Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,926,096	G→A	*219* (TAG→TAA)	yobB →	C‑N hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,096	0	G	A	100.0%	184.3 / NA	58	*219* (TAG→TAA)	yobB	C‑N hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/27);  total (31/27)

TAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT  >  NC_000913/1925976‑1926229                                                                                                                         |                                                                                                                                      tAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGc                                                                                                                     <  1:158208/139‑1 (MQ=255)    cAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                  >  2:37633/1‑139 (MQ=255)      gTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           >  2:189556/1‑128 (MQ=255)      gTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  1:189556/128‑1 (MQ=255)         cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                             <  2:241607/139‑1 (MQ=255)         cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                             >  1:241607/1‑139 (MQ=255)          aCTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGt                                                                                                            >  1:1047466/1‑139 (MQ=255)               gggATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                       <  1:928496/139‑1 (MQ=255)                 gATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAt                                                                                                     >  1:262735/1‑139 (MQ=255)                   tGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           >  2:606008/1‑115 (MQ=255)                   tGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  1:606008/115‑1 (MQ=255)                        atGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                       >  1:1017993/1‑130 (MQ=255)                        atGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                       <  2:1017993/130‑1 (MQ=255)                               tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGaaa                                                                                       >  1:108595/1‑139 (MQ=255)                                ctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                      >  2:996379/1‑139 (MQ=255)                                ctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                      >  1:384141/1‑139 (MQ=255)                                     cGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCg                                                                                 <  2:1047466/139‑1 (MQ=255)                                        tCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGtt                                                                              >  2:1085044/1‑139 (MQ=255)                                                 tCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggag                                                                     <  1:1085044/139‑1 (MQ=255)                                                  cGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggagg                                                                    <  1:385360/139‑1 (MQ=255)                                                              ttGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt                                                        <  2:102357/139‑1 (MQ=255)                                                                 ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAg                                                                                                                      <  1:344275/74‑1 (MQ=255)                                                                 ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAg                                                                                                                      >  2:344275/1‑74 (MQ=255)                                                                   aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                  <  1:236653/92‑1 (MQ=255)                                                                   aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                  >  2:236653/1‑92 (MQ=255)                                                                   aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   >  2:509162/1‑139 (MQ=255)                                                                    gTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                >  2:250581/1‑77 (MQ=255)                                                                    gTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                <  1:250581/77‑1 (MQ=255)                                                                      cGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATCCCATTACGCTAAGCGTTTTGCGACTGGAGCATGTCATGTTGCGCATTATCGATACAGAAACCTGCGGTGTGCAGGGAGGGTACGTTGATATTGCCTCTg                                                >  2:188353/1‑139 (MQ=255)                                                                        gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGtt                                              >  2:119262/1‑139 (MQ=255)                                                                         tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGC‑TTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGa                                            >  1:351373/1‑139 (MQ=255)                                                                                  atcaCAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGC‑TTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGa                                   <  2:351373/139‑1 (MQ=255)                                                                                         ggTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaa                             >  1:229003/1‑139 (MQ=255)                                                                                            tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                >  1:1095266/1‑53 (MQ=255)                                                                                            tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                <  2:1095266/53‑1 (MQ=255)                                                                                            tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAt                                                                                                     >  2:27236/1‑64 (MQ=255)                                                                                            tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAt                                                                                                     <  1:27236/64‑1 (MQ=255)                                                                                            tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAt                          >  2:137140/1‑139 (MQ=255)                                                                                              gCAAGGCGATATCATTCCATTACGCTAATCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   <  1:591380/112‑1 (MQ=255)                                                                                              gCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           >  2:1087101/1‑40 (MQ=255)                                                                                              gCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  1:1087101/40‑1 (MQ=255)                                                                                              gCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   >  2:591380/1‑112 (MQ=255)                                                                                                  ggCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCaa                    <  2:229003/139‑1 (MQ=255)                                                                                                     gATATCATTCCATTACGCTAAGCTTTTTCGGCCTgg                                                                                                                        >  1:425680/1‑36 (MQ=255)                                                                                                     gATATCATTCCATTACGCTAAGCTTTTTCGGCCTgg                                                                                                                        <  2:425680/36‑1 (MQ=255)                                                                                                      atatCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAccac                <  2:384141/139‑3 (MQ=255)                                                                                                        atCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTg                                                                            >  1:558341/1‑77 (MQ=255)                                                                                                        atCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTg                                                                            <  2:558341/77‑1 (MQ=255)                                                                                                          cATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGa            <  1:996379/139‑1 (MQ=255)                                                                                                             tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGc                                                                                  <  2:138587/66‑1 (MQ=255)                                                                                                             tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGc                                                                                  >  1:138587/1‑66 (MQ=255)                                                                                                             tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGcc         >  2:575509/1‑139 (MQ=255)                                                                                                                 ttACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGTCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCt     <  1:1037437/139‑1 (MQ=255)                                                                                                                 ttACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAAAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAATCCCATGAGCCACCt     >  2:1037437/1‑139 (MQ=255)                                                                                                                   aCGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTgg   <  1:37633/139‑1 (MQ=255)                                                                                                                    cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGa                                                                                         >  1:250047/1‑52 (MQ=255)                                                                                                                    cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGa                                                                                         <  2:250047/52‑1 (MQ=255)                                                                                                                    cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt  >  1:838235/1‑139 (MQ=255)                                                                                                                         |                                                                                                                                      TAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT  >  NC_000913/1925976‑1926229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑