Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,620,207	G→A	*73* (TAG→TAA)	marB →	periplasmic mar operon regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,620,207	0	G	A	100.0%	97.2 / NA	31	*73* (TAG→TAA)	marB	periplasmic mar operon regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/14);  total (17/14)

TGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCC  >  NC_000913/1620095‑1620334                                                                                                                 |                                                                                                                                tGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCgggg                                                                                                       >  2:85235/1‑139 (MQ=255)       ttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                                 <  1:85235/139‑1 (MQ=255)         cccccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTaa                                                                                               >  2:614477/1‑139 (MQ=255)                                 tttGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCAACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGc                                                                       >  1:262781/1‑139 (MQ=255)                                 tttGATTTAAATCACATGGGTACTGACAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGc                                                                       >  2:210002/1‑139 (MQ=255)                                     aTTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCAcc                                                                   >  2:376063/1‑139 (MQ=255)                                       ttAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGc                                                                 <  2:951931/139‑1 (MQ=255)                                            tCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCAACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGcc                                                            <  2:262781/139‑1 (MQ=255)                                               caTGGGTATTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCaaa                                                         >  1:747658/1‑139 (MQ=255)                                                 tGGGTATTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  2:747658/139‑1 (MQ=255)                                                   ggTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACa                                                     <  1:614477/139‑1 (MQ=255)                                                    gTACTGACAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACAt                                                    <  1:210002/139‑1 (MQ=255)                                                          gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACa                                                     >  1:247226/1‑132 (MQ=255)                                                          gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACa                                                     <  2:247226/132‑1 (MQ=255)                                                            aGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       >  1:136648/1‑128 (MQ=255)                                                            aGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  2:136648/128‑1 (MQ=255)                                                                        gATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTc                                                                                                                          <  1:561055/49‑1 (MQ=255)                                                                        gATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTc                                                                                                                          >  2:561055/1‑49 (MQ=255)                                                                          tGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACa                                                     >  1:7224/1‑116 (MQ=255)                                                                          tGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACa                                                     <  2:7224/116‑1 (MQ=255)                                                                            cgcTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTc                                   <  2:371379/132‑1 (MQ=255)                                                                            cgcTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTc                                   >  1:371379/1‑132 (MQ=255)                                                                                   cGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCt                                                                                                     <  1:407752/59‑1 (MQ=255)                                                                                   cGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCt                                                                                                     >  2:407752/1‑59 (MQ=255)                                                                                                    aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACa                                                     >  1:498477/1‑90 (MQ=255)                                                                                                    aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACa                                                     <  2:498477/90‑1 (MQ=255)                                                                                                    aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAc    >  2:382959/1‑139 (MQ=255)                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  1:905920/86‑1 (MQ=255)                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       >  2:905920/1‑86 (MQ=255)                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAccc  >  1:235511/1‑139 (MQ=255)                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAccc  >  1:647283/1‑139 (MQ=255)                                                                                                                 |                                                                                                                                TGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCC  >  NC_000913/1620095‑1620334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑