| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 182,006 | A→G | N375D (AAC→GAC) | degP → | serine endoprotease (protease Do), membrane‑associated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 182,006 | 0 | A | G | 97.9% | 160.9 / ‑4.7 | 47 | N375D (AAC→GAC) | degP | serine endoprotease (protease Do), membrane‑associated |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (25/21); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
TGTGATCACCTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGAACAACGTGAAAA > NC_000913/181885‑182135 | tgtgatCACCTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTg > 1:785960/1‑139 (MQ=255) tgatCACCTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTgca < 1:13315/139‑1 (MQ=255) cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGTGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCagcagagc > 1:97953/1‑139 (MQ=255) cTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCagcagagc > 2:1010196/1‑139 (MQ=255) tGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTAACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCa > 2:12021/1‑139 (MQ=255) ggTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATc > 1:1083844/1‑139 (MQ=255) ggTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATc > 2:201205/1‑139 (MQ=255) tCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTc < 2:831377/139‑1 (MQ=255) gCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTAACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCa < 1:12021/139‑1 (MQ=255) aCTGTGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAAc < 2:97953/139‑1 (MQ=255) cGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCa > 2:790113/1‑139 (MQ=255) tCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCagcagag < 2:682974/93‑1 (MQ=255) acAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGTCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCagcagag > 1:682974/2‑93 (MQ=255) cAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAg < 1:790113/139‑1 (MQ=255) cAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCACCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAg < 2:787558/139‑1 (MQ=255) ggTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCGTTGa > 1:660378/1‑135 (MQ=255) ggTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGgc > 2:708940/1‑139 (MQ=255) ggTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCACGTTGATTCCAGCTCCATCTTCAACGGCATTGa < 2:660378/135‑1 (MQ=255) tACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACg > 1:338019/1‑122 (MQ=255) tACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACg < 2:338019/122‑1 (MQ=255) tATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATg > 1:569985/1‑139 (MQ=255) tGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGACCTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAg > 2:813029/1‑139 (MQ=255) tGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGagcagc < 1:134727/83‑1 (MQ=255) tGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGagcagc > 1:352745/1‑83 (MQ=255) tGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGagcagc > 2:134727/1‑83 (MQ=255) tGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGagcagc < 2:352745/83‑1 (MQ=255) tGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAg > 1:547595/1‑139 (MQ=255) gTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGt < 2:160182/53‑1 (MQ=255) gTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGt > 1:160182/1‑53 (MQ=255) gcagcaAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAgg > 2:392158/1‑139 (MQ=255) cagcaAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGc > 1:325500/1‑139 (MQ=255) cagcaAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGc < 2:569985/139‑1 (MQ=255) agcaAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCa > 1:815549/1‑139 (MQ=255) gcaAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCaa < 2:325500/139‑1 (MQ=255) gACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCCCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAg > 2:221059/1‑139 (MQ=255) aCCCTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAgg < 2:815549/139‑1 (MQ=255) cTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCg < 2:61870/139‑1 (MQ=255) cTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCg < 1:201205/139‑1 (MQ=255) cTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCg < 1:708940/139‑1 (MQ=255) cTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCg < 1:1010196/139‑1 (MQ=255) cTGGGCTTACTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCg < 2:1083844/139‑1 (MQ=255) aCTGCGCGACGGTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTg > 1:933134/1‑139 (MQ=255) ggTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAggg < 1:764334/119‑1 (MQ=255) ggTAAGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAggg > 2:764334/1‑119 (MQ=255) aaGCAGGTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGAACAACGTGaaaa > 2:355520/1‑139 (MQ=255) gTTGACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTg < 2:307103/82‑1 (MQ=255) gTTGACGTGAACCTGGAACTGCAGCAGAGCAGACAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATg > 2:659827/1‑87 (MQ=255) | TGTGATCACCTCACTGAACGGTAAGCCGATCAGCAGCTTTGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGGTTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCTGAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGAACAACGTGAAAA > NC_000913/181885‑182135 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |