Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,942,993 | G→A | G111E (GGG→GAG) | znuC → | zinc ABC transporter ATPase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,942,993 | 0 | G | A | 97.9% | 147.1 / NA | 48 | G111E (GGG→GAG) | znuC | zinc ABC transporter ATPase |
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (27/20); minor base C (1/0); total (28/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGGGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTA > NC_000913/1942881‑1943120 | cAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCaaa > 1:962968/1‑139 (MQ=255) aaGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGc > 1:514714/1‑139 (MQ=255) gCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGctc > 1:154118/1‑139 (MQ=255) gTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGc > 2:435132/1‑107 (MQ=255) gTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGc < 1:435132/107‑1 (MQ=255) gACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCg > 1:699943/1‑139 (MQ=255) ccacGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAAc > 2:565407/1‑139 (MQ=255) cacGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACg > 2:943874/1‑139 (MQ=255) ttGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGc < 1:943874/139‑1 (MQ=255) ccACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg < 2:154118/139‑1 (MQ=255) ccAATGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACCCAAAAAGAAAATATTTTGCCTGCCCTGAAACGTTTCCAGGCCGAGCCCCTGATTAACGCCCCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg < 2:962968/139‑1 (MQ=255) tAAACCGTTTTTTACGCTTACGCCCTGGTCCACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTACCGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGc > 1:404445/1‑123 (MQ=255) tAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGc < 2:404445/123‑1 (MQ=255) tAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAgc > 1:495009/1‑139 (MQ=255) gTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa < 2:1054211/118‑1 (MQ=255) gTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa > 1:1054211/1‑118 (MQ=255) ttacgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGc < 1:725719/109‑1 (MQ=255) ttacgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGc > 2:725719/1‑109 (MQ=255) ttacgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATc < 2:514714/139‑1 (MQ=255) cTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCa < 1:866739/139‑1 (MQ=255) gTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAatt > 2:701334/1‑139 (MQ=255) acacATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAattat < 2:495009/139‑1 (MQ=255) acacATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAattat < 2:236178/139‑1 (MQ=255) aTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGc > 1:303949/1‑112 (MQ=255) aTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGc < 2:303949/112‑1 (MQ=255) aTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGt > 2:878877/1‑139 (MQ=255) agaagaTATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTg > 2:673585/1‑139 (MQ=255) atatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCaa < 1:1054039/59‑1 (MQ=255) atatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCaa > 2:1054039/1‑59 (MQ=255) tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGaa > 2:1048183/1‑139 (MQ=255) atTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAAc < 1:1048183/139‑1 (MQ=255) ccTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGccc < 1:477568/110‑1 (MQ=255) ccTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGccc > 2:477568/1‑110 (MQ=255) gCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAg > 1:823291/1‑139 (MQ=255) cACTGAAACGTGTCCAGGTCGAGCACCTGATTAATGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg > 2:618342/1‑75 (MQ=255) cACTGAAACGTGTCCAGGCCGAGCACCTGATTAATGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg < 1:618342/75‑1 (MQ=255) tgtCCAGGCCGCGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTa > 2:877877/1‑139 (MQ=255) tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg < 2:383773/65‑1 (MQ=255) tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg > 1:383773/1‑65 (MQ=255) tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCg < 2:310032/63‑1 (MQ=255) tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCg > 1:310032/1‑63 (MQ=255) tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAAt > 2:46045/1‑91 (MQ=255) tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAAt < 1:46045/91‑1 (MQ=255) tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGGTGAACCAACTCAAGGCGTGGCTGTa > 2:491162/1‑139 (MQ=255) tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTa > 2:50298/1‑139 (MQ=255) tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTa > 2:423655/1‑139 (MQ=255) gtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg < 2:277677/60‑1 (MQ=255) gtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg > 1:277677/1‑60 (MQ=255) | CAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGGGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTA > NC_000913/1942881‑1943120 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |