Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,627,625 T→C L37L (TTG→CTG)  ydfG → NADP‑dependent 3‑hydroxy acid dehydrogenase, malonic semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,627,6250TC100.0% 170.6 / NA 48L37L (TTG→CTG) ydfGNADP‑dependent 3‑hydroxy acid dehydrogenase, malonic semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/27);  total (21/27)

ACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGTTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATA  >  NC_000913/1627489‑1627745
                                                                                                                                        |                                                                                                                        
acacacAAAAGCGTTGAGGAACAGGGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAGTGCATTACTCGTCGTTTTATTCTACAAGGGCATTAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTg                                                                                                                        >  2:496930/1‑139 (MQ=255)
  acacaAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCa                                                                                                                      >  2:94250/1‑139 (MQ=255)
  acacaAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCa                                                                                                                      >  2:890746/1‑139 (MQ=255)
  acacaAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCa                                                                                                                      >  2:11055/1‑139 (MQ=255)
        aaGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGtt                                                                                                                <  2:1025900/139‑1 (MQ=255)
                    aCAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAGCt                                                                                                    <  2:764685/139‑1 (MQ=255)
                        tGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGga                                                                                                >  2:457462/1‑139 (MQ=255)
                         gcgagggtCGTTTTTGTAACTGGAGCAACGGCAGGCTGTGGTGAATGCATTGCTCGTCGTTTTATTCAACAAGGGGATAAAGTTATCGCCACTGTCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                               <  2:784675/132‑1 (MQ=255)
                         gagaTGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                               <  1:11055/139‑1 (MQ=255)
                         gagaTGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                               <  1:890746/139‑1 (MQ=255)
                         gagaTGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                               <  1:457462/139‑1 (MQ=255)
                           gatgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                             <  1:195940/139‑1 (MQ=255)
                                  gTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                               >  1:122484/1‑130 (MQ=255)
                                  gTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                               <  2:122484/130‑1 (MQ=255)
                                    tttAGTAACTGGAGCAAAGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGtat                                                                                    >  1:784675/1‑139 (MQ=255)
                                         tAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGc                                                                               <  1:573832/139‑1 (MQ=255)
                                           aCTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGa                                                                                                          <  1:506855/110‑1 (MQ=255)
                                           aCTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGa                                                                                                          >  2:506855/1‑110 (MQ=255)
                                           aCTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGccc                                                                             >  1:408266/1‑139 (MQ=255)
                                              ggAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAAc                                                                          >  1:1012087/1‑139 (MQ=255)
                                              ggAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAAc                                                                          >  1:674224/1‑139 (MQ=255)
                                                 gCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg                                                                       <  2:674224/139‑1 (MQ=255)
                                                 gCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg                                                                       <  2:1012087/139‑1 (MQ=255)
                                                     cGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGt                                                                   >  1:191172/1‑139 (MQ=255)
                                                     cGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGt                                                                   >  2:548646/1‑139 (MQ=255)
                                                                 gTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACcgcgt                                                       <  1:94250/139‑2 (MQ=255)
                                                                      tGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                             <  1:807758/96‑1 (MQ=255)
                                                                      tGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                             >  2:807758/1‑96 (MQ=255)
                                                                       gCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg                                                     <  2:23828/135‑1 (MQ=255)
                                                                       gCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg                                                     >  1:23828/1‑135 (MQ=255)
                                                                           tACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAg                                                                                                                     >  1:828817/1‑67 (MQ=255)
                                                                           tACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAg                                                                                                                     <  2:828817/67‑1 (MQ=255)
                                                                           tACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGaa                                             <  2:191172/139‑1 (MQ=255)
                                                                             cTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAaga                                           <  1:548646/139‑1 (MQ=255)
                                                                                gtcgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGAt                                        >  1:576402/1‑139 (MQ=255)
                                                                                gtcgtTTAATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGAAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACGGCGCCGCTATTGAAGAGAt                                        >  2:727712/1‑139 (MQ=255)
                                                                                                      tAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAg                  <  1:930102/139‑1 (MQ=255)
                                                                                                             ttCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                             <  1:863443/56‑1 (MQ=255)
                                                                                                             ttCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                             >  2:863443/2‑57 (MQ=255)
                                                                                                             aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg                                                                       <  2:502845/79‑1 (MQ=255)
                                                                                                             aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg                                                                       >  1:502845/1‑79 (MQ=255)
                                                                                                             aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCgcgc                                                       <  2:502281/95‑1 (MQ=255)
                                                                                                             aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCgcgc                                                       >  1:502281/1‑95 (MQ=255)
                                                                                                              tCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCaa          <  2:408266/139‑1 (MQ=255)
                                                                                                                gCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAata        <  1:283139/139‑1 (MQ=255)
                                                                                                                   aCTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTg     <  2:367180/139‑1 (MQ=255)
                                                                                                                      ggCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGata  <  1:221367/139‑1 (MQ=255)
                                                                                                                       gCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                             >  2:538819/1‑47 (MQ=255)
                                                                                                                       gCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                             <  1:538819/47‑1 (MQ=255)
                                                                                                                                        |                                                                                                                        
ACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGTTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATA  >  NC_000913/1627489‑1627745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑