Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,130,853	C→T	S9N (AGC→AAC)  *406* (TAG→TAA)	wcaD ← wcaC ←	putative colanic acid polymerase putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,130,853	0	C	T	100.0%	117.2 / NA	37	S9N (AGC→AAC)  *406* (TAG→TAA)	wcaD wcaC	putative colanic acid polymerase putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/22);  total (15/22)

TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGC  >  NC_000913/2130717‑2130985                                                                                                                                         |                                                                                                                                     ttttttAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTa                                                                                                                                    <  1:430891/139‑1 (MQ=255)       aaCGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAtt                                                                                                                              <  1:1006787/139‑1 (MQ=255)            tGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGAt                                                                                                                         <  2:1056809/139‑1 (MQ=255)                        aaaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACAt                                                                                                             >  1:84172/1‑139 (MQ=255)                         aaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                            <  1:427042/139‑1 (MQ=255)                                  tAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAg                                                                                                   >  2:435328/1‑139 (MQ=255)                                     cAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCAt                                                                                                <  1:995799/139‑1 (MQ=255)                                            ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                         >  1:500827/1‑139 (MQ=255)                                             gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAtt                                                                                                                              <  1:622123/101‑1 (MQ=255)                                             gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAtt                                                                                                                              >  2:622123/1‑101 (MQ=255)                                             gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                            >  1:1102558/1‑119 (MQ=255)                                             gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                            <  2:1102558/119‑1 (MQ=255)                                             gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATAc                                                                                                           <  2:886991/120‑1 (MQ=255)                                             gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATAc                                                                                                           >  1:886991/1‑120 (MQ=255)                                             gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                         >  1:573194/1‑138 (MQ=255)                                             gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                         <  2:573194/138‑1 (MQ=255)                                              tAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTcc                                                                                       >  1:944495/1‑139 (MQ=255)                                                        aCGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAg                                                                                                                    <  2:1063305/100‑1 (MQ=255)                                                        aCGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAg                                                                                                                    >  1:1063305/1‑100 (MQ=255)                                                         cGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAggcgg                                                                            <  1:170333/139‑1 (MQ=255)                                                              gTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCAt                                                                                                <  2:834085/114‑1 (MQ=255)                                                              gTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCAt                                                                                                >  1:834085/1‑114 (MQ=255)                                                                      gAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGa                                                                                                                                >  1:283839/1‑74 (MQ=255)                                                                      gAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGa                                                                                                                                <  2:283839/74‑1 (MQ=255)                                                                                   aGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTcagcc                                                  >  2:995840/1‑139 (MQ=255)                                                                                       gggCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCg                                              <  2:498075/139‑1 (MQ=255)                                                                                       gggCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCg                                              <  2:500827/139‑1 (MQ=255)                                                                                       gggCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCg                                              <  1:995840/139‑1 (MQ=255)                                                                                        ggCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGt                                             >  1:806636/1‑139 (MQ=255)                                                                                             ttttGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAACTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTAc                                        <  2:798636/139‑1 (MQ=255)                                                                                                            gcagATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTgcg                         <  1:435328/139‑1 (MQ=255)                                                                                                                        gCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGt              <  2:232813/138‑1 (MQ=255)                                                                                                                        gCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGt              >  1:232813/1‑138 (MQ=255)                                                                                                                         cGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGttt            >  1:1020815/1‑139 (MQ=255)                                                                                                                          ggCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTg           <  2:713935/139‑1 (MQ=255)                                                                                                                                 aCAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACt    <  2:84172/139‑1 (MQ=255)                                                                                                                                   aGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGc  <  2:944495/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGC  >  NC_000913/2130717‑2130985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑