Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,189,594	C→T	A439A (GCG→GCA)	yehB ←	putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,189,594	0	C	T	100.0%	145.4 / NA	46	A439A (GCG→GCA)	yehB	putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/21);  total (25/21)

CGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGT  >  NC_000913/2189463‑2189729                                                                                                                                    |                                                                                                                                        cGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACCAATAACGCCAGGCTGCCAGAc                                                                                                                                  >  2:651709/1‑139 (MQ=255)   gCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACGTCGTTTTCATCACGCCGATCATTATCTTTATTGTTTGCCCAAACATGATCGTTAAATGTCCGGTAATCACGCGACTAATAACGCCCGGCTGCCAGAccc                                                                                                                                >  2:733461/1‑138 (MQ=255)                      taatCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGc                                                                                                             >  2:908959/1‑139 (MQ=255)                                tGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGgctgcc                                                                                                                                      <  2:765963/104‑1 (MQ=255)                                tGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGgctgcc                                                                                                                                      >  1:765963/1‑104 (MQ=255)                                          aTCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGc                                                                                                             >  1:732376/1‑119 (MQ=255)                                          aTCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGc                                                                                                             <  2:732376/119‑1 (MQ=255)                                          aTCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCt                                                                                                            <  1:1044514/120‑1 (MQ=255)                                          aTCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCt                                                                                                            >  2:1044514/1‑120 (MQ=255)                                            cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                       >  1:643434/1‑139 (MQ=255)                                            cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                       >  1:855763/1‑139 (MQ=255)                                                               aTTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCa                                                                    <  2:268295/139‑1 (MQ=255)                                                                    cTTTATTGTTTGCCCAAACGT‑ATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAAcaca                                                              >  1:749883/1‑139 (MQ=255)                                                                        aTTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTca                                                                                                          >  2:1037278/1‑92 (MQ=255)                                                                        aTTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTca                                                                                                          <  1:1037278/92‑1 (MQ=255)                                                                          tGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGc                                                                          <  1:996263/122‑1 (MQ=255)                                                                          tGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGc                                                                          >  2:996263/1‑122 (MQ=255)                                                                           gTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCg                                                        <  2:119902/139‑1 (MQ=255)                                                                             ttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                      <  1:1009364/139‑1 (MQ=255)                                                                             ttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                      <  1:908959/139‑1 (MQ=255)                                                                             ttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                      >  2:714355/1‑139 (MQ=255)                                                                              tGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACTTTTGGCTCACAAATTTGTTGTa                                                                                             >  2:325806/1‑99 (MQ=255)                                                                              tGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTa                                                                                             <  1:325806/99‑1 (MQ=255)                                                                                cccAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTc                                                   <  1:229446/139‑1 (MQ=255)                                                                                          aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTca                                                                                                          <  1:1027164/74‑1 (MQ=255)                                                                                          aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTca                                                                                                          >  2:1027164/1‑74 (MQ=255)                                                                                            cGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATg                                       <  1:184272/139‑1 (MQ=255)                                                                                              ttAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCg                                     >  2:1092965/1‑139 (MQ=255)                                                                                                 aaTGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTg                                                                           <  1:895368/98‑1 (MQ=255)                                                                                                 aaTGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTg                                                                           >  2:895368/1‑98 (MQ=255)                                                                                                     tCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACAt                                                             <  2:44709/108‑1 (MQ=255)                                                                                                     tCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACAt                                                             >  1:44709/1‑108 (MQ=255)                                                                                                      ccGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCg                                                        <  1:1050656/112‑1 (MQ=255)                                                                                                      ccGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCg                                                        >  2:1050656/1‑112 (MQ=255)                                                                                                       cGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGCCTTGTTTACTATGCGACTTAGTgg                            >  1:3257/1‑139 (MQ=255)                                                                                                             tCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGa                      <  1:1092965/139‑1 (MQ=255)                                                                                                               aCGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCg                                                        <  1:393365/103‑1 (MQ=255)                                                                                                               aCGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCg                                                        >  2:393365/1‑103 (MQ=255)                                                                                                                    acgaATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAAt               >  1:182228/1‑139 (MQ=255)                                                                                                                       aaTAACGCCAGGCTGCCCGCCCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTCTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTAAGCGCCTTAGTGGCATCGACGGAAATGGc            >  2:566548/1‑139 (MQ=255)                                                                                                                         tAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCg                                                        <  2:37473/93‑1 (MQ=255)                                                                                                                         tAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCg                                                        >  1:37473/1‑93 (MQ=255)                                                                                                                            cGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCaa       <  2:3257/139‑1 (MQ=255)                                                                                                                            cGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCaa       <  2:182228/139‑1 (MQ=255)                                                                                                                               cAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTc                                                   <  2:881556/92‑1 (MQ=255)                                                                                                                               cAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTc                                                   >  1:881556/1‑92 (MQ=255)                                                                                                                               cAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGc    >  2:159411/1‑139 (MQ=255)                                                                                                                                 ggCTGCCAGACCAAAACGCGTCGACGTTTGGCTAACACATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCgt  >  2:812040/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                        CGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGT  >  NC_000913/2189463‑2189729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑