Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,131,667	T→C	D135G (GAC→GGC)	wcaC ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,131,667	0	T	C	100.0%	109.8 / NA	32	D135G (GAC→GGC)	wcaC	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/14);  total (18/14)

CCAGTTGGTGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGTCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGTG  >  NC_000913/2131542‑2131798                                                                                                                              |                                                                                                                                    ccaGTTGGTGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTcca                                                                                                                        <  1:723275/139‑1 (MQ=255)       ggTGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTccagaccag                                                                                                                  >  1:482896/1‑139 (MQ=255)             cgcgATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGAc                                                                                                            >  2:580655/1‑139 (MQ=255)                            ccGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGtt                                                                                             >  2:771613/1‑139 (MQ=255)                                  gATAGTTATTTCAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGTTAAAGGTGCAGCGTCCGGTAACACTCCAGCGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATCGTTTTTCAc                                                                                       >  2:749108/1‑139 (MQ=255)                                         aTTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCg                                                                                >  1:770265/1‑139 (MQ=255)                                               ggTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCa                                                                                                                               >  2:994647/1‑86 (MQ=255)                                               ggTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCa                                                                                                                               <  1:994647/86‑1 (MQ=255)                                               ggTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAg                                                                          <  1:771613/139‑1 (MQ=255)                                               ggTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAg                                                                          >  1:823939/1‑139 (MQ=255)                                                   gggCAGTTCTCACATCCTGGTTTCCAGCCTTCGCAACCGTCGGCAAAGTCGCAGCATCCGGTAACACTGCAGTGGCCGTACCGCGTCCAGCCTAAACTCACCTTCGACTTAACGTTTTTCACTTGTTCGCAAAAGCGca                                                                      >  2:438329/1‑139 (MQ=255)                                                    ggCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcac                                                                     >  1:282887/1‑139 (MQ=255)                                                      cATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                   <  2:818733/139‑1 (MQ=255)                                                      cATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                   <  2:823939/139‑1 (MQ=255)                                                       aTTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGCTTTTTCACTTTTTCGCAAAAGCGcaccac                                                                  <  1:741991/139‑1 (MQ=255)                                                                              ccTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                   >  1:479873/1‑115 (MQ=255)                                                                              ccTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                   <  2:479873/115‑1 (MQ=255)                                                                                ttCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcaccac                                                                  >  1:815572/1‑114 (MQ=255)                                                                                ttCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcaccac                                                                  <  2:815572/114‑1 (MQ=255)                                                                                  cGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCtgtg                                       <  2:282887/139‑1 (MQ=255)                                                                                            ggTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGa                             >  1:975651/1‑139 (MQ=255)                                                                                             gTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGaa                            <  2:875415/139‑1 (MQ=255)                                                                                                    cgcAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAg                     >  2:824772/1‑139 (MQ=255)                                                                                                        gCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGacc                 >  1:49239/1‑139 (MQ=255)                                                                                                             cGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACg                                                                 <  1:863212/86‑1 (MQ=255)                                                                                                             cGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACg                                                                 >  2:863212/1‑86 (MQ=255)                                                                                                                  acacTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCg       <  1:153673/139‑1 (MQ=255)                                                                                                                   cacTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGc      <  2:377862/139‑1 (MQ=255)                                                                                                                      tCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCtgt   >  2:750779/1‑139 (MQ=255)                                                                                                                       ccAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCtgtg  >  1:1022509/1‑139 (MQ=255)                                                                                                                        cAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGca                                                                      <  2:721324/70‑1 (MQ=255)                                                                                                                        cAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGca                                                                      >  1:721324/1‑70 (MQ=255)                                                                                                                              |                                                                                                                                    CCAGTTGGTGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGTCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGTG  >  NC_000913/2131542‑2131798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑