Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,073,094 T→C L519P (CTG→CCG)  flu → CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,073,0940TC99.3% 509.4 / NA 142L519P (CTG→CCG) fluCP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (57/84);  total (58/84)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

ATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGA  >  NC_000913/2072958‑2073228
                                                                                                                                        |                                                                                                                                      
aTCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccga                                                                                                                                      <  1:333472/139‑1 (MQ=255)
  ccGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacg                                                                                                                                    >  1:91646/1‑139 (MQ=255)
  ccGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacg                                                                                                                                    >  1:469796/1‑139 (MQ=255)
       aaGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGaa                                                                                                                               <  1:989775/139‑1 (MQ=255)
       aaGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGaa                                                                                                                               >  1:944057/1‑139 (MQ=255)
             aTGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAg                                                                                                                         >  1:1010045/1‑139 (MQ=255)
             aTGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAg                                                                                                                         >  2:72576/1‑139 (MQ=255)
              tGCACTCCTGCGGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGt                                                                                                                        <  1:1045416/139‑1 (MQ=255)
               gCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTa                                                                                                                       >  2:616864/1‑139 (MQ=255)
                 aCTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCATCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTAcc                                                                                                                     <  1:752112/139‑1 (MQ=255)
                     cTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTca                                                                                                                 <  2:837838/139‑1 (MQ=255)
                      tGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcac                                                                                                                <  1:120788/139‑1 (MQ=255)
                       gCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacc                                                                                                               <  1:184960/139‑1 (MQ=255)
                          gggAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCAGACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACg                                                                                                            >  2:637856/1‑139 (MQ=255)
                            gAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGa                                                                                                          <  1:1014755/139‑1 (MQ=255)
                            gAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCAGAACGACAGTACCGTCACCACGGa                                                                                                          <  2:27234/139‑1 (MQ=255)
                             aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGAt                                                                                                         >  2:700617/1‑139 (MQ=255)
                             aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGAt                                                                                                         >  1:250751/1‑139 (MQ=255)
                             aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGAt                                                                                                         >  1:129203/1‑139 (MQ=255)
                               gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                                       >  2:1079192/1‑139 (MQ=255)
                               gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                                       >  2:544396/1‑139 (MQ=255)
                               gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                                       >  2:55703/1‑139 (MQ=255)
                                ctctctCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTc                                                                                                      >  1:899895/1‑139 (MQ=255)
                                 tctctcACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCa                                                                                                     <  1:637856/139‑1 (MQ=255)
                                      cACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                <  2:648752/139‑1 (MQ=255)
                                      cACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                <  2:469796/139‑1 (MQ=255)
                                           gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           <  2:577572/139‑1 (MQ=255)
                                                ggCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTa                                                                                      <  1:816733/139‑1 (MQ=255)
                                                ggCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTa                                                                                      <  1:358942/139‑1 (MQ=255)
                                                ggCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTa                                                                                      <  2:915863/139‑1 (MQ=255)
                                                 gCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTAc                                                                                     >  2:447785/1‑139 (MQ=255)
                                                   aGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACAACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                 >  2:787675/1‑93 (MQ=255)
                                                     cGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                 <  1:1093744/91‑1 (MQ=255)
                                                     cGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                 >  2:1093744/1‑91 (MQ=255)
                                                     cGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGcc                                                                                 >  1:174916/1‑139 (MQ=255)
                                                      gTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGccc                                                                                <  2:218768/139‑1 (MQ=255)
                                                           aaaaTCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAg                                                                           <  2:136167/139‑1 (MQ=255)
                                                            aaaTCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGc                                                                          <  1:830783/139‑1 (MQ=255)
                                                                 aGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacc                                                                                                               >  1:396808/1‑97 (MQ=255)
                                                                 aGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacc                                                                                                               <  2:396808/97‑1 (MQ=255)
                                                                  ggAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCg                                                                    >  2:222665/1‑139 (MQ=255)
                                                                   gAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCgg                                                                   <  1:82581/139‑1 (MQ=255)
                                                                   gAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCgg                                                                   >  2:354427/1‑139 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  2:237815/139‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  2:484991/139‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  1:869860/139‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  1:55703/139‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  2:1010045/139‑1 (MQ=255)
                                                                          cacaCTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTAcc                                                                                                                     >  1:1037316/1‑82 (MQ=255)
                                                                          cacaCTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTAcc                                                                                                                     <  2:1037316/82‑1 (MQ=255)
                                                                              ctcactGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACAACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGc                                                        <  1:623691/139‑1 (MQ=255)
                                                                                 actGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTg                                                      <  1:321174/138‑1 (MQ=255)
                                                                                 actGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTg                                                      >  2:321174/1‑138 (MQ=255)
                                                                                   tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGaa                                                                                                                               <  2:1015360/63‑1 (MQ=255)
                                                                                   tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGaa                                                                                                                               >  1:1015360/1‑63 (MQ=255)
                                                                                   tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACa                                                                                                                          >  1:696332/1‑68 (MQ=255)
                                                                                   tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAAc                                                   >  2:619135/1‑139 (MQ=255)
                                                                                    gTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGTCGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACg                                                  <  1:58890/139‑1 (MQ=255)
                                                                                       aGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGaa                                                                                                                               <  2:492748/59‑1 (MQ=255)
                                                                                       aGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                <  2:1067177/90‑1 (MQ=255)
                                                                                         cAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  1:159857/122‑1 (MQ=255)
                                                                                         cAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              >  2:159857/1‑122 (MQ=255)
                                                                                         cAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGcc                                             >  2:944825/1‑139 (MQ=255)
                                                                                         cAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGcc                                             >  1:1007570/1‑139 (MQ=255)
                                                                                           acacCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacc                                                                                                               >  1:136596/1‑71 (MQ=255)
                                                                                           acacCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacc                                                                                                               <  2:136596/71‑1 (MQ=255)
                                                                                             accacACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTg                                         <  1:919064/139‑1 (MQ=255)
                                                                                               cacaCTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTg                                                                        <  1:1078216/106‑1 (MQ=255)
                                                                                               cacaCTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGAc                                       >  1:474894/1‑139 (MQ=255)
                                                                                               cacaCTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGAc                                       >  2:775517/1‑139 (MQ=255)
                                                                                                   cTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCa                                   <  1:944825/139‑1 (MQ=255)
                                                                                                      aCCCAGAAAGCCGTCAACCTGAATGAAGGCacgccga                                                                                                                                      >  2:915659/1‑37 (MQ=255)
                                                                                                      aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           >  1:793929/1‑80 (MQ=255)
                                                                                                      aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcag                                                                >  1:420611/1‑107 (MQ=255)
                                                                                                          aGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGt                            >  2:190685/1‑139 (MQ=255)
                                                                                                            aaaGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCa                          >  2:490037/1‑139 (MQ=255)
                                                                                                                ccGTCAACCTGAATGAAGGCATGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              >  2:679908/1‑99 (MQ=255)
                                                                                                                ccGTCAACCTGAATGAAGGCATGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  1:679908/99‑1 (MQ=255)
                                                                                                                ccGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTa                                                                                      >  2:825977/1‑75 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                <  1:538521/63‑1 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              >  2:829316/1‑97 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  1:829316/97‑1 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg                    >  2:936341/1‑139 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg                    >  1:209551/1‑139 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCACAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGACATTGACCCCACGAATGTCACTCTCg                    >  1:839640/1‑139 (MQ=255)
                                                                                                                     aaCCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCt                 <  2:943701/139‑1 (MQ=255)
                                                                                                                          gaatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGt            <  2:52718/139‑1 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg           <  1:447785/139‑1 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg           <  2:1007570/139‑1 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg           <  1:616864/139‑1 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg           <  1:52491/139‑1 (MQ=255)
                                                                                                                                aGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACtgtg                                                         <  2:417922/88‑1 (MQ=255)
                                                                                                                                aGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACtgtg                                                         >  1:417922/1‑88 (MQ=255)
                                                                                                                                 ggCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                >  1:611989/1‑48 (MQ=255)
                                                                                                                                 ggCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                <  2:611989/48‑1 (MQ=255)
                                                                                                                                 ggCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgca                                                                 >  2:168829/1‑79 (MQ=255)
                                                                                                                                 ggCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg                    >  2:736587/1‑124 (MQ=255)
                                                                                                                                 ggCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg                    <  1:736587/124‑1 (MQ=255)
                                                                                                                                  gCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCTCTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg           <  1:208246/132‑1 (MQ=255)
                                                                                                                                  gCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTAAAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg           >  2:208246/1‑132 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGA  >  NC_000913/2072958‑2073228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: