Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,757,721 C→T *335* (TAG→TAA ldtE ← murein L,D‑transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,7210CT100.0% 101.8 / NA 33*335* (TAG→TAAldtEmurein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/14);  total (19/14)

GCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTG  >  NC_000913/1757589‑1757839
                                                                                                                                    |                                                                                                                      
gctgctAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGc                                                                                                                  >  1:408841/1‑139 (MQ=255)
          aTGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTa                                                                                                        <  2:670403/139‑1 (MQ=255)
                                  gTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattc                                                                                >  1:546395‑M1/1‑130 (MQ=255)
                                                      aaaTACCGCAAGTAATGGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCGTTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCTCTTGCTtcaccattctgcgctgactctactgaagg                                                            >  1:277167‑M1/1‑110 (MQ=255)
                                                              cAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgc                                                    >  1:322116‑M1/1‑102 (MQ=255)
                                                              cAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgc                                                    >  2:1049879‑M1/1‑102 (MQ=255)
                                                                         aCCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgc                                                                             <  2:822499‑M1/103‑13 (MQ=255)
                                                                         aCCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgc                                                                             >  1:822499‑M1/1‑91 (MQ=255)
                                                                          ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcactgactctactgaaggcgcattgctggctgcgggag                                        >  2:691243‑M1/1‑90 (MQ=255)
                                                                           cTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg                                                                                                          <  2:422720/72‑1 (MQ=255)
                                                                           cTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg                                                                                                          >  1:422720/1‑72 (MQ=255)
                                                                            tgtgAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTccc                                                                                              >  2:1095777/1‑83 (MQ=255)
                                                                            tgtgAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTccc                                                                                              <  1:1095777/83‑1 (MQ=255)
                                                                             gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGt                                                                                                                >  2:732081/1‑64 (MQ=255)
                                                                             gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGt                                                                                                                <  1:732081/64‑1 (MQ=255)
                                                                             gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTc                                                                                                               >  2:344088/1‑65 (MQ=255)
                                                                             gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTc                                                                                                               <  1:344088/65‑1 (MQ=255)
                                                                             gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttg                                     >  2:162167‑M1/1‑87 (MQ=255)
                                                                              tgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGt                                                                                                                <  1:17789/63‑1 (MQ=255)
                                                                              tgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGt                                                                                                                >  2:17789/1‑63 (MQ=255)
                                                                                     aaaaaTGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactg                             >  2:412105‑M1/1‑79 (MQ=255)
                                                                                            gcgcACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattccgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccg                      <  1:956368‑M1/139‑68 (MQ=255)
                                                                                                    tgtgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTcc                                                                                               >  2:43659/1‑58 (MQ=255)
                                                                                                    tgtgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTcc                                                                                               <  1:43659/58‑1 (MQ=255)
                                                                                                      tgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggata            >  1:498455‑M1/1‑62 (MQ=255)
                                                                                                      tgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                          <  2:866063‑M1/77‑16 (MQ=255)
                                                                                                      tgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                          >  1:866063‑M1/1‑62 (MQ=255)
                                                                                                           cATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcaatgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctg       <  1:691243‑M1/139‑83 (MQ=255)
                                                                                                                tttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTc                                                                                                >  2:172184/1‑45 (MQ=255)
                                                                                                                tttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTc                                                                                                <  1:172184/45‑1 (MQ=255)
                                                                                                                tttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcccga  <  1:162167‑M1/139‑88 (MQ=255)
                                                                                                                tttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgct                                                   >  2:452867‑M1/1‑52 (MQ=255)
                                                                                                                tttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgct                                                   <  1:452867‑M1/90‑39 (MQ=255)
                                                                                                                                    |                                                                                                                      
GCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTG  >  NC_000913/1757589‑1757839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: