Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,655,057	T→C	T29A (ACG→GCG)	rspA ←	bifunctional D‑altronate/D‑mannonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,655,057	0	T	C	100.0%	121.2 / NA	35	T29A (ACG→GCG)	rspA	bifunctional D‑altronate/D‑mannonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/19);  total (16/19)

CACCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACT  >  NC_000913/1654922‑1655189                                                                                                                                        |                                                                                                                                     cACCTTTATAGAAAAACTGCCAGATATCGTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAAt                                                                                                                                   <  2:593880/139‑1 (MQ=255)    cTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATAcc                                                                                                                                <  1:81572/139‑1 (MQ=255)    cTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATAcc                                                                                                                                >  2:863476/1‑139 (MQ=255)      ttATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGt                                                                                                                              >  1:946876/1‑139 (MQ=255)       tataGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTc                                                                                                                             <  2:946876/139‑1 (MQ=255)                aCTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCATCCTCAgtggt                                                                                                                    <  1:22297/139‑1 (MQ=255)                                     ggTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                               <  1:497049/139‑1 (MQ=255)                                     ggTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                               >  2:866578/1‑139 (MQ=255)                                      gTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTa                                                                                              >  1:428637/1‑139 (MQ=255)                                         cgcATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGc                                                                                           >  1:1003258/1‑139 (MQ=255)                                          gcATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGcc                                                                                          >  2:67279/1‑139 (MQ=255)                                                             gCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAAc                                                                       >  1:131411/1‑139 (MQ=255)                                                              cGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACt                                                                      >  2:258478/1‑139 (MQ=255)                                                              cGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACt                                                                      <  1:866578/139‑1 (MQ=255)                                                                 aCAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCa                                                                   <  1:258478/139‑1 (MQ=255)                                                                          aTCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACg                                                          >  1:122537/1‑139 (MQ=255)                                                                               gCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACtt                                                                     <  2:811750/123‑1 (MQ=255)                                                                               gCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACtt                                                                     >  1:811750/1‑123 (MQ=255)                                                                               gCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAg                                                                  >  1:882051/1‑126 (MQ=255)                                                                               gCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAg                                                                  <  2:882051/126‑1 (MQ=255)                                                                                          gCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGAtt                                          >  2:52243/1‑139 (MQ=255)                                                                                                 agtgCTCACGTCCATTGACGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCa                                   <  2:55684/136‑1 (MQ=255)                                                                                                     cTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGc                               <  2:122537/139‑1 (MQ=255)                                                                                                        aCGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCa                                   >  2:961250/1‑132 (MQ=255)                                                                                                        aCGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCa                                   <  1:961250/132‑1 (MQ=255)                                                                                                              aTTGAGGGTGGCATCCCCAAGGCCCGCCATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCCGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATTTTCGATTCCTTGCATCGCTTGTCGTGa                      <  2:552386/139‑1 (MQ=255)                                                                                                                    ggTGGCATCCCCAAGGCCCGCAATACCGTCCTCAg                                                                                                                        <  1:931478/35‑1 (MQ=38)                                                                                                                    ggTGGCATCCCCAAGGCCCGCAATACCGTCCTCAg                                                                                                                        >  2:931478/1‑35 (MQ=38)                                                                                                                        gCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTTCTTGCATCGCTTGTCGTGATGCATGAAAt            >  1:9309/1‑139 (MQ=255)                                                                                                                           tCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTa         <  2:864275/139‑1 (MQ=255)                                                                                                                            ccccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTa         <  2:18405/138‑1 (MQ=255)                                                                                                                            ccccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTa         >  1:18405/1‑138 (MQ=255)                                                                                                                                aaGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCaa    <  2:115936/139‑1 (MQ=255)                                                                                                                                 aGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAAc   <  1:494703/139‑1 (MQ=255)                                                                                                                                  ggCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACt  <  1:67279/139‑1 (MQ=255)                                                                                                                                  ggCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACt  <  1:52243/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     CACCTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACT  >  NC_000913/1654922‑1655189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑