Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,931,373 C→T Q165* (CAG→TAG)  purT → phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,931,3730CT100.0% 73.6 / NA 24Q165* (CAG→TAG) purTphosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/10);  total (14/10)

CAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAAC  >  NC_000913/1931256‑1931503
                                                                                                                     |                                                                                                                                  
cagctgcCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCag                                                                                                               >  2:551891/1‑139 (MQ=255)
cagctgcCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTAGCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCag                                                                                                               <  2:833983/139‑1 (MQ=255)
            tccacttATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTc                                                                                                   <  2:1030718/139‑1 (MQ=255)
              cacttATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAg                                                                                                 <  1:763133/139‑1 (MQ=255)
                 ttATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCa                                                                                              <  2:378628/139‑1 (MQ=255)
                     cGTTTTGCCGATAGGGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGa                                                                                          >  2:1101869/1‑139 (MQ=255)
                                             ttCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAg                                                                                                                                  >  2:25514/1‑75 (MQ=255)
                                                            gCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCcgcg                                                   <  1:1101869/139‑1 (MQ=255)
                                                                          tCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGc                                     >  1:548453/1‑139 (MQ=255)
                                                                          tCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGc                                     >  1:615493/1‑139 (MQ=255)
                                                                          tCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGc                                     >  2:1006736/1‑139 (MQ=255)
                                                                              tGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGcgtcg                                 <  1:551891/139‑1 (MQ=255)
                                                                                cATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTATTGAAGGCGTCGtt                               >  2:650025/1‑139 (MQ=255)
                                                                                     tAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGc                                                       <  2:878637/110‑1 (MQ=255)
                                                                                     tAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGc                                                       >  1:878637/1‑110 (MQ=255)
                                                                                     tAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGtt                          >  2:86242/1‑139 (MQ=255)
                                                                                     tAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTCTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGtt                          >  1:781386/1‑139 (MQ=255)
                                                                                           cGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACtt                    >  1:34523/1‑139 (MQ=255)
                                                                                               gatgaGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAg                                                                           >  1:658024/1‑80 (MQ=255)
                                                                                               gatgaGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAg                                                                           <  2:658024/80‑1 (MQ=255)
                                                                                                     ctctTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTCACTTCGAAATTAcc          >  1:680751/1‑139 (MQ=255)
                                                                                                               aaGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCggg                                                        >  2:540454/1‑83 (MQ=255)
                                                                                                               aaGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCggg                                                        <  1:540454/83‑1 (MQ=255)
                                                                                                                  gggTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAAc  <  2:186194/134‑1 (MQ=255)
                                                                                                                     |                                                                                                                                  
CAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAAC  >  NC_000913/1931256‑1931503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: