Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,301	C→T	A45V (GCG→GTG)	dnaJ →	chaperone Hsp40, DnaK co‑chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,301	0	C	T	100.0%	184.8 / NA	58	A45V (GCG→GTG)	dnaJ	chaperone Hsp40, DnaK co‑chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

TAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGG  >  NC_000913/14173‑14409                                                                                                                                 |                                                                                                             tAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCGCCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTaaa                                                                                                    <  1:140691/139‑1 (MQ=255)         attattACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTaa                                                                                                     <  2:969662/130‑1 (MQ=255)         attattACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTaa                                                                                                     >  1:969662/1‑130 (MQ=255)                   gaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTAt                                                                                  >  2:357709/1‑139 (MQ=255)                    aTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                 <  1:357709/139‑1 (MQ=255)                                tcaaaaaCAGCGAAAGAGCGTGAAATCAGACAGGCCTACAAACGCCTCGCCATGAAATCACACCCGGACCGTAACCGGGGTGACAAAGAGCCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCg                                                                     >  2:710270/4‑139 (MQ=255)                                          cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTaa                                                                                                     >  2:302575/1‑97 (MQ=255)                                          cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTaa                                                                                                     <  1:302575/97‑1 (MQ=255)                                          cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                           >  2:628314/1‑139 (MQ=255)                                          cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                           >  2:354821/1‑139 (MQ=255)                                          cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                           >  2:725791/1‑139 (MQ=255)                                          cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                           >  1:530351/1‑139 (MQ=255)                                               gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                      <  1:354821/139‑1 (MQ=255)                                               gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                      <  1:628314/139‑1 (MQ=255)                                                agCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                     <  2:11819/139‑1 (MQ=255)                                                agCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                     <  1:725791/139‑1 (MQ=255)                                                    tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTaaa                                                                                                    <  1:579635/88‑1 (MQ=255)                                                    tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTaaa                                                                                                    >  2:579635/1‑88 (MQ=255)                                                    tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCa                                                 >  1:576869/1‑139 (MQ=255)                                                       aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCGGGGTGACAAAGAGGCCGAGGTGAAATTTaaa                                                                                                    <  2:217073/85‑1 (MQ=255)                                                       aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCGGGGTGACAAAGAGGCCGAGGTGAAATTTaaa                                                                                                    >  1:217073/1‑85 (MQ=255)                                                        aTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                             <  2:630812/139‑1 (MQ=255)                                                          cAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                 <  1:477643/101‑1 (MQ=255)                                                          cAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                 >  2:477643/1‑101 (MQ=255)                                                                    tACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCa                                                 <  2:94633/123‑1 (MQ=255)                                                                    tACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCa                                                 >  1:94633/1‑123 (MQ=255)                                                                           gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGa                                                                                         <  1:166742/76‑1 (MQ=255)                                                                           gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGa                                                                                         >  2:166742/1‑76 (MQ=255)                                                                           gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGa                                                                    <  1:877374/97‑1 (MQ=255)                                                                           gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGa                                                                    >  2:877374/1‑97 (MQ=255)                                                                             cTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGTGGCATACGATCAGTATGGTCATGCTGCGt                        <  2:1094259/139‑1 (MQ=255)                                                                                gCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGa                                                                    <  2:430444/92‑1 (MQ=255)                                                                                gCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGa                                                                    >  1:430444/1‑92 (MQ=255)                                                                                 ccATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGa                    <  2:530351/139‑1 (MQ=255)                                                                                     gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTaaa                                                                                                    <  1:833212/55‑1 (MQ=255)                                                                                     gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTaaa                                                                                                    >  2:833212/1‑55 (MQ=255)                                                                                               ccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                        <  2:166070/121‑1 (MQ=255)                                                                                               ccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                        >  1:166070/1‑121 (MQ=255)                                                                                               ccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCa                 <  1:7637/128‑1 (MQ=255)                                                                                               ccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCa                 >  2:7637/1‑128 (MQ=255)                                                                                               ccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATgg      >  1:892221/1‑139 (MQ=255)                                                                                                   aCCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcgg  >  1:1047083/1‑139 (MQ=255)                                                                                                      gTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTa                                                                                                      >  2:355948/1‑36 (MQ=255)                                                                                                      gTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTa                                                                                                      <  1:355948/36‑1 (MQ=255)                                                                                                        aaCCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                             >  2:272433/1‑91 (MQ=255)                                                                                                        aaCCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                             <  1:272433/91‑1 (MQ=255)                                                                                                        aaCCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                           >  2:102436/1‑93 (MQ=255)                                                                                                        aaCCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                           <  1:102436/93‑1 (MQ=255)                                                                                                               gTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGt                                  >  2:507453/1‑95 (MQ=255)                                                                                                               gTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGt                                  <  1:507453/95‑1 (MQ=255)                                                                                                                        agGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAg                                        <  2:467344/80‑1 (MQ=255)                                                                                                                        agGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAg                                        >  1:467344/1‑80 (MQ=255)                                                                                                                         ggCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                     <  2:208868/66‑1 (MQ=255)                                                                                                                         ggCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                     >  1:901771/1‑66 (MQ=255)                                                                                                                         ggCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                     <  2:901771/66‑1 (MQ=255)                                                                                                                         ggCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                     >  1:208868/1‑66 (MQ=255)                                                                                                                         ggCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGt                                  <  2:251717/85‑1 (MQ=255)                                                                                                                         ggCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGt                                  >  1:251717/1‑85 (MQ=255)                                                                                                                                 |                                                                                                             TAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGG  >  NC_000913/14173‑14409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑