Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	157,713	G→A	A7V (GCC→GTC)	folK ←	2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	157,713	0	G	A	100.0%	104.1 / NA	34	A7V (GCC→GTC)	folK	2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/18);  total (16/18)

TTGATCTTGCGGCCCCAGCGGTGGGGTGCGGTAAAACGAAGAAACGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGGCAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACGGTTCTTCATCCAGCTCGTTGAGCATCCCTTTTTGGTCTGGTGGCGCG  >  NC_000913/157580‑157840                                                                                                                                      |                                                                                                                                ttGATCTTGCGGCCCCAGCGGTGGGGTGCGGTAAAACGAAGAAACGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAata                                                                                                                            <  2:623223/139‑1 (MQ=255) ttGATCTTGCGGCCCCAGCGGTGGGGTGCGGTAAAACGAAGAAACGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAata                                                                                                                            <  1:967935/139‑1 (MQ=255)          cGGCCCCAGCGGTGGGGTGCGGTAAAACGAAGAAACGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCa                                                                                                                     >  1:317562/1‑137 (MQ=255)          cGGCCCCAGCGGTGGGGTGCGGTAAAACGAAGAAACGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCa                                                                                                                     <  2:317562/137‑1 (MQ=255)                cAGCGGTGGGGTGCGGTAAAACGAAGAAACGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATg                                                                                                             <  1:945183/139‑1 (MQ=255)                 aGCGGTGGGGTGCGGTAAAACGAAGAAACGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGc                                                                                                            <  2:837625/139‑1 (MQ=255)                 aGCGGTGGGGTGCGGTAAAACGAAGAAACGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGc                                                                                                            >  2:481782/1‑139 (MQ=255)                     gTGGGGTGCGGTAAAACGAAGAAACGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTa                                                                                                        <  2:674959/139‑1 (MQ=255)                             cGGTAAAACGAAGAAACGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCacg                                                                                                <  1:481782/139‑1 (MQ=255)                                          aaaCGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGt                                                                                   >  2:994684/1‑139 (MQ=255)                                              ggTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGc                                                                               >  1:531612/1‑139 (MQ=255)                                                  aGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTc                                                                                                                >  2:1023248/1‑102 (MQ=255)                                                  aGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTc                                                                                                                <  1:1023248/102‑1 (MQ=255)                                                    aaTGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCgg                                                                                                          <  1:43231/106‑1 (MQ=255)                                                    aaTGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCgg                                                                                                          >  2:43231/1‑106 (MQ=255)                                                     aggtgGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCTGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTAgacg                                                                        <  2:655851/137‑1 (MQ=255)                                                                     tatCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGAcgc                                                        >  2:815459/1‑139 (MQ=255)                                                                          ccTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGa                                                   <  1:815459/139‑1 (MQ=255)                                                                               tGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACGGtt                                              <  1:762691/139‑1 (MQ=255)                                                                                   ttCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGTCGACGAGTACGACGAGGCTGTGACGGttcttc                                          >  2:688512/1‑139 (MQ=255)                                                                                         gcagcaTTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACg                                                 <  2:247343/126‑1 (MQ=255)                                                                                         gcagcaTTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACg                                                 >  1:247343/1‑126 (MQ=255)                                                                                             caTTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTc                                                                                                   >  2:776230/1‑72 (MQ=255)                                                                                             caTTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTc                                                                                                   <  1:776230/72‑1 (MQ=255)                                                                                                    cTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACGGTTCTTCATCCAGCTCGTTGAGCa                         <  1:994684/139‑1 (MQ=255)                                                                                                       cTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGTCGGTTCTTCATCCAGCTCGTTGAGCATcc                      >  2:717272/1‑139 (MQ=255)                                                                                                       cTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACGGTTCTTCATCCAGCTCGTTGAGCATcc                      >  2:1056682/1‑139 (MQ=255)                                                                                                       cTCCAGCGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACGGTTCTTCATCCAGCTCGTTGAGCATcc                      >  1:10711/1‑139 (MQ=255)                                                                                                             cGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACg                                                 <  2:1059353/106‑1 (MQ=255)                                                                                                             cGGAGAGGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACg                                                 >  1:1059353/1‑106 (MQ=255)                                                                                                                 gagGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACGGTTCTTCATCCAGCTCGTTGAGCATCCCTTTTTGGTc            >  1:519390/1‑139 (MQ=255)                                                                                                                  agGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGt                                                     <  2:753857/97‑1 (MQ=255)                                                                                                                  agGCCAGATTGCTGCCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGt                                                     >  1:753857/1‑97 (MQ=255)                                                                                                                           tgctgcCTATGACAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACGGTTCTTCATCCAGCTCGTTGAGCATCCCTTTTTGGTCTGGTGGcgcg  <  2:531612/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                TTGATCTTGCGGCCCCAGCGGTGGGGTGCGGTAAAACGAAGAAACGGTAAGAATGTGGCTTTCAGGGATATCGCCTAATGCTTTCAGGGCAGCATTGACCTGCTCCAGCGGAGAGGCCAGATTGCTGCCTATGGCAATATACGCCACTGTCATGCGGTACCCTCACGACGTGGTGCGCGTTTGCGTGGACGACGAGTACGACGACGCGGTGACGGTTCTTCATCCAGCTCGTTGAGCATCCCTTTTTGGTCTGGTGGCGCG  >  NC_000913/157580‑157840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑