T | *309* (TAG→TAA) | ddpF ← | D,D‑dipeptide ABC transporter ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,557,112 | 0 | C | T | 96.0% | 74.2 / ‑6.8 | 25 | *309* (TAG→TAA) | ddpF | D,D‑dipeptide ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (14/10); minor base G (0/1); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGT‑GGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGT > NC_000913/1556987‑1557239 | aaaagcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGcgcccgcc > 2:445972/1‑139 (MQ=255) ggATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg < 1:298234/139‑1 (MQ=255) aTGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACACTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGa > 2:937277/1‑139 (MQ=255) ctctcAGGTACTGAAAGCGGAAATTATGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtc > 2:487346/1‑139 (MQ=255) aCTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTccc > 2:543755/1‑139 (MQ=255) aCTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTccc > 1:156477/1‑139 (MQ=255) tGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCtt < 1:84112/139‑1 (MQ=255) cTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg < 2:133061/139‑1 (MQ=255) aaaGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg > 1:19806/1‑65 (MQ=255) aaaGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg < 2:19806/65‑1 (MQ=255) aaaGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg < 2:345295/117‑1 (MQ=255) aaaGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg > 1:345295/1‑117 (MQ=255) aaaGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGT‑GGGTTGcc < 1:487346/139‑1 (MQ=255) tGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGT‑GGGTTGCCAGCGGGc < 2:156477/139‑1 (MQ=255) gCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTGTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGT‑GGGTTGCCAGCGGGCa < 2:750406/139‑1 (MQ=255) tGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGT‑GGGTTGCCAGCGGGCAACGTTc > 2:1013008/1‑139 (MQ=255) aCGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGTGCCCGCCAGCACCg > 1:79471/1‑62 (MQ=255) aCGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGTGCCCGCCAGCACCg < 2:79471/62‑1 (MQ=255) aGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGGTTGCCAGCGGGCAACGTTCGTAGaaaa > 2:823324/1‑139 (MQ=255) tGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgt < 2:639352/54‑1 (MQ=255) tGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgt > 1:639352/1‑54 (MQ=255) tGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGT‑GGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGt > 1:732624/1‑139 (MQ=255) tgttAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGT‑GGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGt > 1:550013/1‑139 (MQ=255) gCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCt < 2:589579/48‑1 (MQ=255) gCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCt > 1:589579/1‑48 (MQ=255) | AAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGT‑GGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGT > NC_000913/1556987‑1557239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |