Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,841,897 G→A *136* (TAG→TAA
T80I (ACT→ATT) 
nudG →
ynjH ←
CTP pyrophosphohydrolase, also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
DUF1496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,841,8970GA100.0% 115.1 / NA 36*136* (TAG→TAA
T80I (ACT→ATT) 
nudG
ynjH
CTP pyrophosphohydrolase, also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
DUF1496 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/19);  total (17/19)

CATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGA  >  NC_000913/1841775‑1842027
                                                                                                                          |                                                                                                                                  
caTGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtc                                                                                                                    >  1:1022961/1‑139 (MQ=255)
 aTGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtca                                                                                                                   >  1:1088628/1‑139 (MQ=255)
 aTGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtca                                                                                                                   >  2:1036724/1‑139 (MQ=255)
  tGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtcat                                                                                                                  >  1:900835/1‑139 (MQ=255)
           ggCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGc                                                                                                         >  2:412113/1‑139 (MQ=255)
               ctggtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTg                                                                                                     <  2:900835/139‑1 (MQ=255)
                tggtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGt                                                                                                                          >  2:495073/1‑117 (MQ=255)
                tggtctggtGCACACCTGAAGAGGCGCTGCAATATCCGCTGGCCACTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGt                                                                                                                          <  1:495073/117‑1 (MQ=255)
                 ggtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATTGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgca                                                                                                   <  2:724786/139‑1 (MQ=255)
                  gtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcag                                                                                                  <  2:923380/139‑1 (MQ=255)
                            cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcag                                                                                                  <  2:4230/129‑1 (MQ=255)
                            cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcag                                                                                                  >  1:4230/1‑129 (MQ=255)
                                  aaGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCt                                                                                  <  1:862129/139‑1 (MQ=255)
                                         gcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTa                                                                           >  1:602611/1‑139 (MQ=255)
                                             cAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGc                                                                       <  2:1088628/139‑1 (MQ=255)
                                             cAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGc                                                                       <  1:411809/139‑1 (MQ=255)
                                                  tCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTa                                                                                             >  1:83058/1‑112 (MQ=255)
                                                  tCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTa                                                                                             <  2:83058/112‑1 (MQ=255)
                                                  tCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCtt                                                                  <  1:105184/139‑1 (MQ=255)
                                                  tCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCtt                                                                  <  1:264359/139‑1 (MQ=255)
                                                                      ttCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGa                                              >  2:852106/1‑139 (MQ=255)
                                                                                 gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcagca                                   <  2:602611/139‑1 (MQ=255)
                                                                                   ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCtt                                                                  >  1:580963/1‑106 (MQ=255)
                                                                                   ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCtt                                                                  <  2:580963/106‑1 (MQ=255)
                                                                                   ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACt                                 <  2:628813/139‑1 (MQ=255)
                                                                                     cGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                       <  1:718681/83‑1 (MQ=255)
                                                                                     cGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTATGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                       >  2:718681/1‑83 (MQ=255)
                                                                                       tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCa                                                                                                        >  2:218242/1‑64 (MQ=255)
                                                                                       tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCa                                                                                                        <  1:218242/64‑1 (MQ=255)
                                                                                          aTGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACa                          <  1:852106/139‑1 (MQ=255)
                                                                                                ttACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTg                                                                                                     >  1:7134/1‑58 (MQ=255)
                                                                                                ttACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTg                                                                                                     <  2:7134/58‑1 (MQ=255)
                                                                                                 tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgc                   >  2:769391/1‑139 (MQ=255)
                                                                                                        cgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgc                                                                                                    >  2:173196/1‑51 (MQ=255)
                                                                                                        cgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgc                                                                                                    <  1:173196/51‑1 (MQ=255)
                                                                                                                  gCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctga  >  2:275242/1‑139 (MQ=255)
                                                                                                                          |                                                                                                                                  
CATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGA  >  NC_000913/1841775‑1842027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: