Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,049,336	C→T	P1467S (CCG→TCG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,049,336	0	C	T	100.0%	124.1 / NA	39	P1467S (CCG→TCG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/25);  total (14/25)

GCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACG  >  NC_000913/2049201‑2049461                                                                                                                                        |                                                                                                                              gCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCgg                                                                                                                            <  1:339904/139‑1 (MQ=255)        ttACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGc                                                                                                                     <  1:95109/139‑1 (MQ=255)         tACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                    <  1:418534/139‑1 (MQ=255)         tACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                    <  1:527850/139‑1 (MQ=255)                    aaGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGa                                                                                                         <  1:384933/139‑1 (MQ=255)                        cGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGTGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                                                     <  2:317052/139‑1 (MQ=255)                           ggTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAAc                                                                                                           >  1:356052/1‑130 (MQ=255)                           ggTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAAc                                                                                                           <  2:356052/130‑1 (MQ=255)                            gTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGa                                                                                                 <  2:963996/139‑1 (MQ=255)                              ttATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGaca                                                                                               <  2:332313/139‑1 (MQ=255)                                     ttGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGc                                                                                                                     >  2:620612/1‑110 (MQ=255)                                     ttGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGc                                                                                                                     <  1:620612/110‑1 (MQ=255)                                     ttGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAg                                                                                        >  1:789984/1‑139 (MQ=255)                                      tGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                    <  1:475623/110‑1 (MQ=255)                                      tGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                    >  2:475623/1‑110 (MQ=255)                                          ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                   <  1:177596/139‑1 (MQ=255)                                            cAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                                                 <  2:813629/139‑1 (MQ=255)                                                                   aTGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGa                                                          <  2:789984/139‑1 (MQ=255)                                                                    tGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCATCGAAGGATTCGGTGATTGCCGACAATAACGTTCTCCCGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGAt                                                         <  2:1017032/139‑1 (MQ=255)                                                                                  aCCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCATCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAacac                                                <  1:662379/134‑1 (MQ=255)                                                                                  aCCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGc                                                                                                                     >  2:787714/1‑65 (MQ=255)                                                                                  aCCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGc                                                                                                                     <  1:787714/65‑1 (MQ=255)                                                                                  aCCTATGTGCCGAACGTCGCGAATGCTGAAATAACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAacac                                                >  2:662379/1‑134 (MQ=255)                                                                                   ccTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGc                                                                              <  1:1105382/103‑1 (MQ=255)                                                                                   ccTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGc                                                                              >  2:1105382/1‑103 (MQ=255)                                                                                   ccTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGt                                          <  1:922410/139‑1 (MQ=255)                                                                                            cGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAc                                 >  1:4935/1‑139 (MQ=255)                                                                                            cGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAc                                 >  1:329365/1‑139 (MQ=255)                                                                                               aCGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTActct                              <  2:682354/139‑1 (MQ=255)                                                                                                   cgcgAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCg                          >  2:326147/1‑139 (MQ=255)                                                                                                        aTGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGa                                                                                                         >  2:524745/1‑55 (MQ=255)                                                                                                        aTGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGa                                                                                                         <  1:524745/55‑1 (MQ=255)                                                                                                            tGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGAtgtg                 >  1:925047/1‑139 (MQ=255)                                                                                                                aTCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACt                                                                                             <  2:559044/59‑1 (MQ=255)                                                                                                                aTCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACt                                                                                             >  1:559044/1‑59 (MQ=255)                                                                                                                aTCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAgg             <  2:925047/139‑1 (MQ=255)                                                                                                                           aGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACg  <  1:670023/139‑1 (MQ=255)                                                                                                                               tCGAAGGATTCGGTGATTACCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAc                                 <  2:652737/104‑1 (MQ=255)                                                                                                                               tCGAAGGATTCGGTGATTACCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAc                                 >  1:652737/1‑104 (MQ=255)                                                                                                                                        |                                                                                                                              GCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACG  >  NC_000913/2049201‑2049461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑