Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 188,939 C→T E190K (GAA→AAA)  map ← methionine aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913188,9390CT100.0% 132.0 / NA 41E190K (GAA→AAA) mapmethionine aminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/19);  total (22/19)

ATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCG  >  NC_000913/188805‑189068
                                                                                                                                      |                                                                                                                                 
atatTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACgg                                                                                                                               >  1:394084/1‑139 (MQ=255)
  atTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGa                                                                                                                             >  2:513437/1‑139 (MQ=255)
   ttGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAg                                                                                                                            >  1:939677/1‑139 (MQ=255)
     gtgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGta                                                                                                                          >  2:715663/1‑138 (MQ=255)
     gtgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTc                                                                                                                          >  2:191727/1‑139 (MQ=255)
      tgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCa                                                                                                                         >  2:589225/1‑139 (MQ=255)
      tgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCa                                                                                                                         <  1:958168/139‑1 (MQ=255)
       gCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACg                                                                                                                                >  1:138684/1‑131 (MQ=255)
       gCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACg                                                                                                                                <  2:138684/131‑1 (MQ=255)
       gCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCAt                                                                                                                        <  1:333427/139‑1 (MQ=255)
       gCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCAt                                                                                                                        >  2:333427/1‑139 (MQ=255)
        cAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATa                                                                                                                       >  1:195815/1‑139 (MQ=255)
        cAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATa                                                                                                                       >  1:300916/1‑139 (MQ=255)
        cAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATa                                                                                                                       >  1:657076/1‑139 (MQ=255)
               gCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTgcagc                                                                                                                <  1:589225/139‑1 (MQ=255)
                cTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACggg                                                                                                                              >  1:635693/1‑124 (MQ=255)
                cTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTgcagc                                                                                                                >  1:998861/1‑138 (MQ=255)
                cTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTgcagc                                                                                                                <  2:998861/138‑1 (MQ=255)
                cTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTATTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACggg                                                                                                                              <  2:635693/124‑1 (MQ=255)
                    gATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTg                                                                                                           <  2:195815/139‑1 (MQ=255)
                      tCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCg                                                                                                         <  2:300916/139‑1 (MQ=255)
                                      tCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGcc                                                                                         <  1:191727/139‑1 (MQ=255)
                                      tCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGcc                                                                                         <  1:513437/139‑1 (MQ=255)
                                                  tCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATAcc                                                                             <  2:939677/139‑1 (MQ=255)
                                                  tCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATAcc                                                                             <  1:520814/139‑1 (MQ=255)
                                                     tGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTAATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtg                                                                          <  1:715663/139‑1 (MQ=255)
                                                        tGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTcc                                                                       >  2:174275/1‑139 (MQ=255)
                                                        tGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGCTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTcc                                                                       <  2:657076/139‑1 (MQ=255)
                                                                         cccGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaacga                                                      >  2:315090/1‑139 (MQ=255)
                                                                           cgcgTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgacg                                                    <  2:844457/139‑1 (MQ=255)
                                                                            gcgTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGcc                                                                                         >  2:806081/1‑101 (MQ=255)
                                                                            gcgTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGcc                                                                                         <  1:806081/101‑1 (MQ=255)
                                                                                       tGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTc                                                              <  1:467210/117‑1 (MQ=255)
                                                                                       tGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTc                                                              >  2:467210/1‑117 (MQ=255)
                                                                                              aTGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGa                                 >  1:102312/1‑139 (MQ=255)
                                                                                              aTGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGa                                 >  1:277295/1‑139 (MQ=255)
                                                                                                              cAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGtt                                                                                                      <  2:894731/54‑1 (MQ=255)
                                                                                                              cAGGTTTCAGTACGACGTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGtt                                                                                                      >  1:894731/1‑54 (MQ=255)
                                                                                                                         acgacgTTGGCTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGAttt      <  1:814496/139‑1 (MQ=255)
                                                                                                                             cgTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCgttaacagc  >  1:1109833/1‑131 (MQ=255)
                                                                                                                             cgTTGGTTTTACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTcgcg  >  1:545206/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                 
ATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCG  >  NC_000913/188805‑189068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: