Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,605,359	A→G	K103K (AAA→AAG)	lsrB →	autoinducer 2‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,605,359	0	A	G	100.0%	227.1 / NA	63	K103K (AAA→AAG)	lsrB	autoinducer 2‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/33);  total (30/33)

GGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGG  >  NC_000913/1605241‑1605492                                                                                                                       |                                                                                                                                        ggCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACg                                                                                                                           <  1:125883/133‑1 (MQ=255) ggCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACg                                                                                                                           >  2:125883/1‑133 (MQ=255) ggCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGgtgt                                                                                                                     >  1:342134/1‑139 (MQ=255) ggCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGgtgt                                                                                                                     >  1:416559/1‑139 (MQ=255)        aGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTg                                                                                                            <  2:297784/139‑1 (MQ=255)                 tgtTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACg                                                                                                                           >  2:884838/1‑117 (MQ=255)                 tgtTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACg                                                                                                                           <  1:884838/117‑1 (MQ=255)                              gTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGcgcg                                                                                                                                     >  1:1039513/1‑94 (MQ=255)                              gTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGcgcg                                                                                                                                     <  2:1039513/94‑1 (MQ=255)                              gTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTa                                                                                      <  1:415510/139‑1 (MQ=255)                                       aTTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGt                                                                             >  2:402570/1‑139 (MQ=255)                                         taataaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTg                                                                                                         >  2:937226/1‑109 (MQ=255)                                         taataaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTg                                                                                                         <  1:937226/109‑1 (MQ=255)                                         taataaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACTGGAGTGc                                                                           >  1:1071122/1‑139 (MQ=255)                                          aataaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGcc                                                                          <  2:1071122/139‑1 (MQ=255)                                          aataaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGGGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGcc                                                                          <  2:751939/139‑1 (MQ=255)                                           ataaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCg                                                                         <  2:330782/139‑1 (MQ=255)                                            taaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCTGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTg                                                                            <  2:778824/135‑1 (MQ=255)                                            taaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCTGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACAGGAGTg                                                                            >  1:778824/1‑135 (MQ=255)                                                 tCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTga                                                                                                                   <  2:283416/93‑1 (MQ=255)                                                 tCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTga                                                                                                                   >  1:283416/1‑93 (MQ=255)                                                 tCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTta                                                                   >  2:816793/1‑139 (MQ=255)                                                 tCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTta                                                                   >  1:356716/1‑139 (MQ=255)                                                 tCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTta                                                                   <  1:966477/139‑1 (MQ=255)                                                 tCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTta                                                                   <  2:416559/139‑1 (MQ=255)                                                 tCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTta                                                                   <  1:402570/139‑1 (MQ=255)                                                 tCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTta                                                                   >  1:465303/1‑139 (MQ=255)                                                  cGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGcc                                                                          >  2:628959/1‑131 (MQ=255)                                                  cGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGcc                                                                          <  1:628959/131‑1 (MQ=255)                                                  cGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGc                                                                        <  2:360241/133‑1 (MQ=255)                                                  cGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGc                                                                        >  1:360241/1‑133 (MQ=255)                                                  cGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTtac                                                                  >  1:35857/1‑139 (MQ=255)                                                    tcaatcaaGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGcgcg                                                                                                                                     <  2:500920/72‑1 (MQ=255)                                                    tcaatcaaGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGcgcg                                                                                                                                     >  1:500920/1‑72 (MQ=255)                                                          aaGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACa                                                              <  1:935956/135‑1 (MQ=255)                                                          aaGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACa                                                              >  2:935956/1‑135 (MQ=255)                                                                  aaCGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGaa                                                  <  1:816793/139‑1 (MQ=255)                                                                    cGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAAcg                                                <  2:580177/139‑1 (MQ=255)                                                                      ccATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCTGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCg                                                                         <  1:356298/112‑1 (MQ=255)                                                                      ccATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCTGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCg                                                                         >  2:356298/1‑112 (MQ=255)                                                                      ccATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGAGTGTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAAcg                                                >  1:362725/1‑139 (MQ=255)                                                                       cATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCTGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAAcgccc                                             >  1:8341/1‑139 (MQ=255)                                                                        aTTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTa                                                                                      <  2:818000/97‑1 (MQ=255)                                                                        aTTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTa                                                                                      >  1:818000/1‑97 (MQ=255)                                                                          tATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                          <  2:356716/139‑1 (MQ=255)                                                                          tATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                          <  1:483762/139‑1 (MQ=255)                                                                                   tGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACgtg                                                                                                                         >  1:888809/1‑53 (MQ=255)                                                                                   tGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACgtg                                                                                                                         <  2:888809/53‑1 (MQ=255)                                                                                   tGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTTTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTagga                                 >  1:799294/1‑139 (MQ=255)                                                                                   tGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTg                                                                            <  2:451571/96‑1 (MQ=255)                                                                                   tGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTg                                                                            >  1:451571/1‑96 (MQ=255)                                                                                       gTTTCGCCTGATGGCTTGTGTCTGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTa                             <  2:8341/139‑1 (MQ=255)                                                                                       gTTTCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTa                             <  2:465303/139‑1 (MQ=255)                                                                                          tCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGct                                                                       <  1:854672/94‑1 (MQ=255)                                                                                          tCGCCTGATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGct                                                                       >  2:854672/1‑94 (MQ=255)                                                                                                gATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTta                                                                   <  1:911770/92‑1 (MQ=255)                                                                                                gATGGCTTGTGTCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTta                                                                   >  2:911770/1‑92 (MQ=255)                                                                                                           tCCGGCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGgcggcg         >  1:1023509/1‑139 (MQ=255)                                                                                                              ggCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGt                                      <  1:191104/107‑1 (MQ=255)                                                                                                              ggCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGt                                      >  2:191104/1‑107 (MQ=255)                                                                                                              ggCACTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGt      >  1:190650/1‑139 (MQ=255)                                                                                                                  cTGAAGCGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAgg  <  2:342134/139‑1 (MQ=255)                                                                                                                    gAAGCGCGCCATGCAACGTGGTGTGAGAGTGTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAgg  <  2:362725/139‑1 (MQ=255)                                                                                                                       |                                                                                                                                        GGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGA‑‑GTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGG  >  NC_000913/1605241‑1605492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑